We have found a new insertion sequence (IS), designated IS Aa1, downstream of the S10 operon in Actinobacillus (Haemophilus) actinomycetemcomitans FDC Y4. IS Aa1, the first IS element characterized in this organism, is 705 bp long and lacks terminal inverted repeats. This element displayed significant homology with IS200. Hybridization patterns of genomic DNA of seven A. actinomycetemcomitans strains with an internal ISAa1 probe varied depending on the serotypes, suggesting that IS Aa1 might be a useful tool for epidemiological studies.
BisercicM.,
OchmanH.1993a; Natural populations of Escherichia coli and Salmonella typhimurium harbor the same classes of insertion sequences. Genetics 133:449–454
BrynestadS.,
IwanejkoL. A.,
StewartG.S.A.B.,
GranumP. E.1994; A complex array of Hpr consensus DNA recognition sequences proximal to the enterotoxin gene in Clostridium perfringens type A. Microbiology 140:97–104
DewhirstF. E.,
PasterB. J.,
OlsenI.,
FraserG. J.1992; Phylogeny of 54 representative strains of species in the family P asteurellaceae as determined by comparison of 16S rRNA sequences. J Bacteriol 174:2002–2013
GalasD. J.,
ChandlerM.1989; Bacterial insertion sequences. In Mobile DNA pp. 109–162BergD. E.,
HoweM. M.
Edited by Washington, DC: American Society for Microbiology.;
HanN.,
HooverC. I.,
WinklerJ. R.,
NgC. Y.,
ArmitageG. C.1991; Identification of genomic clonal types of Actimbacillus actinomycetemcomitans by restriction endonuclease analysis. J Clin Microbiol 29:1574–1578
HermansP. W. M.,
van SoolgenD.,
DaleJ. W.,
SchutemaA. R. J.,
McAdamP. A.,
CattyD.,
van EmbdenJ. D. A.1990; Insertion element IS 986 from Mycobacterium tuberculosis:a useful tool for diagnosis and epidemiology of tuberculosis. J Clin Microbiol 28:2051–2058
KromerW. J.,
HatakeyamaT.,
KimuraM.1990; Nucleotide sequence of Bacillus stearothermophilus ribosomal protein genes: part of the ribosomal S10 operon. Biol Chem Hoppe-Seyler 371:631–636
O´ReillyC.,
BlackG. W.,
LaffeyR.,
McConnellD. J.1990; Molecular analysis of an IS200 insertion in the gpt gene of Salmonella typhimurium LT2. J Bacteriol 172:6599–6601
PhillipsJ. E.1984; Genus III. Actinobacillus. In Bergey’s Manual of Systematic Bacteriology1 pp. 570–575KriegN. R.,
HoltJ. G.
Edited by Baltimore:: Williams & WTllkins.;
PoulsenK.,
TheiladeE.,
LallyE. T.,
DemuthD. R.,
KilianM.1994; Population structure of Actinobacillus actinomycetemcomitans: a framework for studies of disease-associated properties. Microbiology 140:2049–2060
SimonetM.,
RiotB.,
FortimeauN.,
BercheB.1996; Invasin production by Yersinia pestis is abolished by insertion of an IS200-like element within the inv gene. Infect Immun 64:375–379
Van SteenbergenT. J. M.,
van der VerdenU.,
AbbasF.,
de GraaffJ.1991; Microflora and bacterial DNA restriction enzyme analysis in young adults with periodontitis. J Periodontol 62:235–241
Van SteenbergenT.J.M,
Bosch-TijhojC. J.,
van WinkelhoffA. J.,
GmurR.,
de GraaffJ.1994; Comparison of six typing methods for Actinobacillus actinomycetemcomitans. J Clin Microbiol 32:2769–2774