1887

Abstract

Twenty-three of the most prominent spots which are visible on two-dimensional (2-D) protein gels of crude extracts were selected as marker spots for the construction of a 2-D protein index. N-terminal sequencing of the corresponding proteins resulted in the identification of enzymes involved in glycolysis, TCA cycle, pentose phosphate cycle, amino acid metabolism, nucleotide biosynthesis and translation. Using computer analysis of the 2-D protein gels, most of these metabolic enzymes were found to be synthesized at a reduced rate after different stresses and glucose starvation. Such an approach permits a rapid and global evaluation of the regulation of different branches of metabolism in response to various physiological conditions.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-143-3-991
1997-03-01
2024-10-04
Loading full text...

Full text loading...

/deliver/fulltext/micro/143/3/mic-143-3-991.html?itemId=/content/journal/micro/10.1099/00221287-143-3-991&mimeType=html&fmt=ahah

References

  1. Antelmann H., Bernhardt J., Schmid R. 1995; A gene at 333 degrees on the Bacillus subtilis chromosome encodes the newly identified σB-dependent general stress protein GspA.. J Bacteriol 177:3540–3545
    [Google Scholar]
  2. Bernhardt J., Völker U., Völker A., Antelmann H., Schmid R., Mach H., Hecker M. 1997; Specific and general stress proteins in Bacillus subtilis - a two-dimensional protein electrophoresis study.. Microbiology 143:000–000
    [Google Scholar]
  3. Cashel M., Rudd K. 1987; The stringent response.. In Escherichia coli and Salmonella typhimurium-Cellular and Molecular Biology pp. 1410–1438 Edited by Neidhardt F. C., Ingraham J. L., Brooks-Low K., Magasanik B., Schaechter M., Umbarger H. E. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  4. Corbett J.M., Wheeler C.H., Baker C.S., Yacoub M.H., Dunn M.J. 1994; The human myocardial two-dimensional gel protein database: Update 1994.. Electrophoresis 15:1459–1465
    [Google Scholar]
  5. Devine K.M. 1995; The Bacillus subtilis genome project: aims and progress.. Trends Biotechnol 13:210–216
    [Google Scholar]
  6. Fleischmann R. D. others 1995; Whole-genome random sequencing and assembly of Haemophilus influenzae Rd.. Science 269:496–512
    [Google Scholar]
  7. Fraser C. M. others 1995; The minimal gene complement of Mycoplasma genitalium. . Science 270:397–403
    [Google Scholar]
  8. Garrels J.I. 1995; YPD-a database for the proteins of Saccharomyces cerevisiae. . Nucleic Acids Res 24:46–49
    [Google Scholar]
  9. Golaz O., Hughes G.J., Frutiger S., Paquet N., Bairoch A., Pasquali C., Sanchez J.C., Tissot J.D., Appel R.D., Walzer C. 1993; Plasma and red blood cell protein maps: update 1993.. Electrophoresis 14:1223–1231
    [Google Scholar]
  10. Graumann P., Schröder K., Schmid R., Marahiel M.A. 1996; Cold shock stress-induced proteins in Bacillus subtilis. . J Bacteriol 178:4611–4619
    [Google Scholar]
  11. Hanson R.S., Cox D.P. 1967; Effects of different nutritional conditions on the synthesis of tricarboxylic acid cycle enzymes.. J Bacteriol 93:1777–1787
    [Google Scholar]
  12. Hecker M., Schumann W., Völker U. 1996; Heat-shock and general stress response in Bacillus subtilis. . Mol Microbiol 19:417–428
    [Google Scholar]
  13. Hughes G.J., Frutiger S., Paquet N., Pasquali C., Sanchez J.C., Tissot J.D., Bairoch A., Appel R.D., Hochstrasser D.F. 1993; Human liver protein map: update 1993.. Electrophoresis 14:1216–1222
    [Google Scholar]
  14. Kunst F., Devine K. 1991; The project of sequencing the entire Bacillus subtilis genome.. Res Microbiol 142:905–912
    [Google Scholar]
  15. Lemkin P.F., Orr G.A., Goldstein M.P., Creed G.J., Myrick J.E., Merril C.R. 1995; The Protein Disease Database of human body fluids: II. Computer methods and data issues.. Appl Theor Electrophor 5:55–72
    [Google Scholar]
  16. Merril C.R., Goldstein M.P., Myrick J.E., Creed G.J., Lemkin P.F. 1995; The Protein Disease Database of human body fluids: I. Rationale for the development of this database.. Appl Theor Electrophor 5:49–54
    [Google Scholar]
  17. Miller B.S., Kennedy T.E., Streips U.N. 1991; Molecular characterisation of specific heat shock proteins in Bacillus subtilis. . Curr Microbiol 22:231–236
    [Google Scholar]
  18. Rider M.H., Puype M., VanDamme J., Gevaert K., DeBoeck S., D’Alayer J., Rasmussen H.H., Celis J.H., Vanderkerckhove J. 1995; An agarose-based gel-concentration system for microsequence and mass spectrometric characterisation of proteins previously purified in polyacrylamide gels starting at low picomole levels.. Eur J Biochem 230:258–266
    [Google Scholar]
  19. Schaegger H., Von Jagow G. 1987; Tricine-sodium- dodecylsulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1-100 kDa.. Anal Biochem 166:368–379
    [Google Scholar]
  20. Smith L., Paress P., Cabane K., Dubnau E. 1980; Genetics and physiology of the rel system of Bacillus subtilis. . Mol Gen Genet 179:271–279
    [Google Scholar]
  21. Sonenshein A. 1989; Metabolic regulation of sporulation and other stationary phase phenomena.. In Regulation of Prokaryotic Development pp. 109–130 Edited by Issar Smith, Ralph A., Slepecky, Peter Setlow. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  22. Stülke J., Hanschke R., Hecker M. 1993; Temporal activation of β-glucanase synthesis in Bacillus subtilis is mediated by the GTP pool.. J Gen Microbiol 139:2041–2045
    [Google Scholar]
  23. VanBogelen R.A., Sankar P., Clark R.L., Bogan J.A., Neidhardt F.C. 1992; The Gene-Protein Database of Escherichia coli- Edition 5.. Electrophoresis 13:1014–1054
    [Google Scholar]
  24. V϶lker U., Engelmann S., Maul B., Riethdorf S., Völker A., Schmid R., Mach H., Hecker M. 1994; Analysis of the induction of general stress proteins of Bacillus subtilis. . Microbiology 140:741–752
    [Google Scholar]
/content/journal/micro/10.1099/00221287-143-3-991
Loading
/content/journal/micro/10.1099/00221287-143-3-991
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error