1887

Abstract

Maltose metabolism in involves the conversion of β-glucose 1-phosphate to glucose 6-phosphate, a reaction which is reversibly catalysed by a maltose-inducible and glucose-repressible β-phosphoglucomutase (β-PGM). The gene encoding β-PGM () was cloned from a genomic library of using antibodies. The nucleotide sequence of a 5695 bp fragment was determined and six ORFs, including the gene, were found. The gene expressed a polypeptide with a calculated molecular mass of 24210 Da, which is in agreement with the molecular mass of the purified β-PGM (25 kDa). A short sequence at the N-terminus was found to be similar to known metal-binding domains. The expression of β-PGM in was found to be induced also by trehalose and sucrose, and repressed by lactose in the growth medium. This indicates that β-PGM does not serve solely to degrade maltose, but that it is also involved in the metabolism of other carbohydrates. The specific activity of α-PGM during fermentation was dependent on the maltose concentration in the medium. The maximum specific activity of β-PGM increased by a factor of 4.6, and the specific growth rate by a factor of 7, when the maltose concentration was raised from 0.8 to 11.0 g I. Furthermore, a higher amount of lactate produced relative to formate, acetate and ethanol was observed when the initial maltose concentration in the medium was increased. The specific activity of β-PGM responded similarly to β-PGM, but the magnitude of the response was lower. Preferential sugar utilization and α- and β-PGM suppression was observed when was grown on the substrate combinations glucose and maltose, or lactose and maltose; maltose was the least-preferred sugar. In contrast, galactose and maltose were utilized concurrently and both PGM activities were high throughout the fermentation.

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/content/journal/micro/10.1099/00221287-143-3-855
1997-03-01
2019-10-15
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-143-3-855
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