The gene encoding the type II signal peptidase (SPase II) of was isolated by screening a genomic DNA library of this bacterium for the ability to increase the levels of globomycin resistance in and to complement the growth deficiency at the non-permissive temperature of strain Y815 carrying a temperature-sensitive mutation in its gene for SPase II. The deduced amino acid sequence of the SPase II showed significant similarity with those of other known SPase II enzymes. Activity of the SPase II was demonstrated by a pulse-labelling experiment in In the gene is flanked by the isoleucyl-tRNA synthetase () gene and the pyrimicline biosynthetic () gene cluster, which is known to map at 139° of the chromosome. In the Gram-positive bacteria studied thus far, appears to be the first gene in an operon. The promoter-distal gene () of this operon specifies a hypothetical protein in bacteria and yeast.


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