1887

Abstract

An insertion sequence (IS) of has been isolated and sequenced. This 1323 bp element, designated IS, is present in up to 18 copies in the genome and may be harboured in an extra-chromosomal element. It encodes a putative transposase of 415 amino acids which displays sequence homology to the IS family. Members of this class of elements have been described in the genus - for example IS is present in the complex, IS-IS in , and IS in these elements exhibit an 89%, 45% and 16% amino acid identity with IS, respectively. Investigation of the host range of IS by Southern blot analysis revealed additional IS-related repeated sequences in and . Moreover, IS represents a useful epidemiological tool for strain typing as it provides a diversity of restriction fragment length polymorphism patterns.

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1996-09-01
2021-10-20
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