1887

Abstract

We report here the cloning of the genomic topoisomerase I gene () by use of PCR and subsequent hybridization. The predicted protein sequence shared 58.8% identity with the topoisomerase I and 30-50% identity with other eukaryotic topoisomerase I proteins. A conditional gene disruption strain (CWJ477) was constructed so that one copy of was deleted and the other copy of was placed under a regulatable promoter. Under repressed conditions, cells grew slowly and cell morphology was abnormal. The virulence of CWJ477 was markedly reduced in a mouse model system, and that of the single gene knockout strain was slightly attenuated, indicating that might play a role in the infection of in mice in a dose-dependent manner. Despite the reduced virulence of both the single and double knockout strains, viable cells of the pathogen were recovered from the kidneys as late as 22 d post-infection.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-143-2-377
1997-02-01
2019-10-15
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-143-2-377
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error