The gene for γ-glutamylcysteine synthetase () from was isolated from a family of cosmids by its ability to complement an double mutant which was unable to grow on minimal medium lacking glutathione. The predicted sequence of the γ-glutamylcysteine synthetase was found to have only 18% amino acid sequence identity to the equivalent enzyme from In spite of this low sequence homology, concentrations of GSH in a cell extract prepared from the mutant containing the cloned gene were almost as high as in a cell extract prepared from a wild-type strain. The gene was found to be physically and transcriptionally linked to the gene for citrate synthase (). The and citrate synthases shared 37% amino acid sequence identity and the cloned citrate synthase gene was able to complement an mutant.


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