1887

Abstract

The viability of cells and the expression of a reporter gene in macrofibres have been examined using cytochemical approaches. Previous studies have shown that macrofibres grow at an exponential rate in fluid environments and undergo complex dynamic motions as they elongate but the behaviour of individual cells in them has never been examined. A fluorescence staining procedure that distinguishes living cells from dead cells was used in the experiments described to examine cells in both left- and right-handed macrofibres. Very few dead cells were found in individual fibres. Their locations appeared to be random, suggesting that neither the extreme shape deformation imposed upon cells in the loop ends of fibres, nor the entrapment of cells in the interior of a fibre compromise viability. In related experiments, initial studies of reporter gene expression during fibre morphogenesis were conducted using a strain previously studied as conventional colonies. Spatial and temporal differences in the appearance of the blue cleavage product of X-Gal were found, suggesting that differential gene expression may arise in macrofibres in contrast to the results found in colonies. Two conclusions may be drawn from the findings: (i) virtually all cells in macrofibres are viable and (ii) all the cells in macrofibres do not always behave in an identical manner.

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1997-12-01
2021-05-17
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References

  1. Belas R., Simon M., Silverman M. 1986; Regulation of lateral flagella gene transcription in Vibrio parabaemoliyticus.. J Bacterial 167:210–218
    [Google Scholar]
  2. Ben-Jacob E., Schochet O., Tenenbaum A., Cohen I., Cszirok A., Vicesek T. 1994; Generic modeling of cooperative growth patterns in bacterial colonies.. Nature 368:46–49
    [Google Scholar]
  3. Kroos L., Kaiser D. 1987; Expression of many developmentally regulated genes in Myxococcus depends on a sequence of cell interactions.. Genes Dev 1:840–854
    [Google Scholar]
  4. Lawrence P.A. 1992 The Making of a Fly. London: Blackwell;
    [Google Scholar]
  5. Mendelson N.H. 1976; Helical growth of Bacillus subtilis: a new model of cell growth.. Proc Natl Acad Sci USA 73:1740–1744
    [Google Scholar]
  6. Mendelson N.H. 1978; Helical Bacillus subtilis macrofibers: morphogenesis of a bacterial multicellular macroorganism.. Proc Natl Acad Sci USA 75:2478–2482
    [Google Scholar]
  7. Mendelson N.H. 1982; Dynamics of Bacillus subtilis helical macrofiber morphogenesis: writhing, folding, close packing and contraction.. J Bacteriol 151:438–449
    [Google Scholar]
  8. Mendelson N.H. 1992; Self-assembly of bacterial macrofibers: a system based upon hierarchies of helices.. Mat Res Soc Sytnp Proc 255:43–54
    [Google Scholar]
  9. Mendelson N.H., Favre D. 1987; Regulation of Bacillus subtilis macrofiber twist development by ions: effects of mag¬nesium and ammonium.. J Bacteriol 169:519–525
    [Google Scholar]
  10. Mendelson N.H., Salhi B. 1996; Patterns of reporter gene expression in the phase diagram of Bacillus subtilis colony forms.. J Bacteriol 178:1980–1989
    [Google Scholar]
  11. Mendelson N.H., Thwaites J.J. 1989; Do forces and the physical nature of cellular materials govern biological processes ? . Comment Theor Biol 1:217–235
    [Google Scholar]
  12. Mendelson N.H., Salhi B. 1997; Physical and genetic consequences of multicellularity in Bacillus subtilis. . In Bacteria as Multicellular Organisms pp. 339–365 Shapiro J.A., Dworkin M. Edited by New York: Oxford University Press;
    [Google Scholar]
  13. Roberts J.L. 1938; Evidence of a rotational growth factor in Bacillus mycoides.. Science 87:260–261
    [Google Scholar]
  14. Roszak D.B., Colwell R.R. 1987; Survival strategies of bacteria in natural environments.. Microbiol Rev 51:365–379
    [Google Scholar]
  15. Salhi B., Mendelson N.H. 1993; Patterns of gene expression in Bacillus subtilis colonies.. J Bacteriol 175:5000–5008
    [Google Scholar]
  16. Shapiro J.A. 1997; Multicellularity: the rule, not the exception. Lessons from Escherichia coli colonies.. In Bacteria as Multicellular Organisms pp. 14–49 Shapiro J.A., Dworkin M. Edited by New York: Oxford University Press;
    [Google Scholar]
  17. Thwaites J.J., Mendelson N.H. 1985; Biomechanics of bacterial walls: studies of bacterial thread made from Bacillus subtilis.. Proc Natl Acad Sci USA 82:2163–2167
    [Google Scholar]
  18. Yarmolinsky M.B. 1995; Programmed cell death in bacterial populations.. Science 267:836–837
    [Google Scholar]
  19. Youngman P. 1987; Plasmid vectors for recovering and exploit¬ ing Tn917 transpositions in Bacillus and other Gram-positive bacteria.. In Plasmids, A Practical Approach pp. 79–103 Hardy K.G. Edited by Oxford: IRL Press;
    [Google Scholar]
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