1887

Abstract

spp. are well recognized as primary pathogens in animals and in people. To isolate and define the genetic regions encoding major surface antigens of genomic DNA of the type strain of the species, RMIT-32A, was cloned into a cosmid vector, pLA2917, in and the resulting genomic library was screened using antiserum raised to the parent strain. Six cosmid clones were found to express a series of immunoreactive bands in the 15-25 kDa range. These bands were proteinase K-resistant and were found in the LPS fraction of the cells, suggesting that the recombinant cosmids expressed lipo-oligosaccharide (LOS) antigen(). The minimum DNA insert size required for expression of LOS antigen() in was 11-8 kb. This region was subcloned into the plasmid vector pBR322. The partial sequencing of the 11.8 kb region showed that it contains two ORFs, designated and showing homology with the gene from and the gene from Both genes are involved in LPS synthesis. The region also contained a sequence homologous to the gene of and which is involved in core oligosaccharide synthesis.

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1997-11-01
2021-04-22
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