1887

Abstract

The subsp. (formerly ) atypical insertion sequence, encodes a novel gene on the complementary strand to the putative transposase, p43. This gene requires a promoter, ribosome binding site (RBS) and termination codon to be acquired upon insertion into the subsp. genome and hence is designated the (ost xpression-ependent) gene of Analysis of insertion sites suggests that this element targets translation initiation signals in subsp. specifically inserting between the RBS and start codon of a putative gene sequence. This aligns the initiation codon adjacent to a functional RBS and possibly downstream of an active promoter, driving expression of Hed protein. We have confirmed this unique targeting process by detecting expression of in subsp. at the level of transcription by reverse transcription-PCR. Further, two Hed-specific antibodies detected Hed translation products in Western blots of protein extracts from subsp. A recombinant form of Hed expressed and purified from will facilitate studies of transposition and will also be assessed as a diagnostic antigen for subsp. disease. Implications of insertion in subsp. pathogenicity are discussed.

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1997-02-01
2021-04-22
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