1887

Abstract

Cell-free extracts of grown with thiosulfate as energy source and prepared at high ammonium sulfate concentrations and at low pH are capable of polythionate hydrolysis. The enzyme responsible for the hydrolysis of tetrathionate (SO ) and pentathionate (SO ) was purified to homogeneity. Enzyme activity during the purification procedure was based on a continuous spectrophotometric method that detects soluble intermediates that absorb in the UV region. The end products of hydrolysis of both polythionates by the pure enzyme were thiosulfate, sulfur and sulfate. The purified enzyme has a pH optimum of around 4 and a temperature optimum of 65 �. The activity is strongly influenced by the presence of sulfate ions. The purified enzyme is a dimer with two identical subunits of molecular mass 52 kDa. During purification of tetrathionate hydrolase, fractions able to hydrolyse trithionate and tetrathionate were separated, indicating that the two substrates are hydrolysed by different enzymes.

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1997-02-01
2021-07-23
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References

  1. Baer J.E., Carmack M. 1949; The ultraviolet absorption spectra of aliphatic sulfides and poly sulfides.. J Am Chem Soc 71:1215–1218
    [Google Scholar]
  2. Hazeu W., Bijleveld W., Grotenhuis J.T.C., Kakes E., Kuenen J.G. 1986; Kinetics and energetics of reduced sulfur oxidation by chemostat cultures of Thiobacillus ferrooxidans.. Antonie Leeuwenhoek 52:507–518
    [Google Scholar]
  3. Hazeu W., Batenburg-van der Vegte W.H., Bos P., van der Pas R.K., Kuenen J.G. 1988; The production and utilization of intermediary elemental sulfur during the oxidation of reduced sulfur compounds by Thiobacillus ferrooxidans.. Arch Microbiol 150:574–579
    [Google Scholar]
  4. Kelly D.P., Syrett P.J. 1966; [35S]Thiosulfate oxidation by Thiobacillus strain C.. Biochem J 98:537–545
    [Google Scholar]
  5. Kelly D.P., Chambers L.A., Trudinger P.A. 1969; Cyanolysis and spectrophotometric estimation of trithionate in mixtures with thiosulfate and tetrathionate.. Anal Chem 41:898–901
    [Google Scholar]
  6. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature 227:680–685
    [Google Scholar]
  7. Landesman J., Duncan D.W., Walden C.C. 1966; Oxidation of inorganic sulfur compounds by washed cell suspensions of Thiobacillus ferrooxidans.. Can J Microbiol 12:957–964
    [Google Scholar]
  8. Lane D.J., Harisson A.P., Stahl D., Pace B., Giovannoni S.J., Olsen G., Pace N.R. 1992; Evolutionary relationships among sulfur- and iron-oxidizing Eubacteria.. J Bacteriol 174:269–278
    [Google Scholar]
  9. London J., Rittenberg S.C. 1964; Path of sulfur in sulfide and thiosulfate oxidation by Thiobacilli.. Proc Natl Acad Sci USA 521183–1190
    [Google Scholar]
  10. Lu W.-P., Kelly D.P. 1988; Kinetic and energetic aspects of inorganic sulphur compound oxidation by Thiobacillus tepidarius.. J Gen Microbiol 134:865–876
    [Google Scholar]
  11. Meulenberg R., Pronk J.T., Hazeu W., Bos P., Kuenen J.G. 1992a; Oxidation of reduced sulphur compounds by intact cells of Thiobacillus acidophilus.. Arch Microbiol 157:161–168
    [Google Scholar]
  12. Meulenberg R., Pronk J.T., Frank J., Hazeu W., Bos P., Kuenen J.G. 1992b; Purification and partial characterization of a thermostable trithionate hydrolase from the acidophilic sulphur oxidizer Thiobacillus acidophilus.. Eur J Biochem 209:367–374
    [Google Scholar]
  13. Meulenberg R., Pronk J.T., Hazeu W., van Dijken J.P., Frank J., Bos P., Kuenen J.G. 1993a; Purification and partial characterization of thiosulphate dehydrogenase from Thiobacillus acidophilus.. J Gen Microbiol 139:2033–2039
    [Google Scholar]
  14. Meulenberg R., Scheer E.J., Pronk J.T., Hazeu W., Bos P., Kuenen J.G. 1993; . Metabolism of tetrathionate in Thiobacillus acidophilus.. FEMS Microbiol Lett 112:167–172
    [Google Scholar]
  15. Okuzumi M. 1965; Studies on biochemistry of the Thiobacilli. Part VII. Metabolism of tetrathionate by T. thiooxidans.. Agric Biol Chem 29:1069–1073
    [Google Scholar]
  16. Okuzumi M. 1966; Studies on biochemistry of the Thiobacilli. Part VIII. Dismutation of tetrathionate by T. thiooxidans.. Agric Biol Chem 30:313–318
    [Google Scholar]
  17. Okuzumi M., Kita Y. 1965; Studies on biochemistry of the Thiobacilli. Part VI. Oxidation of thiosulfate to tetrathionate by T. thiooxidans.. Agric Biol Chem 29:1063–1068
    [Google Scholar]
  18. Parker C.D., Prisk J. 1953; The oxidation of inorganic compounds of sulphur by various sulphur bacteria.. J Gen Microbiol 8:344–364
    [Google Scholar]
  19. Pronk J.T., Meulenberg R., Hazeu W., Bos P., Kuenen J.G. 1990; Oxidation of reduced inorganic sulphur compounds by acidophilic thiobacilli.. FEMS Microbiol Rev 75:293–309
    [Google Scholar]
  20. Sinha A.B., Walden C.C. 1966; Formation of polythionates and their interrelationships during oxidation of thiosulphate by Thiobacillus ferrooxidans.. Can J Microbiol 12:1041–1054
    [Google Scholar]
  21. Sklodowska A. 1988; Polythionates and adenosine-5-phospho-sulfate formation during thiosulfate oxidation by Thiobacillus neapolitanus.. Can J Microbiol 34:1283–1287
    [Google Scholar]
  22. Starkey R.L. 1935; Products of the oxidation of thiosulfate by bacteria in mineral media.. J Gen Physiol 18:325–349
    [Google Scholar]
  23. Steudel R. 1989; On the nature of the ̒elemental sulfur̓ (S°) produced by sulfur-oxidizing bacteria -a model for S° globules.. In Autotrophic Bacteria pp. 289–303 Schlegel H.G., Bowien S. Edited by Madison, USA:: Science Tech Publishers.;
    [Google Scholar]
  24. Steudel R., Holdt G., Göbel T., Hazeu W. 1987; Chromato-graphic separation of higher polythionates SnO6 2−(n = 3...22) and their detection in cultures of Thiobacillus ferrooxidans; molecular composition of bacterial sulfur secretions.. Angew Chem Int Ed Engl 26:151–153
    [Google Scholar]
  25. Steudel R., Göbel T., Holdt G. 1988; The molecular composition of hydrolytic sulfur sols prepared by acid decompo-sition of thiosulfate.. Z Naturforsch 43b:203–218
    [Google Scholar]
  26. Trudinger P.A. 1964a; Evidence for a four-sulphur intermediate in thiosulfate oxidation by Thiobacillus X.. Aust J Biol Sci 17:577–579
    [Google Scholar]
  27. Trudinger P.A. 1964b; The metabolism of trithionate by Thiobacillus X.. Aust J Biol Sci 17:459–468
    [Google Scholar]
  28. Trudinger P.A. 1964c; Products of anaerobic metabolism of tetrathionate by Thiobacillus X.. Aust J Biol Sci 17:446–458
    [Google Scholar]
  29. Trudinger P.A. 1965; Effect of thiol-binding reagents on the metabolism of thiosulfate and tetrathionate.. J Bacteriol 89:617–625
    [Google Scholar]
  30. Van lersel J., Frank Jzn J., Duine J.A. 1985; Determination of absorption coefficients of purified proteins by conventional ultraviolet spectrophotometry and chromatography combined with multiwavelength detection.. Anal Biochem 151:196–204
    [Google Scholar]
  31. Vishniac W. 1952; The metabolism of Thiobacillus thioparus. I. The oxidation of thiosulfate.. J Bacteriol 64:363–373
    [Google Scholar]
  32. Wood A.P., Kelly D.P. 1986; Chemolithotrophic metabolism of the newly-isolated moderately thermophilic, obligately autotrophic Thiobacillus tepidarius.. Arch Microbiol 144:71–77
    [Google Scholar]
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