1887

Abstract

A physical and rudimentary genetic map of the strain 35000 genome was constructed. Pulsed-field gel electrophoresis was used to separate restriction fragments of DNA digested with I, I-l, or l plus I-Ceul. The sizes of the fragments were determined, and the circular chromosome was estimated to be 1757 kbp. The six I-l fragments and four l fragments were ordered into macrorestriction maps using Southern blot hybridization with random clones as probes. It was shown that both and the distantly related have six operons marked by the locations of the I-l sites. However, the two species displayed distinct I-I restriction patterns. A second strain, CIP542, displayed an identical I-I pattern to that of 35000, but I digests of the two strains were distinct. The orientation of the six operons was determined and thirteen identified genes positioned on the map of strain 35000.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-142-9-2587
1996-09-01
2021-07-30
Loading full text...

Full text loading...

/deliver/fulltext/micro/142/9/mic-142-9-2587.html?itemId=/content/journal/micro/10.1099/00221287-142-9-2587&mimeType=html&fmt=ahah

References

  1. Alfa M. J. 1992; Cytopathic effect of Haemophilus ducreyi for human foreskin cell culture. J Med Microbiol 37:43–50
    [Google Scholar]
  2. Bachmann B. J. 1990; Linkage map of Escherichia coli K-12, edition 8. Microbiol Rep 54:130–197
    [Google Scholar]
  3. Biegel S. D., Thomas C. E., Elkins C. 1995; Cloning and sequencing of a Haemophilus ducreyi fur homolog. In Abstracts of the 95th General Meeting of the American Society for Microbiology, Abstract B-354. Washington: American Society for Microbiology;
    [Google Scholar]
  4. Brentjens R., Ketterer M., Apicella M. A., Spinola S. M. 1996; Fine tangled pili expressed by Haemophilus ducreyi are a novel class of pili. J Bacteriol 178:808–816
    [Google Scholar]
  5. Brosius J., Dull T. J., Sleeter D. D., Noller H. F. 1981; Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. J Mol Biol 148:107–127
    [Google Scholar]
  6. Butler P. D., Moxon E. R. 1990; A physical map of the genome of Haemophilus influenzae type b. J Gen Microbiol 136:2333–2342
    [Google Scholar]
  7. Campagnari A. A., Wild L. M., Griffiths G. E., Karalus R. J., Wirth M. A., Spinola S. M. 1991; Role of lipooligosaccharides in experimental dermal lesions caused by Haemophilus ducreyi. Infect Immum 59:2601–2608
    [Google Scholar]
  8. Castellazzo A., Shero M, Apicella M. A., Spinola S. M. 1992; Expression of pili bv Haemophilus ducreyi. J Infect Dis 165:S198–S199
    [Google Scholar]
  9. Dempsey J. A., Litaker W., Madhure A., Snodgrass T. L., Cannon J. G. 1991; Physical map of the chromosome of Neisseria gonorrhoeae FA1090 with locations of genetic markers, including opa and pil genes. J Bacteriol 173:5476–5486
    [Google Scholar]
  10. Deneer H. G., Slaney L., MacLean I. W., Albritton W. L. 1982; Mobilization of noeconjugative antibiotic resistance plasmids in Haemophilus ducreyi. J Bacterial 149:726–732
    [Google Scholar]
  11. Dewhirst F. E., Paster B. J., Olsen I., Fraser G. J. 1992; Phylogeny of 54 representative strains of species in the family Pasteurellaceae as determined by comparison of 16S rRNA sequences. J Bacteriol 174:2002–2013
    [Google Scholar]
  12. Duester G. L., Holmes W. M. 1980; The distal end of the ribosomal RNA operon rrnD of Escherichia cali contains a tRNAThrl gene, two 5S rRNA genes and a transcription terminator. Nucleic Acids Res 8:3793–3807
    [Google Scholar]
  13. Elkins C., Chen C. J., Thomas C. E. 1995; Characterization of the hghA locus encoding a hemoglobin receptor from Haemophilus ducreyi. Infect Immun 63:2194–2200
    [Google Scholar]
  14. Fleischmann R. D., Adams M. D., White O., Clayton R. A., Kirkness E. F., Kerlavage A. R., Bult C. J., Tomb J.-F., Dougherty B. A., Merrick J. M., McKenney K., Sutton G., FitzHugh W., Fields C., Gocayne J. D., Scott J., Shirley R., Liu L.-I., Glodek A., Kelley J. M., Weidman J. F., Phillips C. A., Spriggs T., Hedblom E., Cotton M. D., Utterback T. R., Hanna M.C, Nguyen D. T., Saudek D. M., Brandon R. C., Fine L. D., Fritchman J. L., Furhmann J. L., Geoghagen N. S. M., Gnehm C. S., McDonald L. A., Small K. V., Fraser C. M., Smith H. O., Venter J. C. 1995; Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science 269:496–512
    [Google Scholar]
  15. Fonstein M., Haselkorn R. 1995; Physical mapping of bacterial genomes. J Bacteriol 177:3361–3369
    [Google Scholar]
  16. Fortney K., Hiltke T., Shanks K., Spinola S. 1996; The conserved 18,000 molecular weight outer membrane protein of Haemophilus ducreyi has homology to PAL. . In Abstracts of the 96th General Meeting of the American Society for Microbiology, Abstract B-319. Washington: American Society for Microbiology;
    [Google Scholar]
  17. Hammond G. W., Lian C. J., Wilt J. C., Roland A. R. 1978; Antimicrobial susceptibility of Haemophilus ducreyi. Antimicrob Agents Chemother 7:39–43
    [Google Scholar]
  18. Hiltke T., Spinola S. 1995; Cloning of the gene encoding the major outer membrane protein of Haemophilus ducreyi. In Abstracts of the 95th General Meeting of the American Society for Aiicrohiology, Abstract B-189. Washington: American Society for Microbiology;
    [Google Scholar]
  19. Hiltke T., Lau G, Curunathan S., Juarez Z., Campagnari A., Spinola S. 1994; Cloning of a gene encoding a 28 kilodalton outer membrane protein of Haemophilus ducreyi. In Abstracts of the 94th General Meeting of the American Society for Microbiology,Abstract B-154. Washington: American Society for Microbiology;
    [Google Scholar]
  20. Hobbs M. M., San Mateo L. R., Orndorff P. E., Almond G., Kawula T. H. 1995; Swine model of Haemophilus ducreyi infection. Infect Immun 63:3094–3100
    [Google Scholar]
  21. Hollyer T. T., DeGagne P. A., Alfa M. J. 1994; Characterization of the cvtopathic effect of Haemophilus ducreyi. Sex Transm Dis 21:247–257
    [Google Scholar]
  22. Honeycutt R. J., McClelland M., Sobral B. W. 1993; Physical map ot the genome of Rhizobium meliloti 1021. J Bacteriol 175:6945–6952
    [Google Scholar]
  23. Jinks-Robertson S., Nomura M. 1987; Ribosomes and tRNA. In Escherichia coli and Salmonella typhimurium Cellular and Molecular Biology pp. 1358–1379 Neidhardt F. C. Edited by Washington: American Society for Microbiology;
    [Google Scholar]
  24. Kauc L., Goodgal S. H. 1989; The size and a physical map of the chromosome of Haemophilus parainfluenzae. Gene 83:377–380
    [Google Scholar]
  25. Kilian M., Biberstein E. L. 1984; Genus II.Haemophilus. In Bergey̓s Manual of Systematic Bacteriology 1 pp. 558–569 Kreig N. R., Holt J. G. Edited by Baltimore: Williams & Wilkins;
    [Google Scholar]
  26. Lammel C. J., Dekker N. P., Palefsky J., Brooks G. F. 1993; In vitro model of Haemophilus ducreyi adherence to and entry into eukaryotic cells of genital origin. J Infect Dis 167:642–650
    [Google Scholar]
  27. Le-Bacq F., Mason P. R., Gwanzura L., Robertson V. J., Latif A. S. 1993; HIV and other sexually transmitted diseases at a rural hospital in Zimbabwe. Genitourin Med 69:352–356
    [Google Scholar]
  28. Lee J. J., Smith H. O., Redfield R. J. 1989; Organization of the Haemophilus influenzae Rd genome. J Bacteriol 171:3016–3024
    [Google Scholar]
  29. Liu S.-L., Hessel A., Sanderson K. E. 1993; Genomic mapping with 1-CeuI, an intron-encoded endonuclease specific for genes for ribosomal RNA, in Salmonellaspp., Escherichia coli, and other bacteria. Proc Natl Acad Sci USA 906874–6878
    [Google Scholar]
  30. Musialowski M. S., Flett F., Scott G. B., Hobbs G., Smith C. P., Oliver S. G. 1994; Functional evidence that the principal DNA replication origin of the Streptomyces coelicolor chromosome is close to the dnaA-gyrB region. J Bacteriol 176:5123–5125
    [Google Scholar]
  31. Nzila N., Laga M., Thiam M. A., Mayimona K., Edidi B., Van-Dyck E., Behets F., Hassig S., Nelson A., Mokwa K., Ashley R. L., Piot P., Ryder R. W. 1991; HIV and other sexually transmitted diseases among female prostitutes in Kinshasa. AIDS 5:715–721
    [Google Scholar]
  32. Ogasawara N., Moriya R., von Meyenberg K., Hansen F. G., Yoshikawa H. 1985; C lonservation of genes and their organization in the chromosomal replication of Bacillus subtilis and Escherichia coli. EMBO J 4:3345–3350
    [Google Scholar]
  33. Palmer K. L., Munson R. S.Jr 1995; Cloning and characterization of the genes encoding the hemolysin of Haemophilus ducreyi. Mol Microbiol (in press)
    [Google Scholar]
  34. Palmer K. L., Grass S., Munson R. S.Jr 1994; Identification of a hemolytic activity elaborated by Haemophilus ducreyi.. Infect Immun 62:3041–3043
    [Google Scholar]
  35. Parsons L.M-, Waring A. L., Shayegani M. 1992; Molecular analysis of the Haemophilus ducreyi groE heat shock operon. Infect Immun 60:4111–4118
    [Google Scholar]
  36. Parsons L. M., Waring A. L., Limberger R. J., Shayegani M. 1995; Effect of increased expression of DnaK (HSP70) in Haemophilus ducreyi. In Abstracts of the 95th General Meeting of the American Society for Microbiology, Abstract D-82. Washington: American Society for Microbiology;
    [Google Scholar]
  37. Pepin J., Quigley M., Todd J., Gaye I., Janneh M., Van Dyck E., Piot P., Whittle H. 1992; Association between HIV-2 infection and genital ulcer diseases among male sexually transmitted disease patients in The Gambia. AIDS 6:489–493
    [Google Scholar]
  38. Plummer F. A., Simonsen J. N., Cameron D. W., Ndinya-Achola J. O., Kreiss J. K., Gakinya M. N., Waiyaki P., Cheang M., Piot P., Ronald A. R., Ngugi E. N. 1991; Cofactors in male-female sexual transmission of human immunodeficiency virus type 1. J Infect Dis 163:233–239
    [Google Scholar]
  39. Purcell B. K., Richardson J. A., Radolf J. D., Hansen E. J. 1991; A temperature-dependent rabbit model for production of dermal lesions by Haemophilus ducreyi. J Infect Dis 164:359–367
    [Google Scholar]
  40. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Eaboratory Alanual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  41. San Mateo LR., Hobbs M. M., Kawula T. H. 1995; Haemophilus ducreyi produces a copper-zinc superoxide dismutase. In Abstracts of the 95th General Sleeting of the American Society for Microbiology, Abstract B-190. Washington: American Society for Microbiology;
    [Google Scholar]
  42. Skerman V. B. D., McGowan V., Sneath P. H. A. 1980; Approved Lists of Bacterial Names. Int J Syst Bacteriol 30:225–420
    [Google Scholar]
  43. Spinola S. M., Castellazzo A., Shero M., Apiceila M. A. 1990; Characterization of pili expressed by Haemophilus ducreyi. Microb Pathog 9:417–426
    [Google Scholar]
  44. Spinola S. M., Griffiths G. E., Bogdan J., Menegus M. A. 1992; Characterization of an 18,000-molecular-weight outer membrane protein of Haesmophilus ducreyi that contains a conserved surface-exposed epitope. Infect Immun 60:385–391
    [Google Scholar]
  45. Spinola S. M., Griffiths G. E., Shanks K. L., Blake M. S. 1993; The major outer membrane protein of Haemophilus ducreyi is a member of the OmpA family of proteins. Infect Immun 61:1346–1351
    [Google Scholar]
  46. Spinola S. M., Wild L. M., Apicella M. A., Gaspari A. A., Campagnari A. A. 1994; Experimental human infection with Haemophilus ducreyi. J Infect Dis 169:1146–1150
    [Google Scholar]
  47. Stein D.G, Danaher R. J., Cook T. M. 1991; Characterization of a gyrB mutation responsible for low-level nalidixic acid resistance in Neisseria gonorrhoeae. Antimicrob Agents Chemother 35:622–626
    [Google Scholar]
  48. Totten P. A., Morton W. R., Knitter G. H., Clark A. M., Kiviat N. B., Stamm W. E. 1994; A primate model for chancroid. J Infect Dis 169:1284–1290
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-142-9-2587
Loading
/content/journal/micro/10.1099/00221287-142-9-2587
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error