%0 Journal Article %A Magee, B. B. %A Magee, P. T. %T WO-2, a stable aneuploid derivative of Candida albicans strain WO-1, can switch from white to opaque and form hyphae %D 1997 %J Microbiology, %V 143 %N 2 %P 289-295 %@ 1465-2080 %R https://doi.org/10.1099/00221287-143-2-289 %K HIS1 %K Candida albicans %K LEU2 %K chromosome loss %K aneuploidy %I Microbiology Society, %X Candida albicans strain WO-2 was isolated as a spontaneous derivative of the white-opaque switching strain WO-1. The electrophoretic karyotype of WO-2 lacks two bands which are found in the parent. These bands correspond to one homologue of chromosome 7 and to a translocation product containing parts of chromosomes 6 and 5. Probing a blot of the karyotype demonstrated that the genetic material in these bands had been lost, yielding an aneuploid strain. UV-irradiation experiments showed that auxotrophs due to mutation in genes located in this region predominated, supporting the conclusion that WO-2 is partially haploid. WO-2 contained about 10% of its genome in the haploid state, and it grew with a doubling time of about twice that of its parent. However, it was able to undergo both the yeast-to-hyphal transition and the white-opaque transition. Hence, these processes do not require perfect diploidy. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-143-2-289