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Volume 142, Issue 9

Isolation of genes encoding homologues of DNA-binding protein HU and ribosomal protein S20 Free

Abstract

Linear DNA with covalently closed ends is the predominant form of DNA in the spirochaete . All bacteria examined to date have small DNA-binding proteins related to the IHF and HU proteins that appear to play roles in DNA compaction and replication, but such proteins had not been isolated from bacteria with linear genomes. We found a single gene in (named ) whose product (named Hbb) complements the defects for λ DNA packaging found in strains mutant in the genes for IHF and HU. The sequence of the predicted protein is similar to those of HU and IHF-like proteins in other bacteria. The gene appears to be in an operon with the order where the gene is a homologue of the gene encoding ribosomal protein S20 and the gene encodes a protein of unknown function. This operon is located upstream of the previously identified homologue.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Borrelia burgdorferi , HU protein , integration host factor (IHF) and Lyme disease
© Society for General Microbiology 1996
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1996-09-01
2024-04-25
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Isolation of Borrelia burgdorferi genes encoding homologues of DNA-binding protein HU and ribosomal protein S20
Microbiology 142, 2471 (1996); https://doi.org/10.1099/00221287-142-9-2471
/content/journal/micro/10.1099/00221287-142-9-2471
/content/journal/micro/10.1099/00221287-142-9-2471
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