RT Journal Article SR Electronic(1) A1 Seoane, Asunción A1 Navas, Jesús A1 García Lobo, Juan M.YR 1997 T1 Targets for pSAM2 integrase-mediated site-specific integration in the Mycobacterium smegmatis chromosome JF Microbiology, VO 143 IS 10 SP 3375 OP 3380 DO https://doi.org/10.1099/00221287-143-10-3375 PB Microbiology Society, SN 1465-2080, AB An improved integrative cassette from plasmid pSAM2 has been constructed containing plasmid int and attP genes but excluding the xis gene, which should result in increased stability by suppression of the excision reaction. This cassette was included in both suicide and thermosensitive plasmids and used for integration in Mycobacterium smegmatis. Suicide plasmids containing this cassette integrated at a single site (attB1) in the M. smegmatis chromosome. The sequence of the attB1 site has been determined and was identified as a putative tRNAPro gene. Thermosensitive plasmids containing the cassette integrated both at the same attB1 site and at other different sites, often giving rise to simultaneous integration at two sites. A second integration site (attB2) has been sequenced, which was located in the region encoding 16S rRNA of one of the two rrn operons of M. smegmatis. , UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-143-10-3375