A bacteriophage, øBHG1, was isolated from a small eutrophic pond from which its host, Rhodopseudomonas blastica, was originally obtained. It is a lytic bacteriophage specific for R. blastica which also causes non-specific lysis of Rhodobacter sphaeroides 8253. øBHG1 has an icosahedral head of 62 nm diameter and a short 39 nm tail. Caesium chloride density gradient centrifugation of infected cell lysates gave a single bacteriophage band at a density of 1.385 g cm-3, but also occasionally a second band was observed at a lower density. No differences were apparent between bacteriophage taken from either of the two bands. øBHG1 contained double-stranded DNA with a size of 48 kb and a G + C content of 50.6 mol%. The bacteriophage adsorbed to both photosynthetically and chemoheterotrophically grown R. blastica at an identical rate of 1.39 × 10-9 ml−1 min−1. One-step growth curves and kinetic studies of the bacteriophage under these physiological regimes showed no differences in the latent and rise periods and only slight changes in the burst size. Adsorption of this bacteriophage is cell-surface specific and attachment only occurs to the ‘older’ pole of the budding reproductive cell.
AbeliovichA., KaplanS. Bacteriophage of Rhodo-pseudomonas sphaeroides: isolation and characterisation of a Rhodo-pseudomonas sphaeroides bacteriophage. J Virol1974; 13:1392–1399
DuchowG., KohringG.W., GiffhornF. Virulence as a consequence of genome instability of a novel temperate bacteriophage zRsGl of Rhodobacter sphaeroides. Arch Microbiol1985; 142:141–147
DuchrowM., GiffhornF. Physical map of the Rhodobacter sphaeroides bacteriophage zRsGl genome and location of the prophage on the host chromosome. J Bacteriol1987; 169:4410–4414
LoganK.B., ScottG.E., SeeleyN.D., PrimroseS.B. A portable device for the rapid concentration of viruses from large volumes of natural water. J Virol Methods1981; 3:241–249
MandelM., SchildkrautC.L., 81 MarmurJ. Use of caesium chloride density gradient analysis for determining the guanine plus cytosine content of DNA. Methods Enzymol1968; 12B:184–195
SternbergN., TiemeierD., EnquistL. In vitro packaging of a A Dam vector containing EcoRI DNA fragments of Escherichia coli and phage PI. Gene1977; 1:255–280
TuckerW.T., PembertonJ.M. Viral R plasmid Rz6P. Properties of the penicillinase plasmid prophage and supercoiled circular encapsulated genome. J Bacteriol1978; 135:207–214
WhittenburyR., DowC.S. Morphogenesis and differentiation in Rhodomicrobium vannielii and other budding and prosthecate bacteria. Bacteriol Kev1977; 41:754–808