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Volume 142, Issue 4

A 28 kDa major immunogen of shares identity with Mip proteins of spp. and - cloning and characterization of the -like gene Free

Abstract

strain guinea-pig inclusion conjunctivitis (GPIC) produces a self-limiting ocular infection of guinea-pigs, and this condition is a representative animal model of ocular chlamydial disease. Convalescent guinea-pigs, which are resistant to reinfection, produce antibodies to several elementary-body proteins, including an uncharacterized antigen of 28 kDa. Convalescent guinea-pig sera were used to identify, from a lambda expression library, two overlapping GPIC genomic clones that produced the 28 kDa antigenic protein. Nucleotide sequence analysis revealed that the gene coding for the 28 kDa protein was similar to the (macrophage infectivity potentiator) genes from and . The GPIC gene and its product were accordingly designated and Mip, respectively. Analysis of the regions flanking identified three tightly linked open reading frames coding for predicted products with sequence similarity to asparagine tRNA ligase (AspS), rRNA methylase (SpoU), and thioredoxin (TrxA). The arrangement of these genes in GPIC was . Sequence analysis of PCR products produced using genomic DNA from an ovine abortion strain of and from strain LGV-434 demonstrated that the arrangement of and is common among these chlamydiae.

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  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Chlamydia , chlamydial antigen , intracellular pathogen , intracellular survival and Mip-like protein
© Society for General Microbiology 1996
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1996-04-01
2024-04-19
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A 28 kDa major immunogen of Chlamydia psittaci shares identity with Mip proteins of Legionella spp. and Chlamydia trachomatis - cloning and characterization of the C. psittaci mip-like gene
Microbiology 142, 945 (1996); https://doi.org/10.1099/00221287-142-4-945
/content/journal/micro/10.1099/00221287-142-4-945
/content/journal/micro/10.1099/00221287-142-4-945
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