1887

Abstract

The gene encoding the catalytic subunit of cAMP-dependent protein kinase has been isolated from the industrially important filamentous fungus A probe for screening phage libraries was generated by a polymerase chain reaction using degenerate primers. cDNA and genomic DNA clones were isolated and sequenced. An open reading frame of 1440 bp, interrupted by three short introns, encodes a polypeptide of 480 amino acids with a calculated molecular mass of 53813 Da. The cAMP-dependent protein kinase catalytic subunit (PKA-C) from has a 126 amino acid extension at the N-terminus compared to the PKA-C of higher eukaryotes that - except for the first 15 amino acids, which are homologous to the PKA-C - shows no significant similarity to the N-terminal extension of PKA-C of other lower eukaryotes. The catalytic core of PKA-C of A. shows extensive homology with the PKA-C isolated from all other eukaryotes. Low-stringency hybridization did not reveal any other homologue in The cloned was used for transformation of leading to increased levels of mRNA and PKA-C activity. Transformants overexpressing were phenotypically different with respect to growth, showing a more compact colony morphology, accompanied by a more dense sporulation, especially on media containing trehalose and glycerol. A number of transformants also showed a strongly reduced or complete absence of sporulation. This phenotype was quickly lost upon propagation of the strains.

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1997-04-01
2024-03-28
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