1887

Abstract

The photosynthetic protozoon Z contains adenosylcobalamin-dependent methylmalonyl-CoA mutase (MCM) involved in propionate metabolism. The specific activity of the mutase was about 6·5-fold greater in propionate-adapted cells than in photoautotrophic cells (control). Although the control cells contained only one mutase (apparent 72000), the propionate-adapted cells contained two mutases with values of 72000 and 17000; both enzymes were located in the mitochondria. These results provide evidence that propionate-adapted contains two MCM isozymes. The induced mutase ( 17000) permits photoassimilation of propionate.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-142-9-2631
1996-09-01
2022-01-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/142/9/mic-142-9-2631.html?itemId=/content/journal/micro/10.1099/00221287-142-9-2631&mimeType=html&fmt=ahah

References

  1. Beaufay H., Jacques P., Baudhuin P., Sellinger O. Z., Berthet J., de Duve C. 1964; Resolution of mitochondrial fractions from rat liver into three distinct populations of cytoplasmic particles by means of density equilibration in various gradients. Biochem J 92:184–205
    [Google Scholar]
  2. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding. Anal Biochem 72:248–254
    [Google Scholar]
  3. Cannata J. J. B., Focesi A.Jr Mazumder R., Warner R. C., Ochoa S. 1965; Metabolism of propionic acid in animal tissues. XII. Properties of mammalian methylmalony] coenzyme A mutase. J Biol Chem 240:3249–3257
    [Google Scholar]
  4. Cramer M. C., Myers J. 1952; Growth and photosynthetic characteristics of Euglena gracilis.. Arch Mikrobiol 17:384–402
    [Google Scholar]
  5. de Duve D., Pressman B. C., Gianetto R., Wattiaux T. G., Applemans F. 1955; Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat liver. Biochem J 60:604–617
    [Google Scholar]
  6. Fenton W. A., Hack A. M., Willard M. F., Gertler A., Rosenberg L. E. 1982; Purification and properties of methylmalonvl coenzyme A mutase from human liver. Arch Biochem Biophys 214:815–823
    [Google Scholar]
  7. Francalanci F., Davis N. K., Fuller J. Q., Murfitt D., Leadlay P. F. 1986; The subunit structure of methylmalonyl-CoA mutase from Propionibacterium shermanii.. Biochem J 236:489–494
    [Google Scholar]
  8. Hamilton F. D. 1974; Ribonucleotide reductase from Euglena gracilis: a 5′-deoxyadenosylcobalamin-dependent enzyme. J Biol Chem 249:4428–4434
    [Google Scholar]
  9. Isegawa Y., Nakano Y., Kitaoka S. 1984; Conversion and distribution of cobalamin in Euglena gracilis z, with special reference to its location and probable function within chloroplasts. Plant Physiol 76:814–818
    [Google Scholar]
  10. Isegawa Y., Watanabe F., Kitaoka S., Nakano Y. 1994; Subcellular distribution of cobalamin-dependent methionine synthase in Euglena gracilis z. Phytochemistry 35:59–61
    [Google Scholar]
  11. Kitaoka S., Nakano Y., Miyatake K., Yokota A. 1989; Enzymes and their functional Location. In The Biology of Euglena 4 pp. 1–135 Buetow D. E. Edited by San Diego: Academic Press;
    [Google Scholar]
  12. Koren L. E., Hutner S. H. 1967; High-yield media for photo-synthesizing Euglena gracilis z.. J Protocol 14S:17
    [Google Scholar]
  13. Rabinowitz H., Reisfeld A., Sagher D., Edelman M. 1975; Ribulose diphosphate carboxylase from autotrophic Euglena gracilis.. Plant Physiol 56:345–350
    [Google Scholar]
  14. Rosenberg L. E., Fenton W. A. 1989; Disorders of propionate and methylmalonate metabolism.. In The Metabolic Basis of Inherited Disease pp. 822–844 Scriver C. R., Beaudet W. S., Sly, Valle D. Edited by New York: McGraw Hill;
    [Google Scholar]
  15. Tokunaga M., Nakano Y., Kitaoka S. 1976; Separation and properties of the NAD-linked and NADP-linked isozymes of succinic semialdehyde dehydrogenase in Euglena gracilis z. Biochim Biophys Acta 429:55–62
    [Google Scholar]
  16. Tokunaga M., Nakano Y., Kitaoka S. 1979; Subcellular location of the GABA-shunt enzymes in Euglena gracilisstrain Z. J Protozool 26:471–473
    [Google Scholar]
  17. Watanabe F., Nakano Y., Kitaoka S. 1987a; Purification and characterization of aquacobalamin reductase (NADPH) from Euglena gracilis.. J Biol Chem 262:11514–11518
    [Google Scholar]
  18. Watanabe F., Nakano Y., Kitaoka S. 1987b; Isolation and some properties of soluble and membrane-bound cobalamin binding proteins of Euglenamitochondria. Arch Microbiol 149:30–35
    [Google Scholar]
  19. Watanabe F., Nakano Y., Kitaoka S. 1987c; Purification and some properties of cytosolic cobalamin-binding protein m Euglena gracilis.. Biochem J 247:679–685
    [Google Scholar]
  20. Watanabe F., Ito T., Tabuchi T., Nakano Y., Kitaoka S. 1988a; Isolation of pellicular cobalamin-binding proteins of the cobalamin uptake system of Euglena gracilis.. J Gen Microbiol 134:67–74
    [Google Scholar]
  21. Watanabe F., Nakano Y., Ochi H., Kitaoka S. . 1988b; Purification, stime properties and possible physiological role of an extracellular cobalamin binding protein from Euglena gracilis.. J Gen Microbiol 134:1385–1389
    [Google Scholar]
  22. Watanabe F., Nakano Y., Stupperich E. 1992; Different corrinoid specificities for cell growth and the cobalamin uptake system in Euglena gracilis z. J Gen Microbiol 138:1807–1813
    [Google Scholar]
  23. Watanabe F., Nakano Y., Stupperich E. 1993a; Uptake of cobalamin by Euglena mitochondria. . J Biochem 114:793–799
    [Google Scholar]
  24. Watanabe F., Nakano Y., Tamura T., Stupperich E. 1993b; Corrinoid specificity of cytosolic cobalamin-binding protein of Euglena gracilis z. J Biochem 113:97–100
    [Google Scholar]
  25. Watanabe F., Nakano Y., Stupperich E., Ushikoshi K., Ushikoshi S., Ushikoshi I., Kitaoka S. 1993c; A radioisotope dilution method for quantitation of total vitamin B12 in biological sample using isolated Euglena pellicle fragments as a solid-phase vitamin B12-binding material. Anal Chem 65:657–659
    [Google Scholar]
  26. Watanabe F., Tamura Y., Saido H., Nakano Y. 1993d; Enzymatic assay for adenosylcobalamin-dependent methylmalony] coenzyme A mutase. Biosci Biotechnol Biochem 57:1593–1594
    [Google Scholar]
  27. Yokota A., Hosotani K., Kitaoka S. 1982; Mechanism of metabolic regulation in photoassimilation of propionate in Euglena gracilis z. Arch Biochem Biophys 249:530–537
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-142-9-2631
Loading
/content/journal/micro/10.1099/00221287-142-9-2631
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error