RT Journal Article SR Electronic(1) A1 Müler, Jörg P. A1 An, Zhidong A1 Merad, Tarek A1 Hancock, Ian C. A1 Harwood, Colin R.YR 1997 T1 Influence of Bacillus subtilis phoR on cell wall anionic polymers JF Microbiology, VO 143 IS 3 SP 947 OP 956 DO https://doi.org/10.1099/00221287-143-3-947 PB Microbiology Society, SN 1465-2080, AB In Bacillus subtilis the Pho regulon is controlled by a sensor and regulator protein pair, PhoR and PhoP, that respond to phosphate concentrations. To facilitate studies of the Pho regulon, a strain with an altered PhoR protein was isolated by in vitro mutagenesis. The mutation in this strain (phoR12) leads to the production of a PhoR sensor kinase that, unlike the wild-type, is functionally active in phosphate-replete conditions. The lesion in phoR12 was shown to be a single base change that results in an Arg to Ser substitution in a region of PhoR that is highly conserved in histidine sensor kinases. While a phoR-negative mutant was unable to induce the synthesis of cell wall teichuronic acid under phosphate-limited conditions, the phoR12 mutant showed a relative increase in teichuronic acid and a decrease in teichoic acid, even under phosphate-replete conditions. The latter suggests that some or all of the genes required for teichuronic acid synthesis are members of the Pho regulon., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-143-3-947