1887

Abstract

Reported differences in antibiotic production dynamics resulting from altering the growth-limiting nutrient (growth-dissociated production in carbon-limited culture and apparent growth-associated production in nitrogen-limited culture) are due to the different effects on growth kinetics. The substrate affinity for nitrate is significantly lower than that for glucose, resulting in nitrogen limitation effectively occurring throughout the culture. Glucose limitation occurs later in the culture, coinciding with the induction of antibiotic production. Induction occurs at the start of nitrogen-limited culture so that production appears to be growth-associated. Evidence that this hypothesis is consistent with production kinetics in cyclic fed batch culture was also obtained.

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1997-02-01
2024-04-19
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References

  1. Aharonowitz Y. 1980; Nitrogen metabolite regulation of antibiotic biosynthesis.. Annu Rev Microbiol 34:209–233
    [Google Scholar]
  2. Bascaran V., Sanchez L., Hardisson C., Brana A.F. 1991; Stringent response and initiation of secondary metabolism in Streptomyces clavuligerus.. J Gen Microbiol 137:1625–1634
    [Google Scholar]
  3. Beppu T. 1992; Secondary metabolites as chemical signals for cellular differentiation.. Gene 115:159–165
    [Google Scholar]
  4. Bibb M.J. 1996; The regulation of antibiotic production in Streptomyces coelicolor A3(2).. Microbiology 142:1335–1344
    [Google Scholar]
  5. Bree M.A., Dhurjati P., Georghegan R.F., Robnett B. 1988; Kinetic modelling of hybridoma cell growth and immunoglobulin production in a large-scale suspension culture.. Biotechnol Bioeng 32:1067–1072
    [Google Scholar]
  6. Bushell M.E., Bell S.L., Scott M.F., Snell K., Speir R.E., Wardell J.N., Sanders P.G. 1993; A three-phase pattern in growth, monoclonal antibody production and metabolite exchange in a hybridoma bioreactor culture.. Biotechnol Bioeng 42:133–139
    [Google Scholar]
  7. Chater K.F., Bibb M.J. 1996; Regulation of bacterial antibiotic production.. In Products of Secondary Metabolism (Biotechnology vol. 6) Edited by Kleinkauf H., von Dohren H. Weinheim: VCH (in press);
    [Google Scholar]
  8. Clark G.J., Langley D., Bushell M.E. 1995; Oxygen limitation can induce microbial secondary metabolite formation: investigations with miniature electrodes in shaker and bioreactor culture.. Microbiology 141:663–669
    [Google Scholar]
  9. Demain A.L., Aharonowitz Y., Martin J.-F. 1983; Metabolic control of secondary biosynthetic pathways.. In Biochemistry and Genetic Regulation of Commercially Important Antibiotics pp. 49–72 Edited by Vining L. C. Reading, MA: Addison-Wesley;
    [Google Scholar]
  10. Granozzi C., Billetta R., Passantino R., Sallazo M., Puglia A. M. 1990; A breakdown in macromolecular synthesis preceding differentiation in Streptomyces coelicolor (A3)2.. J Antibiot 29:713–716
    [Google Scholar]
  11. Huck T.A., Porter N., Bushell M.E. 1991; Positive selection of antibiotic-producing soil isolates.. J Gen Microbiol 137:2321–2329
    [Google Scholar]
  12. Koch A.L. 1980; The inefficiency of ribosomes functioning in Escherichia coli growing at moderate rates.. J Gen Microbiol 116:165–171
    [Google Scholar]
  13. Lamond A.1., Travers A.A. 1985; Stringent control of bacterial transcription.. Cell 41:313–334
    [Google Scholar]
  14. Lynch H.C., Bushell M.E. 1995; The physiology of erythromycin biosynthesis in cyclic fed batch culture.. Microbiology 141:3105–3111
    [Google Scholar]
  15. McDermott J.F., Lethbridge G., Bushell M.E. 1993; Estimation of the kinetic constants and elucidation of trends in growth and erythromycin production in batch and continuous cultures of Saccharopolyspora erythraea using curve fitting techniques.. Enzyme Microb Technol 15:657–663
    [Google Scholar]
  16. Martin J.F. 1976; Phosphate regulation of gene expression in candidicin biosynthesis.. In Microbiology - 1976 pp. 548–552 Edited by Schlessinger D. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  17. Martin J.F., Demain A.L. 1978; Control of antibiotic biosynthesis.. Microbiol Rev 44:230–251
    [Google Scholar]
  18. Ochi K. 1988; Nucleotide pools and stringent response in regulation of Streptomyces differentiation.. In Proceedings of the 7th International Symposium on the Biology of Actinomycetes pp. 330–337 Edited by Okami Y., Beppu T., Ogawara H. Tokyo: Japan Scientific Societies Press;
    [Google Scholar]
  19. Oner M.D., Erickson L. E., Young S. S. 1986; Utilisation of spline functions for smoothing fermentation data and for estimation of specific rates Biotechnol Bioeng, . 28:902–918
    [Google Scholar]
  20. Roth J., Leroith D., Collier E.S., Watkinson A., Lesniak M.A. 1986; The evolutionary origins of intercellular communication and the Maginot Lines of the mind.. Ann NY Acad Sci 463:1–11
    [Google Scholar]
  21. Takano E., Gramajo H.C., Stauch E., Andres N., White J., Bibb M.J. 1992; The stringent response in Streptomyces coelicolor A3 (2).. Mol Microbiol 6:2797–2804
    [Google Scholar]
  22. Tsuji K., Goetz J.F. 1978; High performance liquid chromatographic determination of erythromycin.. J Chromatogr 147:359–367
    [Google Scholar]
  23. Wilson G.C., Bushell M.E. 1995; The induction of antibiotic synthesis in Saccharopolyspora erythraea and Streptomyces hygroscopicus by growth rate decrease is accompanied by a down-regulation of protein synthesis rate.. FEMS Microbiol Lett 129:89–96
    [Google Scholar]
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