RT Journal Article SR Electronic(1) A1 Lapidus, Alia A1 Galleron, Nathalie A1 Sorokin, Alexei A1 Ehrlich, S. DuskoYR 1997 T1 Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region JF Microbiology, VO 143 IS 11 SP 3431 OP 3441 DO https://doi.org/10.1099/00221287-143-11-3431 PB Microbiology Society, SN 1465-2080, AB The 200 kb region of the Bacillus subtilis chromosome spanning from 255 to 275° on the genetic map was sequenced. The strategy applied, based on use of yeast artificial chromosomes and multiplex Long Accurate PCR, proved to be very efficient for sequencing a large bacterial chromosome area. A total of 193 genes of this part of the chromosome was classified by level of knowledge and biological category of their functions. Five levels of gene function understanding are defined. These are: (i) experimental evidence is available of gene product or biological function; (ii) strong homology exists for the putative gene product with proteins from other organisms; (Hi) some indication of the function can be derived from homologies with known proteins; (iv) the gene product can be clustered with hypothetical proteins; (v) no indication on the gene function exists. The percentage of detected genes in each category was: 20, 28, 20, 15 and 17, respectively. In the sequenced region, a high percentage of genes are implicated in transport and metabolic linking of glycolysis and the citric acid cycle. A functional connection of several genes from this region and the genes close to 140° in the chromosome was also observed., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-143-11-3431