1887

Microbiology Society logo

Volume 142, Issue 9

Curing of F-like plasmid TP181 by plumbagin is due to interference with both replication and maintenance functions Free

Abstract

F-like plasmid TP181 is particularly susceptible to curing by the naphthoquinone derivative plumbagin, which may attack DNA gyrase. TP181 should provide particular insight into the basis of plasmid elimination, which has application in a number of contexts. Curing was found to be optimal at pH 7·2. An Rl fragment containing the RF1A replicon of TP181 was joined to a Km determinant (giving miniTP181). MiniTP181 had the same increased susceptibility to curing by plumbagin when compared to miniF as TP181 had relative to F. Plumbagin interfered with replication of miniTP181, depressing its copy number and increasing the rate of segregation. Plumbagin also blocked the lethal effect of the locus after rifampicin treatment, which mimics production of plasmid-free segregants, so that more of these plasmid-free cells would survive. Restriction mapping and DNA sequence analysis indicated that the locus of TP181 is almost identical to that of F but that TP181 lacks the gene present in F which is needed to activate replication from . Thus the sensitivity of TP181 may be due to its dependence on both a replicon which is hypersensitive to perturbation of supercoiling by plumbagin and a host-killing system which is blocked by plumbagin.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): ccd locus , plasmid curing , plumbagin and rifampicin
© Society for General Microbiology 1996
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-142-9-2399
1996-09-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/142/9/mic-142-9-2399.html?itemId=/content/journal/micro/10.1099/00221287-142-9-2399&mimeType=html&fmt=ahah

References

  1. Austin S., Wierzbiecki A. 1983; Two mini-F encoded proteins are essential for equipartitioning. Plasmid 10:73–81
     [Google Scholar]
  2. Bernard P., Couturier M. 1992; Cell killing by F plasmid ccdBinvolves poisoning of DNA topoisomerase II complexes. J Mol Biol 226:735–745
     [Google Scholar]
  3. Bernard P., Kezdy K. E., Meldreren L. V., Steyaert J., Wyns L., Pato M. L., Higgins P., Couturier M. 1993; The F plasmid ccdB protein induces efficient ATP dependent DNA cleavage by gyrase. J Mol Biol 234:534–541
     [Google Scholar]
  4. Bergquist P. L., Lane H. E. D., Malcolm L., Downward R. A. 1982; Molecular homology and incompatibility in the IncFI plasmid group. J Gen Microbiol 128:223–238
     [Google Scholar]
  5. Bergquist P. L., Saadi S., Mass W. K. 1986; Distribution of basic replicon having homology with RepIA, RepIB and RcpIC among IncF group plasmids. Plasmid 15:19–34
     [Google Scholar]
  6. Bharathi A., Polasa H. 1991; Elimination of broad host range plasmid vectors in Escherichia coli by curing agents. FEMS Microbiol Lett 84:37–40
     [Google Scholar]
  7. Birnboim H. C., Doly J. 1979; A rapid alkaline procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523
     [Google Scholar]
  8. Bolivar F., Rodriguez R. L., Greene M. C., Betlach H. L., Boyer H. W., Crosa J. H., Falkow S. 1977; Construction and characterisation of new cloning vehicles. II. A multipurpose cloning system. Gene 2:95–113
     [Google Scholar]
  9. Caro L., Churchward G., Chandler M. 1984; Study of plasmid replication in vivo . Methods Microbiol 17:72–122
     [Google Scholar]
  10. Caughey P. A., de Feyter R., Lane H. E. D. 1986; The miniF plasmid C protein: sequence, purification and DNA binding. Nucleic Acids Res 14:9699–9712
     [Google Scholar]
  11. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12:387–395
     [Google Scholar]
  12. Durga R., Sridhar P., Polasa H. 1990; Effect of plumbagin on antibiotic resistance in bacteria. Indian J Med Res 90:18–20
     [Google Scholar]
  13. Durga R., Sridhar P., Polasa H. 1992; Antimutagenic activity of plumbagin in Salmonella typhimurium test. Indian J Med Res 96:143–145
     [Google Scholar]
  14. Eichenlaub R., Figurski D., Helinski D. R. 1977; Bidirectional replication from a unique origin in miniF plasmid. Proc Natl Acad Sci USA 741138–1141
     [Google Scholar]
  15. Farr S. B., Natvig D. O., Kogma T. 1985; Toxicity and mutagenicity of plumbagin and induction of possible new DNA repair pathway in Escherichia coli . J Bacterial 164:1309–1316
     [Google Scholar]
  16. Figurski D., Kolter R., Meyer R., Kahn M., Eichenlaub R., Helinski D. R. 1978; Replication regions of plasmids ColE1, F, R6K and RK2. In Microbiology 1978 pp. 105–109 Schlessinger D. Edited by Washington, DC: American Society for Microbiology;
     [Google Scholar]
  17. Fujii N., Yamashita Y., Arima Y., Nagashima M., Nakano H. 1992; Induction of topoisomerase II mediated DNA cleavage by the plant naphthoquinones plumbagin and shikonin. Antimicrob Agents Chemother 36:2589–2594
     [Google Scholar]
  18. Gerdes K., Erik J., Larsen L., Molins S. 1985; Stable inheritance of plasmid R1 requires two different loci. J Bacterial 161:292–298
     [Google Scholar]
  19. Gerdes K., Poulsen L. K., Thisted T., Nielsen A. K., Martinussen J., Andreasen P. H. 1990; The hok killer gene family in Gramnegative bacteria. New Biol 2:946–956
     [Google Scholar]
  20. Hohn B., Korn D. 1969; Cosegregation of a sex factor with the Escherichia coli chromosome during curing by acridine orange. J Mol Biol 45:385–395
     [Google Scholar]
  21. Jaffe A., Ogura T., Hiraga S. 1985; Effects of the ccd function of the F plasmid on bacterial growth. J Bacteriol 163:841–849
     [Google Scholar]
  22. Kahn M., Kolter R., Thomas C., Figurski D., Meyer R., Remaut E., Helinski D. R. 1979; Plasmid cloning vehicles derived from ColE1, F, R6K and RK2. Methods Enzymol 68:268–280
     [Google Scholar]
  23. Kline B. C. 1985; A review of mini F plasmid maintenance. Plasmid 14:1–16
     [Google Scholar]
  24. Krishnaswamy M., Purushothaman K. K. 1980; Plumbagin a study of its anticancer, antibacterial and antifungal properties. Indian J Exp Biol 18:876–877
     [Google Scholar]
  25. Lakshmi V. V., Sridhar P., Polasa H. 1986; Elimination of multidrug resistant plasmid in bacteria by plumbagin, a compound derived from a plant. Curr Microbiol 16:159–161
     [Google Scholar]
  26. Lakshmi V. V., Sridhar P., Polasa H. 1989; Loss of plasmid linked antibiotic resistance in Escherichia coli on treatment with phenolic compounds. FEMS Microbiol Lett 57:275–278
     [Google Scholar]
  27. Lane H. E. D. 1981; Replication and incompatibility of F and plasmids in the IncFI group. Plasmid 5:100–126
     [Google Scholar]
  28. Lane D., Gardner R. C. 1979; Second EcoRI fragment of F capable of self-replication. J Bacteriol 139:141–151
     [Google Scholar]
  29. Lane D., Hill D., Caughey P., Gunn P. 1984; The miniF primary origin: sequence analysis and multiple activities. J Mol Biol 180:267–282
     [Google Scholar]
  30. Lane D., de Feyter R., Kennedy M., Phua S.-H., Semon D. 1986; D protein of miniF plasmid acts as a repressor of transcription and as a site-specific resolvase. Nucleic Acids Res 14:9713–9728
     [Google Scholar]
  31. Liu L. F. 1989; DNA ropoisomerase poisons as anti tumor drugs. Annu Res Biochem 58:351–357
     [Google Scholar]
  32. Lovett M. A., Helinski D. R. 1976; Method tor isolation of the replication region of a bacterial replicon: construction of a mini F´km plasmid. J Bacteriol 127:982–989
     [Google Scholar]
  33. Manis J. J., Kline B. C. 1978; F plasmid incompatibility and copy number genes: their map locations and interactions. Plasmid 1:492–507
     [Google Scholar]
  34. Meyer R., Figurski D., Helinski D. R. 1977; Physical and genetic studies with restriction endonucleases on the broad host range plasmid RK2. Mol Gen Genet 152:129–135
     [Google Scholar]
  35. Miki T., Yoshioko K., Horiuchi T. 1984; Control of cell division by sex factor in E.coli. . J Mol Biol 174:605–646
     [Google Scholar]
  36. Miller J. F., Malamy M. H. 1986; Mutational and in vivo methylation analysis of F-factor PifC protein binding to the pifCoperator and the region containing the primary origin of mini-F replication. Proc Natl Acad Sci USA 831433–1437
     [Google Scholar]
  37. Mori H., Kondo A., Ohshima A., Ogura T., Hiraga S. 1986; Structure and function of the F genes essential for partitioning. J Mol Biol 192:1–15
     [Google Scholar]
  38. Murotsu T., Matsubara K., Sugisaki H., Takanami M. 1981; Nine unique repeating sequences in a region essential for replication and incompatibility of the mini-F plasmid. Gene 15:257–271
     [Google Scholar]
  39. Ogura T., Hiraga S. 1983a; Partition mechanism of F plasmid: two plasmid gene-encoded products and a cis-acting site are involved in partition. Cell 32:351–360
     [Google Scholar]
  40. Ogura T., Hiraga S. 1983b; Mini-F plasmid genes that couple host cell division to plasmid proliferation. Proc Natl Acad Sci USA 804784–4788
     [Google Scholar]
  41. Rotman G. S., Cooney R., Malamy M. H. 1983; Cloning of the pif region of the F sex factor and identification of a pif protein product. J Bacteriol 155:254–264
     [Google Scholar]
  42. Stanisich V. A. 1988; Identification and analysis of plasmids on a genetic level. Methods Microbiol 21:11–47
     [Google Scholar]
  43. Tanimoto K., lino T. 1984; An essential gene for replication of the mini-F plasmid from origin I. Mol Gen Genet 196:59–63
     [Google Scholar]
  44. Tolun A., Helinski D. R. 1982; Separation of the minimal replication region of the F plasmid into a replication origin segment and a trans-acting segment. Mol Gen Genet 186:372–377
     [Google Scholar]
  45. Trevors J. T. 1986; Plasmid curing in bacteria. FEMS Microbiol Rev 32:149–157
     [Google Scholar]
  46. Wechsler J., Kline B. C. 1980; Mutation and identification of the F plasmid locus determining resistance to acridine orange curing. Plasmid 4:276–280
     [Google Scholar]
  47. Willshaw G. A., Smith H. R., Anderson E. S. 1978; Molecular studies of F1me resistance plasmids, particularly in epidemic Salmonella typhimurium . Mol Gen Genet 159:111–116
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-142-9-2399
Loading
Curing of F-like plasmid TP181 by plumbagin is due to interference with both replication and maintenance functions
Microbiology 142, 2399 (1996); https://doi.org/10.1099/00221287-142-9-2399
/content/journal/micro/10.1099/00221287-142-9-2399
/content/journal/micro/10.1099/00221287-142-9-2399
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error