1887

Abstract

Screening cDNA libraries with polyclonal antibody preparations against yeast or mycelial cell walls resulted in isolation of several positive clones. Some of them encoded enolase; others encoded a protein of the 70 kDa heat-shock protein family (Hsp70p), etc. The presence of these cytosolic proteins in the cell wall of actively growing was discovered by analytical (SDS-PAGE and Western blot) and cytological (indirect immunofluorescence) experiments. Supplementation of cell cultures with papulacandin B, an antibiotic that inhibits formation of the -glucan skeleton, resulted in the release of enolase to the supernatant fluids; this release was prevented when 0.6 M KCI was present as an osmotic stabilizer. The cell wall of incorporated exogenously added proteins (enolase and and cytosolic proteins). The presence in the cell wall of enolase, Hsp70p, and probably other intracellular proteins that are highly immunogenic might help the fungal cells to evade the host defences, and consequently could represent a survival mechanism for

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-143-2-313
1997-02-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/143/2/mic-143-2-313.html?itemId=/content/journal/micro/10.1099/00221287-143-2-313&mimeType=html&fmt=ahah

References

  1. Alaei S., Larcher C., Ebenbichler C., Prodinger W.M., Janatova J., Dierich M.P. 1993; Isolation and biochemical characterization of the iC3b receptor of Candida albicans. . Infect Immun 61:1395–1399
    [Google Scholar]
  2. Anders R.F., McColl D.J., Coppel R.L. 1993; Molecular variation in Plasmodium falciparum: polymorphic antigens of asexual erythrocytic stages.. Acta Trop 53:239–253
    [Google Scholar]
  3. Angiolella L., Facchin M., Stringaro A., Maras B., Simonetti N., Cassone A. 1996; Identification of a glucan-associated enolase as a main cell wall protein of Candida albicans and an indirect target of lipopeptide antimycotics.. J Infect Dis 173:684–690
    [Google Scholar]
  4. Baguley B.C, Rümmele G.J., Wehrli W. 1979; Papulacandin B: an inhibitor of glucan synthesis in yeast spheroplasts.. Eur J Biochem 97:345–351
    [Google Scholar]
  5. Bianco A. E., Favaloro J. M., Burkot T. R., Culvenor J. G., Crewther P. E., Brown G. V., Anders R. F., Coppel R. L., Kemp D.J. 1986; A repetitive antigen of Plasmodium falciparum that is homologous to heat shock protein 70 of Drosophila melanogaster. . Proc Natl Acad Sci USA 838713–8717
    [Google Scholar]
  6. Boorstein W.R., Ziegelhoffer T., Craig E.A. 1994; Molecular evolution of the Hsp70 multigene family.. J Mol Evol 38:1–17
    [Google Scholar]
  7. Bouali A., Robert R., Tronchin G., Senet J.M. 1987; Characterization of binding of human fibrinogen to the surface of germ-tubes and mycelium of Candida albicans. . J Gen Microbiol 133:545–551
    [Google Scholar]
  8. Bouchara J.P., Tronchin G., Annaix V., Robert R., Senet J.M. 1990; Laminin receptors on Candida albicans germ tubes.. Infect Immun 58:48–54
    [Google Scholar]
  9. Bromuro C., Torosantucci A., Gomez M.J., Urbani F., Cassone A. 1994; Differential release of an immunodominant 65 kDa mannoprotein antigen from yeast and mycelial form of Candida albicans. . J Med Vet Mycol 32:447–459
    [Google Scholar]
  10. Burnette W.N. 1981; ‘Western blotting’: electrophoretic transfer of proteins from sodium dodecyl sulphate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein. A. Anal Biochem 112:195–203
    [Google Scholar]
  11. Calderone R., Fukayama M. 1992; Virulence-associated mannoproteins of Candida albicans. . In Emerging Targets in Antibacterial and Antifungal Chemotherapy pp. 524–545 Sutcliffe J.A., Georgepapadakon N. H. Edited by New York:: Chapman and Hall.;
    [Google Scholar]
  12. Calderone R.A., Linehan L., Wadsworth E., Sandberg A.L. 1988; Identification of C3d receptors on Candida albicans. . Infect Immun 56:252–258
    [Google Scholar]
  13. Casanova M., Gil M.L., Cardeñoso L., Martinez J.P., Sentandreu R. 1989; Identification of wall-specific antigens synthesized during germ tube formation by Candida albicans. . Infect Immun 57:262–271
    [Google Scholar]
  14. Constantino P.J., Franklyn K.M., Gare N.F., Warmington J.R. 1994; Production of antibodies to antigens of Candida albicans in CBA/H mice.. Infect Immun 62:1400–1405
    [Google Scholar]
  15. Elorza M.V., Murgui A., Sentandreu R. 1985; Dimorphism in Candida albicans: contribution of mannoproteins to the architecture of yeast and mycelial cell walls.. J Gen Microbiol 131:2209–2216
    [Google Scholar]
  16. Elorza M.V., Marcilla A., Sentandreu R. 1988; Wall mannoproteins of the yeast and mycelial cells of Candida albicans: nature of the glycosidic bonds and polydispersity of their mannan moieties.. Gen Microbiol 134:2393–2403
    [Google Scholar]
  17. Eroles P., Sentandreu M., Elorza M.V., Sentandreu R. 1995; Cloning of a DNA fragment encoding part of a 70-kDa heat shock protein of Candida albicans. . FEMS Microbiol Lett 128:95–100
    [Google Scholar]
  18. Felger I., Tavui L., Kabintik S., Marshall V., Genton B., Alpers M., Beck H. P. 1994; Plasmodium falciparum: extensive polymorphism in merozoite surface antigen 2 alleles in an area with endemic malaria in Papua New Guinea.. Exp Parasitol 79:106–116
    [Google Scholar]
  19. Font de Mora J., Herrero E., Sentandreu R. 1993; A kinetic study on the regeneration of Candida albicans protoplasts in the presence of cell wall synthesis inhibitors.. FEMS Microbiol Lett 111:43–48
    [Google Scholar]
  20. Franklyn K.M., Warmington J.R., Ott A.K., Ashman R.B. 1990; An immunodominant antigen of Candida albicans shows homology to the enzyme enolase.. Immunol Cell Biol 68:173–178
    [Google Scholar]
  21. Frontali C. 1994; Genome plasticity in Plasmodium.. Genetica 94:91–100
    [Google Scholar]
  22. Glee P., Sundstrom M., Hazen K.C. 1995; Expression of surface hydrophobic proteins by Candida albicans in vivo.. Infect Immun 63:1373–1379
    [Google Scholar]
  23. Gomez M.J., Torosantucci A., Quinti I., Testa U., Peschle C., Cassone A. 1993; Mannoprotein-induced anti-U937 cell cytotoxicity in peripheral blood mononuclear cells from uninfected or HIV-infected subjects: role of interferon-gamma and tumor necrosis factor a cells.. Infect Immun 152:530–543
    [Google Scholar]
  24. Gustafson K.S., Vercelloti G.M., Bendel C.M., Hostetter M.K. 1991; Molecular mimicry in Candida albicans. Role of an integrin analogue in adhesion of yeast to human endothelium.. J Clin Invest 87:1896–1902
    [Google Scholar]
  25. Hazen K.C., Glee P.M. 1994; Hydrophobic cell wall protein glycosylation by the pathogenic fungus Candida albicans. . Can J Microbiol 40:266–272
    [Google Scholar]
  26. Kalon A., Segal E., Shar E., Dayan D. 1988; Interaction of Candida albicans with genital humoral surfaces: involvement of fibronectin in adherence.. J Infect Dis 57:1253–1256
    [Google Scholar]
  27. Kapteyn J.C., Montijn R.C., Dijkgraaf G.J.P., Klis F.M. 1994; Identification of β-1,6-glucosylated cell wall proteins in yeast and hyphal forms of Candida albicans. . Eur J Cell Biol 65:402–407
    [Google Scholar]
  28. Kapteyn J.C., Dijkgraaf G.J.P., Montijn R.C.8cKlis. 1995; Glycosylation of cell wall proteins in regeneration spheroplasts of Candida albicans. . FEMS Microbiol Lett 128:271–277
    [Google Scholar]
  29. Klotz S.A. 1990; Adherence of Candida albicans to components of the subendothelial extracellular matrix.. FEMS Microbiol Lett 68:249–254
    [Google Scholar]
  30. Klotz S.A., Maca R.D. 1988; Endothelial cell contraction increases Candida adherence to exposed extracellular matrix.. Infect Immun 56:2495–2498
    [Google Scholar]
  31. Klotz S.A., Smith R.L. 1991; A fibronectin receptor on Candida albicans mediates adherence of the fungus to extracellular matrix.. J Infect Dis 163:604–610
    [Google Scholar]
  32. Klotz S.A., Hein R.C., Smith R.L., Rouse J.B. 1994; The fibronectin adhesin of Candida albicans. . Infect Immun 62:4679–4681
    [Google Scholar]
  33. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature 227:680–685
    [Google Scholar]
  34. La Valle R., Bromuro G, Ranucci L., Muller H.-M., Crisanti A., Cassone A. 1995; Molecular cloning and expression of a 70- kilodalton heat shock protein of Candida albicans. . Infect Immun 63:4039–4045
    [Google Scholar]
  35. Lee K.L, Buckley M.R., Campbell C. 1975; An amino acid liquid synthetic medium for development of mycelial and yeast forms of Candida albicans. . Sabouraudia 13:148–153
    [Google Scholar]
  36. López-Ribot J.L., Chaffin W.L. 1994; Binding of the extracellular matrix component entactin to Candida albicans. . Infect Immun 62:4564–4571
    [Google Scholar]
  37. López-Ribot J.L., Casanova M., Monteagudo C., Sepulveda P., Martinez J.P. 1994; Evidence for the presence of a high- affinity laminin receptor-like molecule on the surface of Candida albicans yeast cells.. Infect Immun 62:742–746
    [Google Scholar]
  38. López-Ribot J.L., Alloush H.M., Masten B.J., Chaffin W.L. 1996; Evidence for presence in the cell wall of Candida albicans of a protein related to the hsp70 family.. Infect Immun 64:3333–3340
    [Google Scholar]
  39. McColl D.J., Silva A., Foley M., Kun J.F., Favaloro J.M., Thompson J.K., Marchall V.M., Coppel R.L., Kemp D.J., Anders R.F. 1994; Molecular variation in a novel polymorphic antigen associated with Plasmodium falciparum merozoites.. Mol Biochem Parasitol 68:53–67
    [Google Scholar]
  40. Marshall V.M., Anthony R.L., Bangs M.J., Purnomo R.F., Anders R.F., Coppel R.L. 1994; Allelic variants of the Plasmodium falciparum merozoite surface antigen 2 (MSA-2) in a geographically restricted area of Irian Jaya.. Mol Biochem Parasitol 63:13–21
    [Google Scholar]
  41. Martinez J.P., López-Ribot J.L., Chaffin L.J. 1994; Heterogeneous surface distribution of the fibrinogen-binding protein on Candida albicans. . Infect Immun 62:709–712
    [Google Scholar]
  42. Matthews R., Burnie J. 1989; Cloning of a DNA sequence encoding a major fragment of the 47 kilodalton stress protein homologue of Candida albicans. . FEMS Microbiol Lett 51:25–30
    [Google Scholar]
  43. Nelson R.D., Shibata N., Podzorski R.P., Herron M.J. 1991; Candida mannan: chemistry, suppression of cell-mediated immunity, and possible mechanisms of action.. Clin Microbiol Rev 4:1–19
    [Google Scholar]
  44. Pelham H.R.B. 1986; Speculations on the functions of the major heat shock and glucose-related proteins.. Cell 46:959–961
    [Google Scholar]
  45. Postlethwait P., Sundstrom P. 1995; Genetic organization and mRNA expression of enolase genes of Candida albicans. . J Bacteriol 177:1772–1779
    [Google Scholar]
  46. Sanjuán R., Zueco J., Stock R., Font de Mora J., Sentandreu R. 1995; Identification of glucan-mannoprotein complexes in the cell wall of Candida albicans using a monoclonal antibody that reacts with a (l,6)-β-glucan epitope.. Microbiology 141:1545–1551
    [Google Scholar]
  47. Sentandreu R., Mormeneo S., Ruiz-Herrera J. 1994; Biogenesis of the fungal cell wall.. In The Mycota. I. Growth, Differentiation and Sexuality pp. 111–124 Wessels J. G. H., Meinhart F. Edited by Berlin & Heildelberg:: Springer-Verlag.;
    [Google Scholar]
  48. Sentandreu M., Elorza M.V., Sentandreu R., Gozalbo D. 1995; Cloning of cDNAs coding for Candida albicans cell surface proteins.. J Med Vet Mycol 33:105–111
    [Google Scholar]
  49. Shepherd M.G., Poulter R.T.M., Sullivan P.A. 1985; Candida albicans: biology, genetics and pathogenicity.. Annu Rev Microbiol 39:579–614
    [Google Scholar]
  50. Sundstrom P., Aliaga G.R. 1992; Molecular cloning of cDNA and analysis of protein secondary structure of Candida albicans enolase, an abundant, immunodominant glycolytic enzyme.. J Bacteriol 174:6789–6799
    [Google Scholar]
  51. Sundstrom P., Aliaga G.R. 1994; A subset of proteins found in culture supernatants of Candida albicans includes the abundant, immunodominant, glycolytic enzyme enolase.. J Infect Dis 169:452–456
    [Google Scholar]
  52. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.. Proc Natl Acad Sci USA 764350–4354
    [Google Scholar]
  53. Walsh T.J., Hathorn J.W., Sobel J.D. 12 other authors 1991; Detection of circulating Candida enolase by immunoassay in patients with cancer and invasive candidiasis.. N Engl J Med 324:1026–1031
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-143-2-313
Loading
/content/journal/micro/10.1099/00221287-143-2-313
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error