1887

Abstract

Since has emerged as one of the most common pathogens, DNA probes have been developed which fulfil the requirements for effective fingerprinting. Using a screen for complex genomic fragments containing moderately repetitive sequences, seven DNA probes were cloned which generate complex Southern blot hybridization patterns with -digested DNA. All of the probes are species-specific and the majority cross-hybridize to varying degrees. The capacity of two of the probes, Cg6 and Cg12, to measure genetic distance between independent isolates is verified by comparing clustering in dendrograms based on similarity coefficients computed between all pairs of 39 independent isolates fingerprinted with Cg6, Cg12 and randomly amplified polymorphic DNA. The capacity of the probes Cg6 and Cg12 to assess microevolution in clonal populations of infecting over time is also demonstrated. These probes can now be used in large computer-assisted epidemiological studies.

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1997-12-01
2021-05-08
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References

  1. Asakura K., Iwaguchi S., Homma M., Sukai T., Higashide K., Tanaka K. 1991; Electrophoretic karyotypes of clinically isolated yeasts of Candida albicans and C. glabrata. . J Gen Microbiol 25:31–38
    [Google Scholar]
  2. Beck-Sague C., Jarvis W.R. 1993; Secular trends in the epidemiology of nosocomial fungal infections in the United States, 1980-1990. National Nosocomial Infections Surveillance System.. J Infect Dis 167:1247–1251
    [Google Scholar]
  3. Burgener-Kairuz P., Zuber J.P., Jaunin P., Buchman T.G., Bille J., Rossier M. 1994; Rapid detection and identification of Candida albicans and Torulopsis (Candida) glabrata in clinical specimens by species-specific nested PCR amplification of a cytochrome P-450 lanosterol-alpha-demethylase (L1A1) gene fragment.. J Clin Microbiol 32:1902–1907
    [Google Scholar]
  4. Church G.M., Gilbert W. 1984; Genomic sequencing.. Proc Natl Acad Sci USA 811991–1995
    [Google Scholar]
  5. Clemons K.V., Feroze F., Holmberg K., Stevens D.A. 1997; Comparative analysis of genetic variability among Candida albicans isolates from different geographic locales by three genotypic methods.. J Clin Microbiol 35:1332–1336
    [Google Scholar]
  6. Girardin H., Latge J.P., Srikantha T., Morrow B., Soll D.R. 1993; Development of DNA probes for fingerprinting Aspergillus fumigatus. . J Clin Microbiol 31:1547–1554
    [Google Scholar]
  7. Hazen K.C. 1995; New and emerging yeast pathogens.. Clin Microbiol Rev 8:462–478
    [Google Scholar]
  8. Hellstein J., Vawter H.H., Fotos P., Schmid J., Soll D.R. 1993; Genetic similarity and phenotypic diversity of commensal and pathogenic strains of Candida albicans isolated from the oral cavity.. J Clin Microbiol 31:3190–3199
    [Google Scholar]
  9. Joly S., Pujol C., Schröppel K., Soll D.R. 1996; Development of two species-specific fingerprinting probes for broad computer- assisted epidemiological studies of Candida tropicalis. . J Clin Microbiol 34:3063–3071
    [Google Scholar]
  10. Jordan J.A. 1994; PCR identification of four medically important Candida species by using a single primer pair.. J Clin Microbiol 32:2962–2967
    [Google Scholar]
  11. Kaufmann C.S., Merz W.G. 1989; Electrophoretic karyotypes of Torulopsis glabrata. . J Clin Microbiol 27:2165–2168
    [Google Scholar]
  12. Khattak M.N., Burnie J.P., Matthews R.C., Oppenheim B.A. 1992; Clamped homogeneous electric field gel electrophoresis typing of Torulopsis glabrata isolates causing nosocomial infections.. J Clin Microbiol 30:2211–2215
    [Google Scholar]
  13. Lehmann P.F., Lin D., Lasker B.A. 1992; Genotypic identification and characterization of species and strains within the genus Candida by using random amplified polymorphic DNA.. J Clin Microbiol 30:3249–3254
    [Google Scholar]
  14. Lockhart S.R., Fritch J.J., Meier A.S., Schröppel K., Srikantha S., Galask R., Soll D.R. 1995; Colonizing populations of Candida albicans are clonal in origin but undergo microevolution through Cl fragment reorganization as demonstrated by DNA fingerprinting and Cl sequencing.. J Clin Microbiol 33:1501–1509
    [Google Scholar]
  15. Lockhart S.R., Reed B., Pierson C., Soll D.R. 1996; The most frequent scenario for recurrent Candida vaginitis is strain maintenance with ‘substrain shuffling’: demonstrated by sequential DNA fingerprinting with probes Ca3, Cl, and CARE2.. J Clin Microbiol 34:767–777
    [Google Scholar]
  16. Maiwald M., Kappe R., Sonntag H.G. 1994; Rapid presumptive identification of medically relevant yeasts to the species level by polymerase chain reaction and restriction enzyme analysis.. J Med Vet Mycol 32:115–122
    [Google Scholar]
  17. Mason M.M., Lasker B.A., Riggsby W.S. 1987; Molecular probe for identification of medically important Candida species and Torulopsis glabrata. . J Clin Microbiol 25:563–566
    [Google Scholar]
  18. Nguyen M.H., Peacock J.J., Morris A.J., Tanner D.C., Nguyen M.L., Snydman D.R., Wagener M.M., Rinaldi M.G., Yu V.L. 1996; The changing face of candidemia: emergence of non- Candida albicans species and antifungal resistance.. Am J Med 100:617–623
    [Google Scholar]
  19. Pfaller M.A. 1996; Nosocomial candidiasis: emerging species, reservoirs and models of transmisson.. Clin Infect Dis 22: (suppl. 2) 589–594
    [Google Scholar]
  20. Pujol C., Joly S., Lockhart S.R., Noel S., Tibayrenc M., Soll D.R. 1997; Parity among the randomly amplified polymorphic DNA method, multilocus enzyme electrophoresis, and Southern blot hybridization with the moderately repetitive probe Ca3 for fingerprinting Candida albicans. . J Clin Microbiol 35:2348–2358
    [Google Scholar]
  21. Meyer S.A., Ahearn D.G., Yarrow D. 1984; Candida Berkhout.. In The Yeasts pp. 585–884 Edited by Kreger-van Rij N. J. Amsterdam: Elsevier;
    [Google Scholar]
  22. Sadhu C., McEachern M.J., Rustchenko B.E., Schmid J., Soll D.R., Hicks J.B. 1991; Telomeric and dispersed repeat sequences in Candida yeasts and their use in strain identification.. J Bacteriol 173:842–850
    [Google Scholar]
  23. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  24. Scherer S., Stevens D.A. 1987; Application of DNA typing methods to epidemiology and taxonomy of Candida species.. J Clin Microbiol 25:675–679
    [Google Scholar]
  25. Scherer S., Stevens D.A. 1988; A Candida albicans dispersed, repeated gene family and its epidemiologic applications.. Proc Natl Acad Sci USA 851452–1456
    [Google Scholar]
  26. Schmid J., Voss E., Soll D.R. 1990; Computer-assisted methods for assessing strain relatedness in Candida albicans by fingerprinting with the moderately repetitive sequence Ca3.. J Clin Microbiol 28:1236–1243
    [Google Scholar]
  27. Schmid J., Odds F.C., Wiselka M.J., Nicholson K.G., Soll D.R. 1992; Genetic similarity and maintenance of Candida albicans strains from a group of AIDS patients, demonstrated by DNA fingerprinting.. J Clin Microbiol 30:935–941
    [Google Scholar]
  28. Schmid J., Rotman M., Reed B., Pierson C.L., Soll D.R. 1993; Genetic similarity of Candida albicans strains from vaginitis patients and their partners.. J Clin Microbiol 31:39–46
    [Google Scholar]
  29. Schröppel K., Rotman M., Gaiask R., Mac K., Soll D.R. 1994; Evolution and replacement of Candida albicans strains during recurrent vaginitis demonstrated by DNA fingerprinting.. J Clin Microbiol 32:2646–2654
    [Google Scholar]
  30. Sneath P., Sokal R. 1973; Numerical taxonomy.. In The Principles and Practice of Numerical Classification pp. 230–234 San Francisco, CA: W. H. Freeman;
    [Google Scholar]
  31. Sobel J.D. 1996; Candida vulvovaginitis.. Semin Dermatol 15:17–28
    [Google Scholar]
  32. Soll D.R. 1993; DNA fingerprinting of Candida albicans. . J Mycol Med 3:37–44
    [Google Scholar]
  33. Soll D.R. 1996; Computer-assisted analysis of DNA fingerprinting.. In Genes and Genomes in Medically Important Fungi pp. 97–108 Edited by Tanaka K., Yamaguchi Y., Magee P. T. Tokyo: Foundation for Advancement of International Science;
    [Google Scholar]
  34. Soll D.R., Langtimm C.J., McDowell J., Hicks J., Gaiask R. 1987; High-frequency switching in Candida strains isolated from vaginitis patients.. J Clin Microbiol 25:1611–1622
    [Google Scholar]
  35. Soll D.R., Gaiask R., Schmid J., Hanna C., Mac K., Morrow B. 1991; Genetic dissimilarity of commensal strains of Candida spp. carried in different anatomical locations of the same healthy women.. J Clin Microbiol 29:1702–1710
    [Google Scholar]
  36. Spinillo A., Capuzzo E., Egbe T., Baltaro F., Nicola S., Piazzi G. 1995; Torulopsis glabrata vaginitis.. Obstet Gynecol 85:993–998
    [Google Scholar]
  37. Srikantha T., Gutell R.R., Morrow B., Soll D.R. 1994; Partial nucleotide sequence of a single ribosomal RNA coding region and secondary structure of the large subunit 25 s rRNA of Candida albicans. . Curr Genet 26:321–328
    [Google Scholar]
  38. Sullivan D., Bennett D., Henman M., Harwood P., Flint S., Mulcahy F., Shanley D., Coleman D. 1993; Oligonucleotide fingerprinting of isolates of Candida species other than C. albicans and of atypical Candida species from human immunodeficiency virus-positive and AIDS patients.. J Clin Microbiol 31:2124–2133
    [Google Scholar]
  39. Sullivan D., Haynes K., Moran G., Shanley D., Coleman D. 1996; Persistence, replacement, and microevolution of Cryptococcus neoformans strains in recurrent meningitis in AIDS patients.. J Clin Microbiol 34:1739–1744
    [Google Scholar]
  40. Vazquez J.A., Beckley A., Donabedian S., Sobel J.D., Zervos M.J. 1993; Comparison of restriction enzyme analysis versus pulsed-field gradient gel electrophoresis as a typing system for Torulopsis glabrata and Candida species other than C. albicans. . J Clin Microbiol 31:2021–2030
    [Google Scholar]
  41. Voss A., Kluytmans J., Koeleman J., Spanjaard L., Vanden-broucke-Grauls C., Verbrugh H., Vos M., Weersink A., Hoog-kamp-Korstanje J., Meis J. 1996; Occurrence of yeast bloodstream infections between 1987 and 1995 in five Dutch university hospitals.. Eur J Clin Microbiol Infect Dis 15:909–912
    [Google Scholar]
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