@article{mbs:/content/journal/micro/10.1099/00221287-143-1-73, author = "Hirvas, Laura and Nurminen, Marjatta and Helander, Ilkka M. and Vuorio, Riitta and Vaara, Martti", title = "The Lipid a Biosynthesis Deficiency of the Escherichia Coli Antibiotic-Supersensitive Mutant LH530 is Suppressed by a Novel Locus, ORF195", journal= "Microbiology", year = "1997", volume = "143", number = "1", pages = "73-81", doi = "https://doi.org/10.1099/00221287-143-1-73", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-143-1-73", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "lipid A", keywords = "permeability mutant", keywords = "Escherichia coli", keywords = "ORF195", keywords = "antibiotic-supersensitive mutant", abstract = "A new mutant of Escherichia coli K-12 supersensitive to both hydrophobic and large hydrophilic antibiotics was isolated and characterized. The mutant grew well at 28 °C, poorly at 37 °C, and did not grow at 42 °C. The rate of its lipid A biosynthesis was reduced as compared to that of the parent strain. This deficiency was rescued by a novel locus, ORF195, the function of which has not been elucidated. ORF195 is located in the 76 min region in the E. coli chromosome and encodes a hypothetical 21.8 kDa protein with no signal sequence. ORF195 isolated from the mutant strain had an identical sequence to the wild-type allele, indicating a suppressor function of the gene product.", }