1887

Abstract

The gene for -glutamylcysteine synthetase () from was isolated from a family of cosmids by its ability to complement an double mutant which was unable to grow on minimal medium lacking glutathione. The predicted sequence of the -glutamylcysteine synthetase was found to have only 18% amino acid sequence identity to the equivalent enzyme from . In spite of this low sequence homology, concentrations of GSH in a cell extract prepared from the mutant containing the cloned gene were almost as high as in a cell extract prepared from a wild-type strain. The gene was found to be physically and transcriptionally linked to the gene for citrate synthase (). The and citrate synthases shared 37% amino acid sequence identity and the cloned citrate synthase gene was able to complement an mutant.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-142-9-2543
1996-09-01
2021-05-14
Loading full text...

Full text loading...

/deliver/fulltext/micro/142/9/mic-142-9-2543.html?itemId=/content/journal/micro/10.1099/00221287-142-9-2543&mimeType=html&fmt=ahah

References

  1. Aiba H., Adhaya S., de Crombrugge B. 1981; Evidence for two functional gal promoters in intact E.coli cells. J Biol Chem 256:11905–11910
    [Google Scholar]
  2. Clennel A.-M., Johnston B., Rawlings D. E. 1995; Structure and function of Tn 5467, a Tn 21-like transposon located on the Thiobacillus ferrooxidans broad-host-range plasmid pTF-FC2. Appl Environ Microbiol 61:4223–4229
    [Google Scholar]
  3. Dennda G., Kula M.-R. 1986; Purification and evaluation of the glutathione-synthesizing enzymes of Candida boidinii for cell-free synthesis of glutathione. J Biotechnol 4:143–158
    [Google Scholar]
  4. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12:387–395
    [Google Scholar]
  5. Hell R., Bergmann L. 1990; γ-Glutamylcvsteine synthetase in higher plants: catalytic properties and subcellular localization. Planta 180:603–612
    [Google Scholar]
  6. Hissin P. J., Hilf R. 1976; A fluorometric method for determination of oxidized and reduced glutathione in tissues. Anal Biochem 74:214
    [Google Scholar]
  7. Huang C.-S., Moore W. R., Meister A. 1988; On the active site thiol of ᵞ-glutamylcysteine synthetase: relationships to catalysis, inhibition, and regulation. Proc Nall Acad Sci USA 852464–2468
    [Google Scholar]
  8. Huang C. S., Anderson M. E., Meister A. 1993; Amino acid sequence and function of the light subunit of rat kidney gamma- glutamylcysteine synthetase. J Biol Chem 268:20578–20583
    [Google Scholar]
  9. Kumagai H., Nakayama R., Tochikura T. 1982; γ-Glutamyl-cysteine synthetase from Proteus mirabilis. Agric Biol Chem 46:1301–1309
    [Google Scholar]
  10. Lim C.-J., Gleason F. K., Fuchs J. A. 1986; Cloning, expression and characterization of the Anabaena thioredoxin gene is Escherichia coli. J Bacteriol 168:1258–1264
    [Google Scholar]
  11. Lindström E. B., Gunneriusson E., Tuovinen O. H. 1992; Bacterial oxidation of refractory sulfide ores for gold recovery. Crit Rev Biotechnol 12:133–155
    [Google Scholar]
  12. May M. J., Leaver C. J. 1994; Arabidopsis tbaliana γ-glutamylcysteine synthetase is structurally unrelated to mammalian, yeast, and Escherichia coli homologs. Proc Natl Acad Sci USA 9110059–10063
    [Google Scholar]
  13. Meister A., Anderson M. E. 1983; Glutathione. Annu Rev Biochem 52:711–760
    [Google Scholar]
  14. Powles R. E., Deane S. M., Rawlings D. E. 1995; Molecular and genetic analysis of a thioredoxin gene from Thiobacillus ferrooxidans. Microbiology 141:2175–2181
    [Google Scholar]
  15. Ramesar R. S. 1988 Developmental genetic studies on Thiobacillus ferrooxidans. PhD thesis, University of Cape Town, South Africa
    [Google Scholar]
  16. Richman P. G., Meister A. 1975; Regulation of γ-glutamylcysteine synthetase by nonallosteric feedback inhibition by glutathione. J Biol Chem 250:1422–1426
    [Google Scholar]
  17. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning : a Eaboratory Manual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  18. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 743650–3654
    [Google Scholar]
  19. Seelig G. F., Simondsen R. P., Meister A. 1984; Reversible dissociation of γ-glutamvlcysteine synthetase into two subunits. J Biol Chem 259:9345–9347
    [Google Scholar]
  20. Wood D. O., Williamson L. R., Winkler H. H., Krause D. C. 1987; Nucleotide sequence of the Rickettsia prowazekii citrate synthase gene. J Bacteriol 169:3564–3572
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-142-9-2543
Loading
/content/journal/micro/10.1099/00221287-142-9-2543
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error