1887

Abstract

The gene coding for the homologue of LexA has been cloned and sequenced. Amino acids required for autocatalytic cleavage are conserved, whereas those important for specific DNA binding are not, when compared with LexA. The transcriptional start site was mapped and a DNA sequence motif was identified which resembled the consensus Cheo box sequence involved in the regulation of DNA-damage-inducible genes in . The LexA protein was overexpressed in and purified by means of a His tag. The purifed LexA was shown to bind to the Cheo box sequence found upstream of its own gene.

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1997-03-01
2021-05-10
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