1887

Abstract

The genomic and cDNA copy of the TATA-binding protein (TBP) gene from the filamentous fungus have been cloned. The gene is interrupted by four introns, one of which is in the long 5' untranslated region of 615 bp. The transcription initiation site was established and the levels of mRNA were analysed under diverse growth conditions and found to vary severalfold. The gene encodes a protein of 268 amino acids composed of an N-terminal domain of 88 amino acids with no significant homology to other TBPs and a C-terminal domain of 180 amino acids with about 95% homology to other fungal TBPs. A cDNA clone under the yeast promoter was able to substitute for the yeast TBP gene however, the transformants obtained grew poorly at 35°C and on galactose and glycerol at 30°C, though they could grow in the presence of copper ions or aminotriazole at this temperature. This phenotype may be the result of altered function of TBP in certain yeast transcription activation pathways.

Keyword(s): Aspergillus nidulans and TBP
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1997-04-01
2021-05-18
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References

  1. Altschul S. F., Gish, W., Miller W., Myers E. W. 1990; Basic local alignment search tool.. J Mol Biol 215:403–410
    [Google Scholar]
  2. Ballance D. J. 1986; Sequences important for gene expression in filamentous fungi.. Yeast 2:229–236
    [Google Scholar]
  3. Boeke J. D., Trueheart J., Natsoulis G., Fink G. R. 1987; 5- Fluoorotic acid as a selective agent in yeast molecular genetics.. Methods Enzymol 154:164–175
    [Google Scholar]
  4. Chasman D. I., Flaherty K. M., Sharp P. A., Kornberg R. D. 1993; Crystal structure of yeast TATA-binding protein and model for interaction with DNA.. Proc Natl Acad Sci USA 90:8174–8178
    [Google Scholar]
  5. Chen D. C., Yang B. C., Kuo F. T. 1992; One-step transformation of yeast in stationary phase.. Curr Genet 21:83–84
    [Google Scholar]
  6. Connerton i. f., Fincham j. R., Sandeman R. A., Hynes M. J. 1990; Comparison and cross-species expression of the acetyl coA synthetase genes of the ascomycete fungi, Aspergillus nidulans and Neurospora crassa.. Mol Microbiol 4:451–460
    [Google Scholar]
  7. Cormack B. P., Strubin M., Stargell L. A., Struhl K. 1994; Conserved and nonconserved functions of the yeast and human TATA binding proteins.. Genes Dev 8:1335–1343
    [Google Scholar]
  8. Damiani R. D., Wessler S. R. 1993; An upstream open reading frame represses expression of Lc, a member of the R/B family of maize transcriptional activators.. Proc Natl Acad Sci USA 90:8244–8248
    [Google Scholar]
  9. Dhawale S. S., Lane A. C. 1993; Compilation of sequence specific DNA binding proteins implicated in transcriptional control in fungi... Nucleic Acids Res 21:5537–5546
    [Google Scholar]
  10. Doonan J. H., Morris N. R. 1989; The bimG gene of Aspergillus nidulans, required for completion of anaphase, encodes a homolog of mammalian phosphoprotein phosphatase 1.. Cell 57:987–996
    [Google Scholar]
  11. Dowzer C. E., Kelly J. M. 1991; Analysis of the creA gene, a regulator of carbon catabolite repression in Aspergillus nidulans.. Mol Cell Biol 11:5701–5709
    [Google Scholar]
  12. Felsenstein J. 1989; PHYLIP- phylogeny inference package version 3.. Cladistics 5:164–166
    [Google Scholar]
  13. Fikes J.D., Becker D., Winston F., Guarente L. 1990; Striking conservation of TFIID in Schizosaccharomyces pombe and Saccharomyces cerevisiae.. Nature 346:291–294
    [Google Scholar]
  14. de Graaff L.H., van de Broeck H. C., Ooijen A. J. J., Visser J. 1994; Regulation of the xylanase encoding xlnA gene of Aspergillus tubigensis.. Mol Microbiol 12:479–490
    [Google Scholar]
  15. Gurr S.J., Unkles S.E, Kinghorn J.R. 1987; The structure and organization of nuclear genes of filamentous fungi. In Gene Structure in Eukaryotic Microbes. Edited by J. R. Kinghorn. Oxford . IRL Press
    [Google Scholar]
  16. Hahn S., Buratowski S., Sharp P.A., Guarente L. 1989; Isolation of the gene encoding the yeast TATA binding protein TFIID: a gene identical to the SPT15 suppressor of Ty element insertions.. Cell 58:1173–1181
    [Google Scholar]
  17. Hancock J.M. 1993; Evolution of sequence repetition and gene sequences in TATA-binding protein TBP (TFIID).. Nucleic Acids Res 21:2823–2830
    [Google Scholar]
  18. Hernandez N. 1993; TBP, a universal eukaryotic transcription factor?. Genes Dev 7:1291–1308
    [Google Scholar]
  19. Higgins D.G., Sharp P.M. 1988; CLUSTAL: a package for performing multiple sequence alignment on a microcomputer.. Gene 73:237–244
    [Google Scholar]
  20. Hill J.G., Morris D.R. 1993; Cell-specific translational regulation of S-adenosylmethionine decarboxylase mRNA. Dependence on translation and the coding capacity of the cis-acting upstream open reading frame.. J Biol Chem 268:726–731
    [Google Scholar]
  21. Humphrey T., Birse C.E., Proudfoot N.J. 1994; RNA 3´ end signals of the S. pombe ura4 gene comprise a new site determining an efficiency element.. EMBO J 13:2441–2451
    [Google Scholar]
  22. Kawamoto T., Makino K., Niwa H., Sugiyama H., Kimura S., Amemura M., Nakata H., Niwa A., Kakunaga T. 1988; Identification of the human β-actin enhancer and its binding factor.. Mol Cell Biol 8:267–272
    [Google Scholar]
  23. Kozak M. 1987; Effects of intercistronic length on the efficiency of reinitiation by eukaryotic ribosomes.. Mol Cell Biol 7:3438–3445
    [Google Scholar]
  24. Kozak M. 1991; Structural features in eukaryotic mRNAs that modulate the initiation of translation.. Mol Cell Biol 266:19867–19870
    [Google Scholar]
  25. Lloyd A. T., Sharp P.M. 1991; Codon usage in Aspergillus nidulans.. Mol Gen Genet 230:288–294
    [Google Scholar]
  26. Marchuk D., Drumm M., Saulino A., Collins F. S. 1991; Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products.. Nucleic Acids Res 19:1154
    [Google Scholar]
  27. Miller K.Y., Wu J., Miller B.L. 1991; stuA is required for cell pattern formation in Aspergillus.. Genes Dev 6:1770–1782
    [Google Scholar]
  28. Nakashima K., Nobuhisa I., Deshimaru M., Ogawa T., Shimoshigashi Y., Fukumaki Y., Hattori M., Sakaki Y., Hattori S., Ohno M. 1995; Structures of genes encoding TATA-box binding proteins from Trimesurus gramineus and T . flavoviridis snakes.. Gene 152:209–213
    [Google Scholar]
  29. Oliviera C.C., McCarthy J.E. 1995; The relationship between eukaryotic translation and mRNA stability. A short upstream open reading frame strongly inhibits translational initiation and greatly accelerates mRNA degradation in the yeast Saccharomyces cerevisiae.. J Biol Chem 270:8936–8943
    [Google Scholar]
  30. Pearson L., Meagher R.B. 1990; Diverse soybean actin transcripts contain a large intron in the 5´ untranslated leader : structural similarity to vertebrate muscle actin genes.. Plant Mol Biol 14:513–526
    [Google Scholar]
  31. Pontecorvo G., Roper J.A., Hemmons L.M., MacDonald R.D., Bufton A.W.J. 1990; Diverse soybean actin transcripts contain a large intron in the 5´ untranslated leader : structural similarity to vertebrate muscle actin genes.. Plant Mol Biol 14:513–526
    [Google Scholar]
  32. Sambrook J., Fritsch E.F., Maniatis T. 1989; Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY:. Cold Spring Harbor Laboratory
    [Google Scholar]
  33. Sharp P. M., Tuohy T.M.F., Mosurski K.R. 1986; Codon usage in yeast : cluster analysis clearly differentiates highly and lowly expressed genes.. Nucleic Acids Res 14:5125–5143
    [Google Scholar]
  34. Stargell L. A., Struhl K. 1995; The TBP-TFIIA interaction in the response to acidic activators in vivo. Science 269:75–78
    [Google Scholar]
  35. Sunkin S. M., Stringer J. R. 1995; Transcription factor genes from rat Pneumocystis carinii.. J Eukaryot Microbiol 42:12–19
    [Google Scholar]
  36. Vega Laso M. R., Zhu D., Sagliocco F., Brown A.J.P., Tuite M.F., McCarthy J.E.G. 1993; Inhibition of translational initiation in the yeast Saccharomyces cerevisiae as a function of the stability of hairpin structures in the mRNA leader.. J Biol Chem 268:6453–6462
    [Google Scholar]
  37. Vogel J. M., Roth B., Cigan M., Freeling M. 1993; Expression of two maize TATA binding protein genes and function of the encoded TBP proteins by complementation in yeast.. Plant Cell 5 5:1627–1638
    [Google Scholar]
  38. Yelton M. M., Timberlake W.E., Van Den Hondel C.A.M.J.J. 1985; A cosmid for selecting genes by complementation in Aspergillus nidulans : selection of the developmentally regulated yA locus.. Proc Nut1 Acad Sci USA 82:834–838
    [Google Scholar]
  39. Zaret K. S., Sherman F. 1982; DNA sequence required for efficient transcription termination in yeast.. Cell 28:563–573
    [Google Scholar]
  40. Zuker M. 1989; On finding all suboptimal foldings of an RNA molecule.. Science 244:48–52
    [Google Scholar]
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