An insertion sequence (IS) of has been isolated and sequenced. This 1323 bp element, designated IS is present in up to 18 copies in the genome and may be harboured in an extra-chromosomal element. It encodes a putative transposase of 415 amino acids which displays sequence homology to the IS family. Members of this class of elements have been described in the genus - for example IS is present in the complex, IS-IS in and IS in these elements exhibit an 89%, 45% and 16% amino acid identity with IS respectively. Investigation of the host range of IS by Southern blot analysis revealed additional IS-related repeated sequences in and Moreover, IS represents a useful epidemiological tool for strain typing as it provides a diversity of restriction fragment length polymorphism patterns.


Article metrics loading...

Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error