An improved integrative cassette from plasmid pSAM2 has been constructed containing plasmid and genes but excluding the gene, which should result in increased stability by suppression of the excision reaction. This cassette was included in both suicide and thermosensitive plasmids and used for integration in Suicide plasmids containing this cassette integrated at a single site () in the chromosome. The sequence of the site has been determined and was identified as a putative tRNA gene. Thermosensitive plasmids containing the cassette integrated both at the same site and at other different sites, often giving rise to simultaneous integration at two sites. A second integration site () has been sequenced, which was located in the region encoding 16S rRNA of one of the operons of


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