%0 Journal Article %A Qian, Ny %A Stanley, Grant A. %A Bunte, Annicka %A Rdstrm, Peter %T Product formation and phosphoglucomutase activities in Lactococcus lactis: cloning and characterization of a novel phosphoglucomutase gene %D 1997 %J Microbiology, %V 143 %N 3 %P 855-865 %@ 1465-2080 %R https://doi.org/10.1099/00221287-143-3-855 %K Lactococcus lactis %K maltose %K metabolism %K -phosphoglucomutase %I Microbiology Society, %X Maltose metabolism in Lactococcus lactis involves the conversion of -glucose 1-phosphate to glucose 6-phosphate, a reaction which is reversibly catalysed by a maltose-inducible and glucose-repressible -phosphoglucomutase (-PGM). The gene encoding -PGM (pgmB) was cloned from a genomic library of L. lactis using antibodies. The nucleotide sequence of a 5695 bp fragment was determined and six ORFs, including the pgmB gene, were found. The gene expressed a polypeptide with a calculated molecular mass of 24210 Da, which is in agreement with the molecular mass of the purified -PGM (25 kDa). A short sequence at the N-terminus was found to be similar to known metal-binding domains. The expression of -PGM in L. lactis was found to be induced also by trehalose and sucrose, and repressed by lactose in the growth medium. This indicates that -PGM does not serve solely to degrade maltose, but that it is also involved in the metabolism of other carbohydrates. The specific activity of a-PGM during fermentation was dependent on the maltose concentration in the medium. The maximum specific activity of -PGM increased by a factor of 4.6, and the specific growth rate by a factor of 7, when the maltose concentration was raised from 0.8 to 11.0 g I-1. Furthermore, a higher amount of lactate produced relative to formate, acetate and ethanol was observed when the initial maltose concentration in the medium was increased. The specific activity of -PGM responded similarly to -PGM, but the magnitude of the response was lower. Preferential sugar utilization and a- and -PGM suppression was observed when L. lactis was grown on the substrate combinations glucose and maltose, or lactose and maltose; maltose was the least-preferred sugar. In contrast, galactose and maltose were utilized concurrently and both PGM activities were high throughout the fermentation. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-143-3-855