A new mutant of K-12 supersensitive to both hydrophobic and large hydrophilic antibiotics was isolated and characterized. The mutant grew well at 28 °C, poorly at 37 °C, and did not grow at 42 °C. The rate of its lipid A biosynthesis was reduced as compared to that of the parent strain. This deficiency was rescued by a novel locus, ORF195, the function of which has not been elucidated. ORF195 is located in the 76 min region in the chromosome and encodes a hypothetical 21.8 kDa protein with no signal sequence. ORF195 isolated from the mutant strain had an identical sequence to the wild-type allele, indicating a suppressor function of the gene product.


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