1887

Abstract

is the causative agent of ovine footrot. The regions of the genome may have arisen by the integration of a genetic element and may have a role in virulence. The virulent strain A198 has multiple copies of the regions. In the present study, sequences to the left and right of regions 1, 2 and 3 of strain A198 were analysed by Southern blotting and DMA sequencing. The results suggest that regions 1 and 2 arose by independent integration events into different tRNA genes. The discovery of a second integrase gene (), a gene with similarity to bacteriophage repressor proteins (), and a gene similar to an ORF from a conjugative transposon (), suggests that a second genetic element, either a bacteriophage or a conjugative transposon, is integrated next to region 3 in the genome. The arrangement of and the regions in three other virulent strains and one benign strain was determined using using Southern blotting and PCR. One strain, H1215, contained and not and thus resembles region 3, suggesting that region 3 also may have arisen by an independent integration event. In all strains, a copy of was found next to the regions. The regions contain two genes, and with similarity to the genes of the killer plasmid Rts1. Evidence is presented that and have a similar function in

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-143-2-553
1997-02-01
2021-05-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/143/2/mic-143-2-553.html?itemId=/content/journal/micro/10.1099/00221287-143-2-553&mimeType=html&fmt=ahah

References

  1. Argos P., Landy A., Abremski K., Egan J.B., Haggard-Linquist E., Hoess R.H., Kahn M., Kalionis B., Narayan S.V.L., Pierson L.S. III Sternberg N., Leong J.M. 1986; The integrase family of site-specific recombinases: regional similarities and global diversity.. EMBO J 5:433–440
    [Google Scholar]
  2. Beveridge W.I.B. 1941; Footrot in sheep: a transmissible disease due to infection with Fusifortnis nodosus. . CS1RO Bull 140:1–40
    [Google Scholar]
  3. Billington S. J., Johnston J. L., Rood J. I. 1996a; Virulence regions and virulence factors of the ovine footrot pathogen, Dichelobacter nodosus. . FEMS Microbiol Lett 145:147–156
    [Google Scholar]
  4. Billington S. J., Sinistaj M., Cheetham B. F., Ayres A., Moses E. K., Katz M. E., Rood J. I. 1996b; Identification of a native Dichelobacter nodosus plasmid and implications for the evolution of the vap regions.. Gene 172:111–116
    [Google Scholar]
  5. Brennan R.G., Matthews B.W. 1989; The helix-turn-helix DNA binding motif.. J Biol Chem 264:1903–1906
    [Google Scholar]
  6. Calendar R., Ljungquist E., Deho G., Usher D.C., Youderian P., Sironi G., Six E.W. 1981; Lysogenization by satellite phage P4.. Virology 113:20–38
    [Google Scholar]
  7. Cheetham B.F., Tattersall D.B., Bloomfield G.A., Rood J.I., Katz M.E. 1995; Identification of a bacteriophage-related integrase gene in a vap region of the Dichelobacter nodosus genome.. Gene 162:53–58
    [Google Scholar]
  8. Cirillo J.D., Weisbrod T.R., Pascopella L., Bloom B.R., Jacobs W.R.J. 1994; Isolation and characterization of the asparto- kinase and aspartate semialdehyde dehydrogenase operon from mycobacteria.. Mol Microbiol 11:629–639
    [Google Scholar]
  9. Clark C.A., Beltrame J., Manning P.A. 1991; The oac gene encoding lipopolysaccharide O-antigen acetylase maps adjacent to the integrase-coding gene on the genome of Shigella flexneri bacteriophage Sf6.. Gene 107:43–52
    [Google Scholar]
  10. Clarke D.J., Dowds B.C. 1994; The gene coding for polynucleotide phosphorylase in Photorhabdus sp. strain K122 is induced at low temperatures.. J Bacteriol 176:3775–3784
    [Google Scholar]
  11. Claxton P.D., Ribeiro L.A., Egerton J.R. 1983; Classification of Bacteroides nodosus by agglutination tests.. Aust Vet J 60:331–334
    [Google Scholar]
  12. Crouzet J., Levy-Schil S., Cameron B., Cauchois L., Rigault S., Rouyez M.-C., Blanche F., Debussche L., Thibaut D. 1991; Nucleotide sequence and genetic analysis of a 13-1 kb Pseudomonas denitrificans DNA fragment containing five COB genes and identification of structural genes encoding COB(I) alamin adenosyltransferase, cobyric acid synthase and bifunctional cobin.. J Bacteriol 173:6074–6087
    [Google Scholar]
  13. Dorman C.J. 1994; The bacterial genome: plasmids.. In Genetics of Bacterial Virulence pp. 52–87 Oxford:: Blackwell.;
    [Google Scholar]
  14. Fleischmann R.D. 1995; Whole-genome random sequencing and assembly of Haemophilus influenzae Rd.. Science 269:496–512
    [Google Scholar]
  15. Gaeta B.A. 1996; Database similarity searching using blast and fasta. . Aust Biotechnol 5:282–290
    [Google Scholar]
  16. Grosjean H., Nicoghosian K., Baumont E., Soil D., Cedergren R. 1985; Nucleotide sequences of two serine tRNAs with a GGA anticodon: the structure-function relationships in the serine family of tRNAs.. Nucleic Acids Res 13:5697–5706
    [Google Scholar]
  17. Higgins D.G., Bleasby A.J., Fuchs R. 1992; Clustal V: improved software for multiple sequence alignment.. Comput Appl Biosci 8:189–191
    [Google Scholar]
  18. Katz M.E., Howarth P.M., Yong W.K., Riffkin G.G., Depiazzi L.J., Rood J.I. 1991; Identification of three gene regions associated with virulence in Dichelobacter nodosus, the causative agent of ovine footrot.. J Gen Microbiol 137:2117–2124
    [Google Scholar]
  19. Katz M.E., Strugnell R.A., Rood J.I. 1992; Molecular characterization of a genomic region associated with virulence in Dichelobacter nodosus. . Infect Immun 60:4586–4592
    [Google Scholar]
  20. Katz M.E., Wright GL, Cheetham B.F., Doidge C.V., Moses E.K., Rood J.I. 1994; Genetic organization of the duplicated vap region of the Dichelobacter nodosus genome.. J Bacteriol 176:2663–2669
    [Google Scholar]
  21. Kovach M.E., Peterson K.M. 1994; Identification of a Vibrio cholerae gene with homology to the integrase family of site- specific recombinases.. Infect Immun 62:3289–3298
    [Google Scholar]
  22. Krause M., Guiney D.G. 1991; Identification of a multimer resolution system involved in stabilization of the Salmonella dublin virulence plasmid pSDL2.. J Bacteriol 173:5754–5762
    [Google Scholar]
  23. Liu Y., Chatterjee A., Chatterjee A.K. 1995; Nucleotide sequence, organization, and expression of rdgA and rdgB genes that regulate pectin lyase production in the plant pathogenic bacterium Frwinina carotovora subsp.carotovora in response to DNA damage.. Mol Microbiol 14:999–1010
    [Google Scholar]
  24. Matsui H., Sano Y., Ishihara H., Shinomiya T. 1993; Regulation of pyocin genes in Pseudomonas aeruginosa by positive (prtN) and negative (prtR) regulatory genes.. J Bacteriol 175:1257–1263
    [Google Scholar]
  25. Ogawa T., Ogawa H., Tomizawa J. 1988; Organization of the early region of bacteriophage phi80. Genes and proteins.. J Mol Biol 202537–550
    [Google Scholar]
  26. Pearson W.R., Lipman D.J. 1988; Improved tools for biological sequence comparison.. Proc Natl Acad Sci USA 852444–2448
    [Google Scholar]
  27. Pullinger G.D., Lax A.J. 1992; A Salmonella dublin virulence plasmid locus that affects bacterial growth under nutrient-limited conditions.. Mol Microbiol 6:1631–1643
    [Google Scholar]
  28. Regnier P., Grunberg-Manago M., Portier C. 1987; Nucleotide sequence of the pnp gene of Escherichia coli encoding polynucleotide phosphorylase. Homology of the primary structure of the protein with the RNA-binding domain of ribosomal protein SI. J Biol Chem 262:63–68
    [Google Scholar]
  29. Rioux C.R., Friedrich M.J., Kadner R.J. 1990; Genes on the 90-kilobase plasmid of Salmonella typhimurium confer low- affinity cobalamin transport: relationship to fimbria biosynthesis genes.. J Bacteriol 172:6217–6222
    [Google Scholar]
  30. Rood J.I., Howart P.A., Haring V., Yong W.K., Liu D., Palmer M.A., Pitman D.R., Links I., Stewart D.J., Vaughan J.A. 1996; Comparison of gene probe and conventional methods for the diagnosis of ovine footrot.. Vet Microbiol 52:127–142
    [Google Scholar]
  31. Salyers A.A., Shoemaker N.B., Stevens A.M., Li L.-Y. 1995; Conjugative transposons: an unusual and diverse set of integrated gene transfer elements.. Microbiol Rev 59:579–590
    [Google Scholar]
  32. Shine J., Dalgarno L. 1974; The 3′ terminal sequence of E. coli 16S ribosomal RNA complementary to nonsense triplets and ribosome binding sites.. Proc Natl Acad Sci USA 711342–1346
    [Google Scholar]
  33. Stevens A.M., Shoemaker N.B., Li L.Y., Salyers A.A. 1993; Tetracycline regulation of genes on Bacteroides conjugative transposons.. J Bacteriol 175:6134–6141
    [Google Scholar]
  34. Sun J., Inouye M., Inouye S. 1991; Association of a retroelement with a P4-like cryptic prophage (retronphage phiR73) integrated into the selenocystyl tRNA gene of E.coli.. J Bacteriol 173:4171–4181
    [Google Scholar]
  35. Tian Q.B., Ohnishi M., Tabuchi A., Terawaki Y. 1996; A new plasmid-encoded killer gene system: cloning, sequencing, and analyzing big locus of plasmid Rtsl.. Biochem Biophys Res Commun 220:280–284
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-143-2-553
Loading
/content/journal/micro/10.1099/00221287-143-2-553
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error