1887

Abstract

Fire blight is a necrotic disease caused by the bacterium which affects pears, apples and ornamentals including and The disease can be only partially controlled, through the use of resistant genotypes, cultural measures and antibacterial compounds, thus other methods must be investigated. It has long been established that avirulent isolates of the pathogen can control the disease, under experimental conditions. However, field use of avirulent isolates is not acceptable because of their unknown genetic stability. The protective ability under controlled conditions of genetically characterized avirulent insertion mutants of was examined. A bioassay on apple seedlings was used for the determination of the protective ability of 34 insertion mutants (). Some protective effect could be observed with most of the mutants tested and was dependent on the avirulent/virulent inoculum ratio as well as on the level of virulence of the pathogen; a minimal concentration of the avirulent mutant was necessary to give a significant level of protection. An early competition between avirulent and virulent strains for putative infection sites might be involved. For six of the mutants tested, the protective ability was particularly high and might be related to the alteration of regulatory functions of hrp genes. Results obtained with Ams and Ams Hrp mutants suggested that the bacterial exopolysaccharide might play a role in the protection.

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1997-02-01
2021-10-20
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References

  1. Aldwinckle H.S., Beer S.V. 1979; Fire blight and its control.. Hortic Rev 1:423–475
    [Google Scholar]
  2. Averre C.W., Kelman A. 1964; Severity of bacterial wilt as influenced by ratio of virulent to avirulent cells of Pseudomonas solanacearum in inoculum.. Phytopathology 54:779–783
    [Google Scholar]
  3. Barny M.-A., Guinebretiére M.-H., Marçais B., Coissac E., Paulin J.-P., Laurent J. 1990; Cloning of a large gene cluster involved in Erwinia amylovora CFBP1430 virulence.. Mol Microbiol 4:777–786
    [Google Scholar]
  4. Bauer D.W., Beer S.V. 1991; Further characterization of an hrp gene cluster of Erwinia amylovora. . Mol Plant-Microbe Interact 4:493–499
    [Google Scholar]
  5. Beer S.V., Rundle J.R., Norelli J. 1987; Orchard evaluation of five strains of Erwinia herbicola for control of blossom infection.. Acta Hortic217–219 (abstract).
    [Google Scholar]
  6. Belleman P., Geider K. 1992; Localization of transposon insertions in pathogenicity mutants of Erwinia amylovora and their biochemical characterization.. J Gen Microbiol 138:931–940
    [Google Scholar]
  7. Bernhard F., Coplin D.L., Geider K. 1993; A gene cluster for amylovoran synthesis in Erwinia amylovora: characterization and relationship to cps genes in Erwinia stewartii. . Mol Gen Genet 239:158–168
    [Google Scholar]
  8. Bogdanove A.J., Wei Z.-M., Zhao L., Beer S.V. 1996; Erwinia amylovora secretes harpin via a type III pathway and contains a homolog of yopN of Yersinia spp.. J Bacteriol 178:1720–1730
    [Google Scholar]
  9. Bostock R.M., Stermer B.A. 1989; Perspectives on wound healing in resistance to pathogens.. Annu Rev Phytopathol 27:343–371
    [Google Scholar]
  10. Bradley D.J., Kjellbom P., Lamb C.J. 1992; Elicitor- and wound-induced oxidative cross-linking of a proline-rich plant cell wall protein: a novel, rapid defense response.. Cell 70:21–30
    [Google Scholar]
  11. Bugert P., Geider K. 1995; Molecular analysis of the ams operon required for exopolysaccharide synthesis of Erwinia amylovora. . Mol Microbiol 15:917–933
    [Google Scholar]
  12. Clarke H.R.G., Leigh J.A., Douglas C.J. 1992; Molecular signals in the interactions between plants and microbes.. Cell 71:91–99
    [Google Scholar]
  13. Cooper R.M., Youle D., Katerinas A., Fox C. 1990; Host induced virulence in Erwinia amylovora. . Acta Hortic 273:267–274
    [Google Scholar]
  14. Frey P., Prior P., Marie C., Kotoujansky A., Trigalet-Démery D., Trigalet A. 1994; Hrp mutants of Pseudomonas sola- nacearum as potential biocontrol agents of tomato bacterial wilt.. Appl Environ Microbiol 60:3175–3181
    [Google Scholar]
  15. Goodman R.N. 1967; Protection of apple stem tissue against Erwinia amylovora infection by avirulent strains and three other bacterial species.. Phytopathology 57:22–24
    [Google Scholar]
  16. Gough C.L., Genin S., Zischek C., Boucher C.A. 1992; hrp genes of Pseudomonas solanacearum are homologous to pathogenicity determinants of animal pathogenic bacteria and are conserved among plant pathogenic bacteria.. Mol Plant-Microbe Interact 5:348–389
    [Google Scholar]
  17. Hignett R.C. 1987; Inhibition of fireblight disease by injection of D-galactose or L-fucose into susceptible apple plants.. Physiol Mol Plant Pathol 30:131–138
    [Google Scholar]
  18. Hignett R.C. 1988; Effects of growth conditions on the surface structures and extracellular products of virulent and avirulent forms of Erwina amylovora. . Physiol Mol Plant Pathol 32:387–394
    [Google Scholar]
  19. Isenbeck M., Schulz F.A. 1985; Biological control of fire blight (Erwinia amylovora (Burr.) Winslow et al.) on ornamentals. 1. Control of the pathogen by antagonistic bacteria.. Phytopathol Z 113:324–333
    [Google Scholar]
  20. Kearns L.P., Hale C.N. 1993; Biological control of fire blight by Erwinia herbicola: survival of applied bacteria in orchard and glasshouse trials.. Acta Hortic338–333
    [Google Scholar]
  21. King E.O., Ward M.K., Raney D.E. 1954; Two simple media for the demonstration of pyocyanin and fluorescein.. J Lab Clin Med 44:301–307
    [Google Scholar]
  22. Lippincott B.B., Lippincott J.A. 1969; Bacterial attachment to a specific wound site as an essential stage in tumor initiation by Agrobacterium tumefaciens. . J Bacteriol 97:620–628
    [Google Scholar]
  23. Loper J.E., Henkels M.D., Roberts R.G., Grove G.G., Willet M.J., Smith T.J. 1991; Evaluation of streptomycin, oxy- tetracycline, and copper resistance of Erwinia amylovora isolated from pear orchards in Washington State.. Plant Dis 75:287–290
    [Google Scholar]
  24. Matta A. 1991; The role of cell injury in induced resistance.. Petria 1:85–98
    [Google Scholar]
  25. McIntyre J.L., Kuc J., Williams E.B. 1973; Protection of pear against fire blight by bacteria and bacterial sonicates.. Phytopathology 63:872–877
    [Google Scholar]
  26. Menggad M. 1994 Etude génétique et moleculaire de la région chromosomique ams impliquée dans le pouvoir pathogène et la production de l‘exopolysaccharide acide de la capsule d‘Erwinia amylovora. Thèse de Docteur en Sciences Universite de Paris XI; Orsay, France.:
    [Google Scholar]
  27. Moller W. J., Schroth M. N., Thomson S. V. 1981; Thescenario of fire blight and streptomycin resistance.. Plant Dis 65:563–568
    [Google Scholar]
  28. Norelli J.L., Aldwinckle H.S., Beer S.V. 1984; Differential host-pathogen interactions among cultivars of apple and strains of Erwinia amylovora. . Phytopathology 74:136–139
    [Google Scholar]
  29. Paulin J.-P. 1978; Biological control of fireblight: preliminary experiments.. In Proceedings of the 4th International Conference on Plant Pathogenic Bacteria Angers, France p. 525. Angers:: Station de Pathologie V′g′tale et Phytobact′riologie.;
    [Google Scholar]
  30. Paulin J.-P., Samson R. 1973; Le feu bact′rien en France.II. Caract′res des souches d’Erwinia amylovora (Burrill) Winslow et al., 1920, isol′es du foyer franco-beige.. Ann Phytopathol 5:389–397
    [Google Scholar]
  31. Paulin J.-P., Lachaud G., Lespinasse Y. 1993; Role of the agressiveness of strains of Erwinia amylovora in the experimental evaluation of susceptibility of apple cultivars to fire blight.. Acta Hortic 338:375–376 (abstract)
    [Google Scholar]
  32. Steinberger E.M., Beer S.V. 1988; Creation and complementation of pathogenicity mutants of Erwinia amylovora. . Mol Plant-Microbe Interact 1:135–144
    [Google Scholar]
  33. Tharaud M., Baudouin E., Paulin J.-P. 1993; Protection against fire blight by avirulent strains of Erwinia amylovora: modulation of the interaction by avirulent mutants.. Acta Hortic 338:321–327
    [Google Scholar]
  34. Tharaud M., Baudouin E., Paulin J.-P. 1994a; Protection against fire blight by an avirulent strain of Erwinia amylovora: possible role of the host in the interaction.. In Plant Pathogenic Bacteria pp. 943–949 Lemattre M., Freigoun S., WRudolph S., Swings J. G. Edited by Paris:: INRA (Les Colloques, no. 66).;
    [Google Scholar]
  35. Tharaud M., Menggad M., Paulin J.-P., Laurent J. 1994b; Virulence, growth, and surface characteristics of Erwinia amylovora mutants with altered pathogenicity.. Microbiology 140:659–669
    [Google Scholar]
  36. Thomson S.V., Schroth M.N., Moller W.J., Reil W.O. 1976; Efficacy of bactericides and saprophytic bacteria in reducing colonization and infection of pear flowers by Erwinia amylovora. . Phytopathology 66:1457–1459
    [Google Scholar]
  37. Trigalet A., Trigalet-D6mery D. 1990; Use of avirulent mutants of Pseudomonas solanacearum for the biological control of bacterial wilt of tomato plants.. Physiol Mol Plant Pathol 36:27–38
    [Google Scholar]
  38. Wei Z.-M., Beer S.V. 1993; HrpL of Erwinia amylovora functions in secretion of harpin and is a member of a new protein family.. J Bacteriol 175:7958–7967
    [Google Scholar]
  39. Wei Z.-M., Beer S.V. 1995; hrpL activates Erwinia amylovora hrp gene transcription and is a member of the ECF subfamily of Σ factors.. J Bacteriol 177:6201–6210
    [Google Scholar]
  40. Willis D.K., Rich J.J., Hrabak E.M. 1991; hrpgenes of phytopathogenic bacteria.. Mol Plant-Microbe Interact 4:132–138
    [Google Scholar]
  41. Wilson M., Epton H. A. S., Sigee D. C. 1992a; Biological control of fire blight of hawthorn (Crataegus monogyna) with fluorescent Pseudomonas spp. under protected conditions.. Phytopathology 136:6–26
    [Google Scholar]
  42. Wilson M., Epton H. A. S., Sigee D. C. 1992b; Interactions between Erwinia herbicola and E. amylovora on the stigma of hawthorn blossoms.. Phytopathology 82:914–918
    [Google Scholar]
  43. Wrather J.A., Kuc J., Williams E.B. 1973; Protection of apple and pear fruit tissue against fireblight with nonpathogenic bacteria.. Phytopathology 63:1075–1076
    [Google Scholar]
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