The photosynthetic protozoon Euglena gracilis Z contains adenosylcobalamin-dependent methylmalonyl-CoA mutase (MCM) involved in propionate metabolism. The specific activity of the Euglena mutase was about 6·5-fold greater in propionate-adapted Euglena cells than in photoautotrophic cells (control). Although the control cells contained only one mutase (apparent Mr 72000), the propionate-adapted cells contained two mutases with Mr values of 72000 and 17000; both enzymes were located in the mitochondria. These results provide evidence that propionate-adapted Euglena contains two MCM isozymes. The induced mutase (Mr 17000) permits photoassimilation of propionate.
BeaufayH., JacquesP., BaudhuinP., SellingerO. Z., BerthetJ., de DuveC.1964; Resolution of mitochondrial fractions from rat liver into three distinct populations of cytoplasmic particles by means of density equilibration in various gradients. Biochem J 92:184–205
BradfordM. M.1976; A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding. Anal Biochem 72:248–254
CannataJ. J. B., FocesiA.JrMazumderR., WarnerR. C., OchoaS.1965; Metabolism of propionic acid in animal tissues. XII. Properties of mammalian methylmalony] coenzyme A mutase. J Biol Chem 240:3249–3257
de DuveD., PressmanB. C., GianettoR., WattiauxT. G., ApplemansF.1955; Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat liver. Biochem J 60:604–617
FentonW. A., HackA. M., WillardM. F., GertlerA., RosenbergL. E.1982; Purification and properties of methylmalonvl coenzyme A mutase from human liver. Arch Biochem Biophys 214:815–823
FrancalanciF., DavisN. K., FullerJ. Q., MurfittD., LeadlayP. F.1986; The subunit structure of methylmalonyl-CoA mutase from Propionibacterium shermanii.. Biochem J 236:489–494
IsegawaY., NakanoY., KitaokaS.1984; Conversion and distribution of cobalamin in Euglena gracilis z, with special reference to its location and probable function within chloroplasts. Plant Physiol 76:814–818
IsegawaY., WatanabeF., KitaokaS., NakanoY.1994; Subcellular distribution of cobalamin-dependent methionine synthase in Euglena gracilis z. Phytochemistry 35:59–61
KitaokaS., NakanoY., MiyatakeK., YokotaA.1989; Enzymes and their functional Location. In The Biology of Euglena4 pp. 1–135BuetowD. E. Edited by San Diego: Academic Press;
RosenbergL. E., FentonW. A.1989; Disorders of propionate and methylmalonate metabolism.. In The Metabolic Basis of Inherited Disease pp. 822–844ScriverC. R., BeaudetW. S., Sly, ValleD. Edited by New York: McGraw Hill;
TokunagaM., NakanoY., KitaokaS.1976; Separation and properties of the NAD-linked and NADP-linked isozymes of succinic semialdehyde dehydrogenase in Euglena gracilis z. Biochim Biophys Acta 429:55–62
WatanabeF., NakanoY., KitaokaS.1987b; Isolation and some properties of soluble and membrane-bound cobalamin binding proteins of Euglenamitochondria. Arch Microbiol 149:30–35
WatanabeF., NakanoY., KitaokaS.1987c; Purification and some properties of cytosolic cobalamin-binding protein m Euglena gracilis.. Biochem J 247:679–685
WatanabeF., ItoT., TabuchiT., NakanoY., KitaokaS.1988a; Isolation of pellicular cobalamin-binding proteins of the cobalamin uptake system of Euglena gracilis.. J Gen Microbiol 134:67–74
WatanabeF., NakanoY., OchiH., KitaokaS. . 1988b; Purification, stime properties and possible physiological role of an extracellular cobalamin binding protein from Euglena gracilis.. J Gen Microbiol 134:1385–1389
WatanabeF., NakanoY., StupperichE.1992; Different corrinoid specificities for cell growth and the cobalamin uptake system in Euglena gracilis z. J Gen Microbiol 138:1807–1813
WatanabeF., NakanoY., TamuraT., StupperichE.1993b; Corrinoid specificity of cytosolic cobalamin-binding protein of Euglena gracilis z. J Biochem 113:97–100
WatanabeF., NakanoY., StupperichE., UshikoshiK., UshikoshiS., UshikoshiI., KitaokaS.1993c; A radioisotope dilution method for quantitation of total vitamin B12 in biological sample using isolated Euglena pellicle fragments as a solid-phase vitamin B12-binding material. Anal Chem 65:657–659
YokotaA., HosotaniK., KitaokaS.1982; Mechanism of metabolic regulation in photoassimilation of propionate in Euglena gracilis z. Arch Biochem Biophys 249:530–537