1887

Abstract

Maltose metabolism in involves the conversion of -glucose 1-phosphate to glucose 6-phosphate, a reaction which is reversibly catalysed by a maltose-inducible and glucose-repressible -phosphoglucomutase (-PGM). The gene encoding -PGM () was cloned from a genomic library of using antibodies. The nucleotide sequence of a 5695 bp fragment was determined and six ORFs, including the gene, were found. The gene expressed a polypeptide with a calculated molecular mass of 24210 Da, which is in agreement with the molecular mass of the purified -PGM (25 kDa). A short sequence at the N-terminus was found to be similar to known metal-binding domains. The expression of -PGM in was found to be induced also by trehalose and sucrose, and repressed by lactose in the growth medium. This indicates that -PGM does not serve solely to degrade maltose, but that it is also involved in the metabolism of other carbohydrates. The specific activity of a-PGM during fermentation was dependent on the maltose concentration in the medium. The maximum specific activity of -PGM increased by a factor of 4.6, and the specific growth rate by a factor of 7, when the maltose concentration was raised from 0.8 to 11.0 g I. Furthermore, a higher amount of lactate produced relative to formate, acetate and ethanol was observed when the initial maltose concentration in the medium was increased. The specific activity of -PGM responded similarly to -PGM, but the magnitude of the response was lower. Preferential sugar utilization and a- and -PGM suppression was observed when was grown on the substrate combinations glucose and maltose, or lactose and maltose; maltose was the least-preferred sugar. In contrast, galactose and maltose were utilized concurrently and both PGM activities were high throughout the fermentation.

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1997-03-01
2021-10-27
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