Cultures of Vibrio cholerae O1, biotype El Tor, from the current epidemic of cholera in the Western Hemisphere, and of the new V. cholerae serogroup O139, from the current outbreak in India and Bangladesh, revealed marked colonial heterogeneity when received by the authors. By comparison with reference colony types, using a stereoscope and transmitted oblique illumination, colonies of approximately 10 different degrees of opacity could be distinguished. In contrast, strains freshly isolated from patients and rapidly and carefully preserved were more homogeneous although still differentiable by this technique. These (and older) observations prompted the questions: (1) why is a V. cholerae colony opaque or translucent? and (2) what benefit is it to the vibrios to vary their colonial appearance? The observed changes in colonial opacity, which are reversible, are sometimes (rarely) accompanied by changes in virulence for infant rabbits and, more frequently, by other phenotypic variations including the ability to produce poly-β-hydroxybutyrate inclusion bodies on glycerol-containing medium, the degree of encapsulation in O139, changes in outer-membrane proteins, alteration in lipopolysaccharide structure, changes in expression of glycolytic pathways, and differences in ability to survive under adverse conditions. Colonial variations in choleragenic vibrios are phenotypically multifactorial. The genetic mechanisms(s) underlying the observed phenotypic changes remain to be defined.
BlakeP.A.1994; Endemic cholera in Australia and the United States.. In Vibrio cholerae and Cholera: Molecular to Global Perspectives pp. 309–319 Edited by
WachsmuthI.K.,
BlakeP.A.,
OlsvikO.
Washington, DC: American Society for Microbiology;
ColwellR.R.,
HuqA.1994; Vibrios in the environment: viable but nonculturable Vibrio cholerae.. In Vibrio cholerae and Cholera: Molecular to Global Perspectives pp. 117–133 Edited by WachsmuthI.K.,
BlakeP.A.,
OlsvikO.
Washington, DC: American Society for Microbiology;
ColwellR.R.,
SpiraW.M.1992; The ecology of Vibrio cholerae.. In Cholera pp. 107–127 Edited by
BaruaD.,
GreenoughW.B.III
New York: Plenum Medical Book Co;
FilipC.,
FletcherG.,
WulffJ.L.,
EarhartC.F.1973; Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate.. J Bacteriol 115:717–722
FinkelsteinR.A.1962; Vibriocidal antibody inhibition (VAI) analysis: a technique for the identification of the predominant vibriocidal antibodies in serum and for the recognition and identification of Vibrio cholerae antigens.. J Immunol 89:264–271
HolmesK.K.,
VasilM.L.,
FinkelsteinR.A.1975; Studies on toxinogenesis in Vibrio cholerae. III. Characterization of non-toxinogenic mutants in vitro and in experimental animals.. J Clin Invest 55:551–560
HoodM.A.,
GuckertJ.B.,
WhiteD.C.,
DeckF.1986; Effect of nutrient deprivation on lipid, carbohydrate, DNA, RNA, and protein levels in Vibrio cholerae.. Appl Environ Microbiol 52:788–793
JohnsonJ.A.,
SallesC.A.,
PanigrahiP.,
AlbertM.J.,
WrightA.C.,
JohnsonR.J.,
MorrisJ.G.Jr1994; Vibrio cholerae 0139 synonym Bengal is closely related to Vibrio cholerae El Tor but has important differences.. Infect Immun 62:2108–2110
KaysnerC.A.,
HillW.E.1994; Toxigenic Vibrio cholerae Ol in food and water.. In Vibrio cholerae and Cholera: Molecular to Global Perspectives pp. 27–39 Edited by
WachsmuthI.K.,
BlakeP.A.,
OlsvikO.
Washington, DC: American Society for Microbiology;
KimuraA.,
HansenE.J.1986; Antigenic and phenotypic variations of Haemophilus influenzae type b lipopolysaccharide and their relationship to virulence.. Infect Immun 51:69–79
MarkwellM.A.K.,
HaasS.M.,
BieberL.L.,
TolbertN.E.1978; A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples.. Anal Biochem 87:206–210
MillsJ.A.,
VenkatesanM.M.,
BaronL.S.,
BuysseJ.M.1992; Spontaneous insertion of an ISl-like element into the virF gene is responsible for avirulence in opaque colonial variants of Shigella flexneri 2a.. Infect Immun 60:175–182
MintzE.D.,
PopovicT.,
BlakeP.A.1994; Transmission of Vibrio cholerae.. In Vibrio cholerae and Cholera: Molecular to Global Perspectives pp. 345–356 Edited by WachsmuthI.K.,
BlakeP.A.,
OlsvikO.
Washington, DC: American Society for Microbiology;
ÖstlingJ.,
HolmquistL.,
FlärdhK.,
SvenbladB.,
Jouper-JaanÅ.,
KjellebergS.1993; Starvation and Recovery of Vibrio.. In Starvation in Bacteria pp. 103–127 Edited by
KjellebergS.
New York & London: Plenum Press;
PageW.J.,
ManchakJ.,
RudyB.1992; Formation of poly(hydroxybutyrate-co-hydroxyvalerate) by Azotobacter vinelandii UWD.. Appl Environ Microbiol 58:2866–2873
PageW.J.,
KnospO.1989; Hyperproduction of poly-β- hydroxybutyrate during exponential growth of Azotobacter vinelandii UWD.. Appl Environ Microbiol 55:1334–1339
RavelJ.,
KnightI.T.,
MonahanC.E.,
HillR.T.,
ColwellR.R.1995; Temperature-induced recovery of Vibrio cholerae from the viable but non-culturable state: growth or resuscitation?. Microbiology 141:377–383
SenguptaD.K.,
Boesman-FinkelsteinM.,
FinkelsteinR.A.1996; Antibody against the capsule of Vibrio cholerae 0139 protects against experimental challenge.. Infect Immun 64:343–345
SunW.,
CaoJ.-G.,
TengK.,
MeighenE.A.1994; Biosynthesis of poly-β-hydroxybutyrate in the luminescent bacterium, Vibrio harveyi, and regulation by the lux autoinducer, N-(3-hydroxybutanoyl)homoserine lactone.. J Biol Chem 269:20785–20790
TauxeR.,
SeminarioL.,
TapiaR.,
LibelM.1994; The Latin American epidemic.. In Vibrio cholerae and Cholera: Molecular to Global Perspectives pp. 321–344 Edited by
WachsmuthI.K.,
BlakeP.A.,
OlsvikO.
Washington, DC: American Society for Microbiology;
WeiserJ.N.1993; Relationship between colony morphology and the life cycle of Haemophilus influenzae: the contribution of lipopolysaccharide phase variation to pathogenesis.. J Infect Dis 168:672–680
WeiserJ.N.,
ChongS.T.H.,
GreenbeyD.,
FongW.1995; Identification and characterization of a cell envelope protein of Haemophilus influenzae contributing to phase variation in colony opacity and nasopharyngeal colonization.. Mol Microbiol 17:555–564
WiseK.S.,
YogevD.,
RosengartenR.1992; Antigenic variation.. In Mycoplasmas: Molecular Biology and Pathogenesis pp. 473–490 Edited by
ManiloffJ.,
McElhaneyR.N.,
FinchL.R.,
BasemanJ.B.
Washington, DC: American Society for Microbiology;