bofC Encodes a Putative Forespore Regulator of the Bacillus Subtilis σk Checkpoint Free

Abstract

A mutation, , that restores σ activation in strains unable to produce active σ has been identified. This mutation defines a new sporulation gene, , that has been cloned and sequenced and encodes a 19 kDa protein. is transcribed in the forespore by RNA polymerase associated with the transcription factors σ (Eσ) and σ (Eσ). BofC acts negatively on SpolVB and the results described suggest that BofC regulates SpolVB activity and its intercompartmental signalling role in the σ checkpoint

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1997-01-01
2024-03-29
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References

  1. Cheo D.L., Bayles K.W., Yasbin R.E. 1991; Cloning and characterization of DNA damage-inducible promoter regions from Bacillus subtilis.. J Bacteriol 173:1696–1703
    [Google Scholar]
  2. Cutting S.M., Vander-Horn P.B. 1990; Genetic analysis.. In Molecular Biological Methods for Bacillus pp. 27–74 Harwood C.R., Cutting S.M. Edited by Chichester:: John Wiley.;
    [Google Scholar]
  3. Cutting S., Panzer S., Losick R. 1989; Regulatory studies on the promoter for a gene governing synthesis and assembly of the spore coat in Bacillus subtilis.. J Mol Biol 107:393–404
    [Google Scholar]
  4. Cutting S., Oke V., Driks A., Losick R., Lu S., Kroos L. 1990; A forespore checkpoint for mother cell gene expression during development in B. subtilis.. Cell 62:239–250
    [Google Scholar]
  5. Cutting S., Driks A., Schmidt R., Kunkel B., Losick R. 1991a; Forespore-specific transcription of a gene in the signal transduction pathway that governs pro-σK processing in Bacillus subtilis.. Genes Dev 5:456–466
    [Google Scholar]
  6. Cutting S., Roels S., Losick R. 1991b; Sporulation operon spoIVF and the characterization of mutations that uncouple mother-cell from forespore gene expression in Bacillus subtilis.. J Mol Biol 221:1237–1256
    [Google Scholar]
  7. Errington J. 1993; Bacillus subtilis sporulation: regulation of gene expression and control of morphogenesis.. Microbiol Rev 57:0–33
    [Google Scholar]
  8. Gomez M., Cutting S.M. 1996; Expression of the Bacillus subtilis spoIVB gene is under dual σFG control.. Microbiology 142:3453–3457
    [Google Scholar]
  9. Gomez M., Cutting S.M. 1997; Identification of a new σB-controlled gene, csbX, in Bacillus subtilis.. Gene (in press)
    [Google Scholar]
  10. Gomez M., Cutting S., Stragier P. 1995; Transcription of spoIVB is the only role of σG that is essential for pro-σK processing during spore formation in Bacillus subtilis.. J Bacteriol 177:4825–4827
    [Google Scholar]
  11. Harwood C.R., Cutting S.M. (editors) 1990 Molecular Biology Methods for Bacillus. Chichester:: John Wiley.;
    [Google Scholar]
  12. Hoch J.A., Anagnostopoulos C. 1970; Chromosomal location and properties of radiation sensitivity mutations in Bacillus subtilis.. J Bacteriol 103:295–301
    [Google Scholar]
  13. Itaya M., Kondo K., Tanaka T. 1989; A neomycin resistance cassette selectable in a single state in the Bacillus subtilischromosome.. Nucleic Acids Res 17:4410
    [Google Scholar]
  14. Kenney T.J., Moran C.P. 1987; Organization and regulation of an operon that encodes a sporulation-essential sigma factor in Bacillus subtilis.. J Bacteriol 169:3329–3339
    [Google Scholar]
  15. Kunkel B., Kroos L., Poth H., Youngman P., Losick R. 1989; Temporal and spatial control of the mother-cell regulatory gene spoIIID of Bacillus subtilis.. Genes Dev 3:1735–1744
    [Google Scholar]
  16. Lu S., Halberg R., Kroos L. 1990; Processing of the mother-cell σ factor, σK, may depend on events occurring in the forespore during Bacillus subtilis development.. Proc Natl Acad Sci USA 879722–9726
    [Google Scholar]
  17. Lu S., Cutting S., Kroos L. 1995; Sporulation protein SpoIVFB from Bacillus subtilis enhances processing of the sigma factor precursor pro-σ-K in the absence of other sporulation gene products.. J Bacteriol 177:1082–1085
    [Google Scholar]
  18. Moran C.P. 1993; RNA polymerase and transcription factors.. In Bacillus subtilis and Other Grant-positive Bacteria pp. 653–667 Sonenshein A.L., Hoch J.A., Losick R. Edited by Washington, DC:: American Society for Microbiology.;
    [Google Scholar]
  19. Nicholson W.L., Setlow P. 1990; Sporulation, germination and outgrowth.. In Molecular Biological Methods for Bacillus pp. 391–450 Harwood C.R., Cutting S.M. Edited by Chichester:: John Wiley.;
    [Google Scholar]
  20. Oke V. 1994 Multilevel regulation of the sporulation transcription factor σK in Bacillus subtilis. PhD thesis Harvard University, Cambridge, MA.:
    [Google Scholar]
  21. Partridge S.R., Errington J. 1993; The importance of morphological events and intercellular interactions in the regulation of prespore-specific gene expression during sporulation in Bacillus subtilis.. Mol Microbiol 8:945–955
    [Google Scholar]
  22. Perego M. 1993; Integrational vectors for genetic manipulation in Bacillus subtilis. . In Bacillus subtilis and other Gram-positive bacteria pp. 615–624 Sonenshein A.L., Hoch J.A., Losick R. Edited by Washington, DC:: American Society for Microbiology.;
    [Google Scholar]
  23. Popham D.L., Stragier P. 1991; Cloning, characterization and expression of the spoVB gene of Bacillus subtilis.. J Bacteriol 173:7942–7949
    [Google Scholar]
  24. Resnekov O., Alper S., Losick R. 1996; Subcellular localization of proteins governing the proteolytic activation of a developmental transcription factor in Bacillus subtilis.. Genes to Cells 1:529–542
    [Google Scholar]
  25. Ricca E., Cutting S., Losick R. 1992; Characterization of bofA,a gene involved in intercompartmental regulation of pro-σK processing during sporulation in Bacillus subtilis.. J Bacteriol 174:3177–3184
    [Google Scholar]
  26. Sandman K., Kroos L., Cutting S., Youngman P., Losick R. 1988; Identification of the promoter for a spore coat protein gene in Bacillus subtilis and studies on the regulation of its induction at a late stage of sporulation.. J Mol Biol 200:461–473
    [Google Scholar]
  27. Schaeffer P., Millet J., Aubert J. 1965; Catabolic repression ofbacterial sporulation.. Proc Natl Acad Sci USA 54704–711
    [Google Scholar]
  28. Shazand K., Frandsen N., Stragier P. 1995; Cell-type specificity during development in Bacillus subtilis; the molecular and morphological requirements for σE activation.. EMBO J 14:1439–1445
    [Google Scholar]
  29. Shinagawa H., Makino K., Amemura M., Kimura S., Iwasaki H., Nakata A. 1988; Structure and regulation of the Escherichia coli ruv operon involved in DNA repair and recombination.. J Bacteriol 170:4322–4329
    [Google Scholar]
  30. Stragier P. 1992; Establishment of forespore-specific gene expression during sporulation of Bacillus subtilis. . In Prokaryotic Structure and Function: a New Perspective (Society for General Microbiology Symposium 47) pp. 297–310 Mohan S., Dow C., Cole J.A. Edited by Cambridge:: Cambridge University Press.;
    [Google Scholar]
  31. Stragier P., Bonamy C., Karmazyn-Campbelli C. 1988; Processing of a sporulation sigma factor in Bacillus subtilis: how morphological structure could control gene expression.. Cell 52:697–704
    [Google Scholar]
  32. Sun D., Setlow P. 1993; Cloning, nucleotide sequence, and regulation of the Bacillus subtilis nadB gene and a nifS-like gene, both of which are essential for NAD biosynthesis.. J Bacteriol 175:1423–1432
    [Google Scholar]
  33. Sun D., Stragier P., Setlow P. 1989; Identification of a new σ- factor involved in compartmentalized gene expression during sporulation of Bacillus subtilis.. Genes Dev 3:141–149
    [Google Scholar]
  34. Trach K., Hoch J.A. 1989; The Bacillus subtilis spoOB stage 0 sporulation operon encodes an essential GTP-binding protein.. J Bacteriol 171:1362–1371
    [Google Scholar]
  35. Youngman P., Perkins J., Losick R. 1984; Construction of a cloning site near one end of Tn917 into which foreign DNA may be inserted without affecting transposition in Bacillus subtilis or expression of the transposon-borne erm gene.. Plasmid 12:1–9
    [Google Scholar]
  36. Zheng L., Losick R. 1990; Cascade regulation of spore coat gene expression in Bacillus subtilis.. J Mol Biol 212:645–660
    [Google Scholar]
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