1887

Abstract

Two neighbouring genes, and at 59 min on the chromosome are both required for stationary-phase survival. Operon fusions of the putative promoter regions in front of (P2) or (P3) with the reporter gene were constructed to study the transcriptional regulation of and Both promoter regions were able to activate -galactosidase activity in a growth-phase-dependent way in either rich or minimal medium. Induction from both promoters reached the highest level in late stationary phase and was independent of the gene. Spent medium from early as well as late stationary-phase cultures could induce the expression of either promoter even after dialysis or boiling. A high cell density could induce the promoters more rapidly but not to a greater extent. It is proposed that the induction might be correlated with the decline in growth rate of the cells. The induction patterns of either P2 or P3 were very similar, can thus be transcribed from both the P2 and P3 promoters that are regulated in similar ways.

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1997-11-01
2021-05-14
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References

  1. Bremer H., Dennis P.P. 1987; Modulation of chemical composition and other parameters of the cell by growth rate.. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology pp. 1527–1542 Neidhardt F.C., Ingraham J.L., Brooks Low K., Magasanik B., Schaechter M., Umbarger H.E. Edited by Washington, DC:: American Society for Microbiology.;
    [Google Scholar]
  2. Brennan T.V., Anderson J.W., Jia Z., Waygood E.B., Clarke S. 1994; Repair of spontaneously deamidated HPr phospho-carrier protein catalyzed by the L-isoaspartate-(D-aspartate) O-methyltransferase.. J Biol Chem 269:24586–24595
    [Google Scholar]
  3. Bult C.J., White O., Olsen G.J. 37 other authors 1996; Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.. Science 273:1058–1073
    [Google Scholar]
  4. Clarke S. 1985; Protein carboxyl methyltransferases: Two distinct classes.. Annu Rev Biochem 54:479–506
    [Google Scholar]
  5. Fleischmann R.D., Adams M.D., White O. 37 other authors 1995; Whole-genome random sequencing and assembly of Haemophilus influenzae Rd.. Science 269:496–512
    [Google Scholar]
  6. Fraser C.M., Gocayne J.D., White O. 26 other authors 1995; The minimal gene complement of Mycoplasma genitalium.. Science 270:397–403
    [Google Scholar]
  7. Fu J., Ding L., Clarke S. 1991; Purification, gene cloning, and sequence analysis of an L-isoaspartyl protein carboxyl methyl-transferase from Escherichia coli.. J Biol Chem 266:14562–14572
    [Google Scholar]
  8. Gordia S., Gutierrez C. 1996; Growth-phase-dependent expression of the osmotically inducible gene osmC of Escherichia coli K-12.. Mol Microbiol 19:729–736
    [Google Scholar]
  9. Harding J., Beswick H.T., Ajiboye R., Huby R., Blakytny R., Rixon K.C. 1989; Nonenzymatic post-translational modification of proteins in aging. A review.. Mech Ageing Dev 50:7–16
    [Google Scholar]
  10. Hengge-Aronis R. 1993; Survival of hunger and stress: The role of rpoS in early stationary phase gene regulation in E. coli.. Cell 72:165–168
    [Google Scholar]
  11. Hengge-Aronis R., Fischer V. 1992; Identification and molecular analysis of glgS, a novel growth-phase-regulated and rpoS-dependent gene involved in glycogen synthesis in Escherichia coli.. Mol Microbiol 6:1877–1886
    [Google Scholar]
  12. Ichikawa J.K., Li C., Fu J., Clarke S. 1994; A gene at 59 minutes on the Escherichia coli chromosome encodes a lipo-protein with unusual amino acid repeat sequences.. J Bacteriol 176:1630–1638
    [Google Scholar]
  13. Ingrosso D., Fowler A.V., Bleibaum J., Clarke S. 1989; Sequence of the D-aspartyl/L-isoaspartyl protein methyl-transferase from human erythrocytes.. J Biol Chem 264:20131–20139
    [Google Scholar]
  14. Johnson B.A., Murray E.D. Jr Clarke S., Glass D.B., Aswad D.W. 1987; Protein carboxyl methyltransferase facilitates conversion of atypical L-isoaspartyl peptides to normal L-aspartyl peptides.. J Biol Chem 262:5622–5629
    [Google Scholar]
  15. Johnson B.A., Ngo S.Q., Aswad D.W. 1991; Widespread phylogenetic distribution of a protein methyltransferase that modifies L-isoaspartyl residues.. Biochem Int 24:481–487
    [Google Scholar]
  16. Kagan R.M., Clarke S. 1995; Protein L-isoaspartyl methyl-transferase from the nematode Caenorhabditis elegans: genomic structure and substrate specificity.. Biochemistry 34:10794–10806
    [Google Scholar]
  17. Kolter R., Siegele D.A., Tormo A. 1993; The stationary phase of the bacterial life cycle.. Annu Rev Microbiol 47:855–874
    [Google Scholar]
  18. Lange R., Hengge-Aronis R. 1991; Identification of a central regulator of stationary-phase gene expression in Escherichia coli.. Mol Microbiol 5:49–59
    [Google Scholar]
  19. Li C. 1993 The physiological functions of the L-isoaspartyl protein methyltransferase and related proteins in bacteria. PhD thesis Department of Chemistry and Biochemistry, University of California, Los Angeles, CA, USA.:
    [Google Scholar]
  20. Li C., Clarke S. 1992a; Distribution of an L-isoaspartyl protein methyltransferase in eubacteria.. J Bacteriol 174:355–361
    [Google Scholar]
  21. Li C., Clarke S. 1992b; A protein methyltransferase specific for altered aspartyl residues is important in Escherichia colistationary-phase survival and heat-shock resistance.. Proc Natl Acad Sci USA 899885–9889
    [Google Scholar]
  22. Li C., Ichikawa J.K., Ravetto J.J., Kuo H.-C., Fu J.C., Clarke S. 1994; A new gene involved in stationary-phase survival located at 59 minutes on the Escherichia coli chromosome.. J Bacteriol 176:6015–6022
    [Google Scholar]
  23. Loewen P.C., Hengge-Aronis R. 1994; The role of the sigma factor σS (KatF) in bacterial global regulation.. Annu Rev Microbiol 48:53–80
    [Google Scholar]
  24. Matin A., Auger E.A., Blum P.H., Schultz J.E. 1989; Genetic basis of starvation survival in nondifferentiating bacteria.. Annu Rev Microbiol 43:293–316
    [Google Scholar]
  25. McFadden P.N., Clarke S. 1982; Methylation at D-aspartyl residues in erythrocytes: possible step in the repair of aged membrane proteins.. Proc Natl Acad Sci USA 792460–2464
    [Google Scholar]
  26. McFadden P.N., Clarke S. 1987; Conversion of isoaspartyl peptides to normal peptides: implications for the cellular repair of damaged proteins.. Proc Natl Acad Sci USA 842595–2599
    [Google Scholar]
  27. Miller J.H. 1992 A Short Course in Bacterial Genetics: a Laboratory Manual and Handbook for Escherichia coli and Related Bacteria. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  28. Mudgett M.B., Clarke S. 1993; Characterization of plant L-isoaspartyl methyltransferases that may be involved in seed survival: Purification, cloning, and sequence analysis of the wheat germ enzyme.. Biochemistry 32:11100–11111
    [Google Scholar]
  29. Mulvey M.R., Switala J., Borys A., Loewen P. 1990; Regulation of transcription of katE and katF in Escherichia coli.. J Bacteriol 172:6713–6720
    [Google Scholar]
  30. O̓Connor C.M., Clarke S. 1985; Specific recognition of altered polypeptides by widely distributed methyltransferases.. Biochem Biophys Res Commun 132:1144–1150
    [Google Scholar]
  31. Roszak D.B., Colwell R.R. 1987; Survival strategies of bacteria in the natural environment.. Microbiol Rev 51:365–379
    [Google Scholar]
  32. Siegele D.A., Kolter R. 1992; Life after log.. J Bacteriol 174:345–348
    [Google Scholar]
  33. Simons R.W., Houman F., Kleckner N. 1987; Improved single and multicopy lac-based cloning vectors for protein and operon fusions.. Gene 53:85–96
    [Google Scholar]
  34. Stadtman E.R. 1988; Protein modification in aging.. J Gerontol 43:B112–B120
    [Google Scholar]
  35. Swanson R.V., Sanna M.G., Simon M.I. 1996; Thermostable chemotaxis proteins from the hyperthermophilic bacterium Thermotoga maritima.. J Bacteriol 178:484–489
    [Google Scholar]
  36. Tanaka K., Takahashi H. 1994; Cloning, analysis and expression of an rpoS homologue gene from Pseudomonas aeruginosa PAO1.. Gene 150:81–85
    [Google Scholar]
  37. Theisen M., Rioux C.R., Potter A.A. 1993; Molecular cloning, nucleotide sequence, and characterization of lppB, encoding an antigenic 40-kilodalton lipoprotein of Haemophilus somnus.. Infect Immun 61:1793–1798
    [Google Scholar]
  38. Visick J.E., Clarke S. 1995; Repair, refold, recycle: how bacteria can deal with spontaneous and environmental damage to proteins.. Mol Microbiol 16:835–845
    [Google Scholar]
  39. Weichart D., Lange R., Henneberg N., Hengge-Aronis R. 1993; Identification and characterization of stationary phase- inducible genes in Escherichia coli.. Mol Microbiol 10:407–420
    [Google Scholar]
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