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Volume 143, Issue 10

is unable to produce pyrroloquinoline quinone (PQQ) Free

Abstract

Many bacteria can synthesize the cofactor pyrroloquinoline quinone (PQQ), a cofactor of several dehydrogenases, including glucose dehydrogenase (GCD). Among the enteric bacteria, has been shown to contain the genes required for PQQ biosynthesis. and were thought to be unable to synthesize PQQ but it has been reported that strain EF260, a derivative of FB8, can synthesize PQQ after mutation and can oxidize glucose to gluconate via the GCD/PQQ pathway (F. Biville, E. Turlin & F. Gasser, 1991, 137, 1775-1782). We have reinvestigated this claim and conclude that it is most likely erroneous. (i) Strain EF260, isolated originally by Biville and coworkers, was unable to synthesize a holo-enzyme GCD unless PQQ was supplied to the growth medium. No GCD activity could be detected in membrane fractions. (ii) The amount of PQQ detected in the growth medium of EF260 was very low and not very different from that found in a medium with its parent strain or in a medium containing no cells. (iii) EF260 cells were unable to produce gluconate from glucose via the PQQ/GCD pathway. (iv) Introduction of a ::Cm deletion in EF260, eliminating GCD, did not affect glucose metabolism. This suggested a pathway for glucose metabolism other than the PQQ/GCD pathway, (v) Glucose uptake and metabolism in EF260 involved a low-affinity transport system of unknown identity, followed most likely by phosphorylation via glucokinase. It is concluded that cannot synthesize PQQ and that it lacks genes required for PQQ biosynthesis.

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  • Accepted:
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  • Published Online:
Keyword(s): Escherichia coli , glucose dehydrogenase , glucose metabolism , PQQ and quinoprotein
© Society for General Microbiology 1997
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1997-10-01
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Escherichia coli is unable to produce pyrroloquinoline quinone (PQQ)
Microbiology 143, 3149 (1997); https://doi.org/10.1099/00221287-143-10-3149
/content/journal/micro/10.1099/00221287-143-10-3149
/content/journal/micro/10.1099/00221287-143-10-3149
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