1887

Abstract

A cell culture system, employing the fish cell line (EPC), was developed to study the synthesis of intracellular antigen and the expression of putative virulence factors by EPC cultures infected with could be maintained for 7 weeks, during which the pathogen multiplied intracellularly. Immunohistochemical examination of infected cultures revealed the production of the p57 antigen, haemolysin and cytolysin. The intracellular nature of the infection was confirmed by transmission electron microscopic examination of EPC monolayers. A comparison of the relative virulence of bacterial cells cultured in EPC cells and on agar plates revealed that the former were markedly more virulent in challenge experiments with juvenile rainbow trout ( Walbaum). The EPC cell culture model provided a system for the study of under more natural conditions than those achieved with plate culture techniques.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-143-10-3349
1997-10-01
2021-04-15
Loading full text...

Full text loading...

/deliver/fulltext/micro/143/10/mic-143-10-3349.html?itemId=/content/journal/micro/10.1099/00221287-143-10-3349&mimeType=html&fmt=ahah

References

  1. Alpuche-Aranda C.M., Racoosin E.L., Swanson I.A., Miller S.I. 1994; Salmonella. J Exp Med 179:601–608
    [Google Scholar]
  2. Austin B., Austin D.A. 1993 Bacterial Fish Pathogens: Disease in Farmed and Wild Fish, 2nd edn. Chichester: Ellis Horwood;
    [Google Scholar]
  3. Austin B., Embley T.M., Goodfellow M. 1983; Selective isolation of renibacterium salmoninarum. FEMS Microbiol Lett 17:111–114
    [Google Scholar]
  4. Bandfn I., Santos Y., Barja J.L., Toranzo A.E. 1989; Influence of the growth conditions on the hydrophobicity of renibacterium salmoninarum evaluated by different methods. FEMS Microbiol Lett 60:71–78
    [Google Scholar]
  5. Bandfn I., Santos Y., Bruno D.W., Raynard R.S., Toranzo A.E., Barja J.L. 1991; Lack of biological activities in the extracellular products of renibacterium salmoninarum. Can J Fish Aquat Sci 48:421–425
    [Google Scholar]
  6. Bell G.R., Traxler G.S., Dworschak C. 1988; Development in vitro and pathogenicity of an erythromycin-resistant strain of renibacterium salmoninarum, the causative agent of bacterial kidney disease in salmonids. Dis Aquat Org 4:19–25
    [Google Scholar]
  7. Bruno D.W., Munro A.L.S. 1986; Uniformity in the biochemical properties of renibacterium salmoninarum isolates obtained from several sources. FEMS Microbiol Lett 33:247–250
    [Google Scholar]
  8. Daly J.G., Stevenson R.M.W. 1987; Hydrophobic and haemagglutinating properties of renibacterium salmoninarum. J Gen Microbiol 133:3575–3580
    [Google Scholar]
  9. Daly I.G., Stevenson R.M.W. 1989; Agglutination of salmonid spermatozoa by. J Aquat Anim Health 1:163–164
    [Google Scholar]
  10. Daly J.G., Stevenson R.M.W. 1990; Characterization of the renibacterium salmoninarum haemagglutinin. J Gen Microbiol 136:949–953
    [Google Scholar]
  11. Evelyn T.P.T. 1993; Bacterial kidney disease-bkd. In Bacterial Diseases of Fish Inglis V., Roberts R. J., Bromage N. R. Oxford: Blackwell;
    [Google Scholar]
  12. Evelyn T.P.T., Bell G.R., Prosperi-Porta L., Ketcheson J.E. 1989; A simple technique for accelerating the growth of the kidney disease bacterium renibacterium salmoninarum on a commonly used culture medium (Kdm2). Dis Aquat Org 7:231–234
    [Google Scholar]
  13. Evenden A.J., Gilpin M.L., Munn C.B. 1990; The cloning and expression of a gene encoding haemolytic activity from the fish pathogen renibacterium salmoninarum. FEMS Microbiol Lett 71:31–34
    [Google Scholar]
  14. Evenden A.J., Grayson T.H., Gilpin M.L., Munn C.B. 1993; ). Annu Rev Fish Dis 3:87–104
    [Google Scholar]
  15. Flañio E., López-Fierro M.P., Razquin B.E., Villena A. 1996; Renibacterium salmoninarum. Fish Shellfish Immunol 6:173–184
    [Google Scholar]
  16. Flañio E., López-Fierro P., Razquin B., Kaattari S.L., Villena A. 1996; Histopathology of the renal and splenic haemopoietic tissues of coho salmon oncorhynchus kisutch experimentally infected with renibacterium salmoninarum. Dis Aquat Org 24:107–115
    [Google Scholar]
  17. Flañio E., Kaattari S.L., Razquin B., Villena A. 1996; Histopathology of the thymus of coho salmon oncorhynchus kisutch experimentally infected with renibacterium salmoninarum. Dis Aquat Org 26:11–18
    [Google Scholar]
  18. Fryer J.L., Sanders J.E. 1981; Bacterial kidney disease of salmonid fish. Annu Rev Microbiol 35:273–298
    [Google Scholar]
  19. Getchell R.G., Rohovec J.S., Fryer J.L. 1985; Comparison of renibacterium salmoninarum isolates by antigen analysis. Fish Pathol 20:149–159
    [Google Scholar]
  20. Grayson T.H. 1993 Molecular Cloning and Characterisation of Potential Vaccine Antigens from Renibacterium Salmoninarum UK: University of Plymouth;
    [Google Scholar]
  21. Grayson T.H., Evenden A.J., Gilpin M.L., Munn C.B. 1995; A gene from renibacterium salmoninarum encoding a product which shows homology to bacterial zinc-metalloproteases. Microbiology 141:1331–1341
    [Google Scholar]
  22. Grayson T.H., Evenden A.J., Gilpin M.L., Munn C.B. 1995; Production of. Dis Aquat Org 22:153–156
    [Google Scholar]
  23. Griffiths S.G., Olivier G., Fildes J., Lynch W.H. 1991; Comparison of western blot, direct fluorescent antibody and drop plate culture methods for the detection of renibacterium salmoninarum in atlantic salmon (Salmo salar l.). Aquaculture 97:117–129
    [Google Scholar]
  24. Gutenberger S.K. 1993 Phylogeny and Intracellular Survival of Renibacterium Salmoninarum USA: Oregon State University;
    [Google Scholar]
  25. Kaattari S., Getchell R., Turaga P., Irwin M., Wiens G. 1986 Development of a Vaccine for Bacterial Kidney Disease in Salmon. Annual Report 1985 Bonneville Power Administration, Division of Fish and Wildlife;
    [Google Scholar]
  26. McIntosh D., Austin B. 1996; The validity of western blotting for the diagnosis of bacterial kidney disease based on the detection of the p57 antigen of renibacterium salmoninarum. J Microbiol Methods 25:329–335
    [Google Scholar]
  27. Maul6n N.P., Morales P.J., Aruti D., Figueroa J.E., Concha M.I., Krauskopf M., Le6n G. 1996; Identification of a renibacterium salmoninarum dna fragment associated with bacterial internalization into chse-cultured cells. FEMS Microbiol Lett 135:37–43
    [Google Scholar]
  28. Rose A.S., Levine R.P. 1992; Complement mediated opsonisation and phagocytosis of renibacterium salmoninarum. Fish Shellfish Immunol 2:223–240
    [Google Scholar]
  29. Sakai M., Ogasawara F., Atsuta S., Kobayashi M. 1989; Comparative sensitivity of carp cyprinus carpio l and rainbow trout salmo gairdneri richardson, to. J Fish Dis 12:367–372
    [Google Scholar]
  30. Salyers A.A., Whitt D.D. 1994 Bacterial Pathogenesis: A Molecular Approach Washington, DC: American Society for Microbiology;
    [Google Scholar]
  31. Turaga P., Wiens G., Kaattari S. 1987; Bacterial kidney disease: The potential role of soluble protein antigen(s). J Fish Biol Suppl A 31:191–194
    [Google Scholar]
  32. Wiens G.D., Kaattari S. 1989; Monoclonal antibody analysis of common surface protein(S) of renibacterium salmoninarum. Fish Pathol 24:1–7
    [Google Scholar]
  33. Wiens G.D., Kaattari S.L. 1991; Monoclonal antibody characterization of a leuko-agglutinin produced by renibacterium salmoninarum. Infect Immun 59:631–637
    [Google Scholar]
  34. Wolke R.E. 1975; Pathology of bacterial and fungal disease affecting fish. In The Pathology of Fishes pp. 76–78 Edited by Ribelin W. E., Migaki G. Madison: University of Wisconsin Press;
    [Google Scholar]
  35. Young C.L., Chapman G.B. 1978; Ultrastructural aspects of the causative agent and renal histopathology of bacterial kidney disease in brook trout (Salvelinus fontinalis). J Fish Res Board Can 35:1234–1248
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-143-10-3349
Loading
/content/journal/micro/10.1099/00221287-143-10-3349
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error