RT Journal Article SR Electronic(1) A1 Ives, Paul R. A1 Bushell, Michael E.YR 1997 T1 Manipulation of the physiology of clavulanic acid production in Streptomyces clavuligerus JF Microbiology, VO 143 IS 11 SP 3573 OP 3579 DO https://doi.org/10.1099/00221287-143-11-3573 PB Microbiology Society, SN 1465-2080, AB This paper reports a novel use of cluster analysis for the identification of intermediary metabolites that are produced at rates closely correlated with those of antibiotic biosynthesis. This information was used to devise culture feeds resulting in enhanced production of clavulanic acid, an antibiotic of current worldwide commercial interest. The feeding strategies apparently alleviated a rate-limiting supply of the C3 precursor of clavulanic acid. C3 limitation may be a consequence of unusual nitrogen and carbon metabolism in Streptomyces clavuligerus. This approach has potential as a generic method for influencing biosynthetic pathway fluxes using feeds without knowledge of the biosynthetic pathway., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-143-11-3573