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Microbiology Society logo Microbiology

Volume 143, Issue 12

The haemagglutinin of type C progenitor toxin plays an essential role in binding of toxin to the epithelial cells of guinea pig small intestine, leading to the efficient absorption of the toxin Free

Abstract

Binding of the purified type C 7S (neurotoxin), 12S and 16S botulinum toxins to epithelial cells of ligated small intestine or colon of the guinea pig ( test) and to pre-fixed gastrointestinal tissue sections ( test) was analysed. The 16S toxin bound intensely to the microvilli of epithelial cells of the small intestine in both and tests, but did not bind to cells of the stomach or colon. The neurotoxin and 12S toxin did not bind to epithelial cells of the small intestine or to cells of the stomach or colon. Absorption of the toxins was assessed by determining the toxin titre in the sera of guinea pigs 6-8 h after the intra-intestinal administration of the toxins. When the 16S toxin [1 x 10 minimum lethal dose (MLD)] was injected, 200-660 MLD ml was detected in the sera, whereas when the 12S toxin (2 x 10 MLD) or 7S toxin (2 x 10 MLD) was injected, little toxin activity was detected in the sera. Therefore, the haemagglutinin of type C 16S toxin is apparently very important in the binding and absorption of botulinum toxin in the small intestine.

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Keyword(s): absorption , Clostridium botulinum , haemagglutinin and toxin binding
Society For General Microbiology 1997
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1997-12-01
2025-03-15
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References

  1. Caldero J., Campo E., Calomarde X., Torra M. 1988; Distribution and changes in glycoconjugates in rat colonic mucosa during development.. Histochemistry 90:261–270
     [Google Scholar]
  2. Fujinaga Y., Inoue K., Shimazaki S., Tomochika K., Tsuzuki K., Fujii N., Watanabe T., Ohyama T., Takeshi K., Inoue K., Oguma K. 1994; Molecular construction of Clostridium botulinum type C progenitor toxin and its gene organization.. Biochem Biophys Res Commun 205:1291–1298
     [Google Scholar]
  3. Fujita R., Fujinaga Y., Inoue K., Nakajima H., Kumon H., Oguma K. 1995; Molecular characterization of two forms of nontoxic-nonhemagglutinin components of Clostridium botulinum type A progenitor toxins.. FEBS Lett 376:41–44
     [Google Scholar]
  4. Hauser D., Eklund M.W., Boquet P., Popoff M.R. 1994; Organisation of the botulinum neurotoxin Cl gene and its associated non-toxic protein genes in Clostridium botulinum C468.. Mol Gen Genet 243:631–640
     [Google Scholar]
  5. Henderson I., Whelan S.M., Davis T.O., Minton N.P. 1996; Genetic characterisation of the botulinum toxin complex of Clostridium botulinum strain NCTC 2916.. FEMS Microbiol Lett 140:151–158
     [Google Scholar]
  6. Inoue K., Fujinaga Y., Watanabe T., Ohyama T., Takeshi K., Moriishi K., Nakajima H., Inoue K., Oguma K. 1996; Molecular composition of Clostridium botulinum type A progenitor toxins.. Infect Immun 64:1589–1594
     [Google Scholar]
  7. Kimura K., Fujii N., Tsuzuki K., Murakami T., Indoh T., Yokosawa N., Takeshi K., Syuto B., Oguma K. 1990; The complete nucleotide sequence of the gene coding for botulinum type Cl toxin in the C-ST phage genome.. Biochem Biophys Res Commun 171:1304–1311
     [Google Scholar]
  8. Minton N.P. 1995; Molecular genetics of clostridial neurotoxins.. Curr Top Microbiol Immunol 95:161–194
     [Google Scholar]
  9. Oguma K., Syuto B., lida H., Kubo S. 1980; Antigenic similarity of toxins produced by Clostridium botulinum type C and D strains.. Infect Immun 30:656–660
     [Google Scholar]
  10. Oguma K., Fujinaga Y., Inoue K. 1995; Structure and function of Clostridium botulinum toxins.. Microbiol Immunol 39:161–168
     [Google Scholar]
  11. Ohishi I., Sakaguchi G. 1980; Oral toxicides of Clostridium botulinum type C and D toxins of different molecular sizes.. Infect Immun 28:303–309
     [Google Scholar]
  12. Ohyama T., Watanabe T., Fujinaga Y., Inoue K., Sunagawa H., Fujii N., Inoue K., Oguma K. 1995; Characterization of nontoxic-nonhemagglutinin component of two types of progenitor toxin (M and L) produced by Clostridium botulinum type D CB-16.. Microbiol Immunol 39:457–465
     [Google Scholar]
  13. Sugii S., Ohishi I., Sakaguchi G. 1977a; Correlation between oral toxicity and in vitro stability of Clostridium botulinum type A and B toxins of different molecular sizes.. Infect Immun 16:910–914
     [Google Scholar]
  14. Sugii S., Ohishi I., Sakaguchi G. 1977b; Intestinal absorption of botulinum toxins of different molecular sizes in rats.. Infect Immun 17:491–496
     [Google Scholar]
  15. Tsuzuki K., Yokosawa N., Syuto B., Ohishi I., Fujii N., Kimura K., Oguma K. 1988; Establishment of a monoclonal antibody recognizing an antigenic site common to Clostridium botulinum type B, Cl, D, and E toxins and tetanus toxin.. Infect Immun 56:868–902
     [Google Scholar]
  16. Tsuzuki K., Kimura K., Fujii N., Yokosawa N., Indoh T., Murakami T., Oguma K. 1990; Cloning and complete nucleotide sequence of the gene for the main component of hemagglutinin produced by Clostridium botulinum type C.. Infect Immun 58:3173–3177
     [Google Scholar]
  17. Tsuzuki K., Kimura K., Fujii N., Yokosawa N., Oguma K. 1992; The complete nucleotide sequence of the gene coding for the nontoxic-nonhemagglutinin component of Clostridium botu-linum type C progenitor toxin.. Biochem Biophys Res Commun 183:1273–1279
     [Google Scholar]
  18. Yoshimura S., Komatsu N., Watanabe K. 1980; Purification and immunohistochemical localization of rat liver glutathione peroxidase.. Biochim Biophys Acta 621:130–137
     [Google Scholar]
/content/journal/micro/10.1099/00221287-143-12-3841
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The haemagglutinin of Clostridium botulinum type C progenitor toxin plays an essential role in binding of toxin to the epithelial cells of guinea pig small intestine, leading to the efficient absorption of the toxin
Microbiology 143, 3841 (1997); https://doi.org/10.1099/00221287-143-12-3841
/content/journal/micro/10.1099/00221287-143-12-3841
/content/journal/micro/10.1099/00221287-143-12-3841
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