1887

Abstract

A number of strains capable of synthesizing the bacterial exopolysaccharide gellan and related polymers were shown to possess constitutive gellanase activity. In each case, the degradation of deacylated gellan was due to extracellular, eliminase-type enzymes (lyases) which cleave the sequence …ß-D-glucosyl 1,4-ß-D-glucuronosyl… in the tetrasaccharide repeat unit of the substrate polysaccharides. Deacetylated rhamsan was an alternative substrate but there was little or no action against most other polysaccharides with similar structures. Slight differences were found between the specificities of the lyases from different strains. Activities of gellan lyase preparations were generally low. As well as the extracellular ‘gellanase’ activity, all the bacteria possessed varying amounts of ß-D-glucosidase and ß-D-glucuronidase activities apparently located in the periplasm. The products from deacylated gellan and the chemically deacylated form of polysaccharide S194 (rhamsan gum), which is effectively a gentiobiosylated form of gellan, closely resembled those recently obtained by the authors from other, gellandegrading, non-gellan-producing bacteria. The enzymes had negligible activity against the natural, acylated gellan and rhamsan polysaccharides from bacteria now designated as strains of .

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1996-04-01
2024-04-25
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References

  1. Anson A., Fisher P.J., Kennedy A.F.D., Sutherland I.W. A bacterium yielding a polysaccharide with unusual properties. J Appl Bacteriol 1987; 62:147–150
    [Google Scholar]
  2. Baird J.K., Sandford P.A., Cottrel I.W. Industrial applications of some new microbial polysaccharides. BioTechnology 1983; 1:778–783
    [Google Scholar]
  3. Cairns P., Miles M.J., Morris V.J. X -ray fibre diffraction studies of members of the gellan family of polysaccharides. Carbohjdr Polym 1991; 14:367–372
    [Google Scholar]
  4. Crescenzi V., Dentini M., Dea I.C.M. The influence of side-chains on the dilute solution properties of three structurally related, bacterial anionic polysaccharides. Carbohydr Res 1987; 160:283–302
    [Google Scholar]
  5. Davidson I.W., Sutherland I.W., Lawson C.J. Localization of O-acetyl groups of bacterial alginate. J Gen Microbiol 1977; 98:603–606
    [Google Scholar]
  6. Glucksmann M.A., Reuber T.L., Walker G.C. Genes needed for the modification, polymerization, export and processing of succinoglycan by Rhizobium meliloti: a model for succinoglycan biosynthesis. J Bacteriol 1993; 175:7045–7055
    [Google Scholar]
  7. Jansson P.-E., Lindberg B., Sandford P.A. Structural studies of gellan gum, an extracellular polysaccharide elaborated by Pseudomonas elodea. Carbohydr Res 1983; 124:135–139
    [Google Scholar]
  8. Jansson P.-E., Lindberg B., Widmalm G., Sandford P.A. Structural studies of an extracellular polysaccharide (SI30) elaborated by Alcaligenes ATCC 3155. Carbohydr Res 1985; 139:217–223
    [Google Scholar]
  9. Jansson P.-E., Kumar N.S., Lindberg B. Structural studies of a polysaccharide (S-88) elaborated by Pseudomonas ATCC 31554. Carbohydr Res 1986a; 156:165–172
    [Google Scholar]
  10. Jansson P.-E., Lindberg B., Lindberg J., Mackawa E., Sandford P.A. Structural studies of a polysaccharide (SI94) elaborated by Alcaligenes ATCC 31961. Carbohydr Res 1986b; 156:157–163
    [Google Scholar]
  11. Kennedy A.F.D., Sutherland I.W. Analysis of bacterial exopolysaccharides. Biotechnol Appl Biochem 1987; 9:12–19
    [Google Scholar]
  12. Kennedy L., Sutherland I.W. Gellan lyases - novel polysaccharide lyases. Microbiology 1994; 140:3007–3013
    [Google Scholar]
  13. Kennedy L., McDowell K., Sutherland I.W. Alginases from Azotobacter species. J Gen Microbiol 1992; 138:2465–2471
    [Google Scholar]
  14. Kuo M.-S., Mort A.J., Dell A. Identification and location of L-glvcerate, an unusual acvl substituent in gellan gum. Carbohydr Res 1986; 156:173–187
    [Google Scholar]
  15. Lee E.J., Chandrasekaran R. X-ray and computer modelling studies on gellan-related polymers: molecular structures of welan, S-657, and rhamsan. Carbohydr Res 1991; 214:11–24
    [Google Scholar]
  16. Linhardt R.J., Galliher P.M., Cooney C.L. Polysaccharide lyases. Appl Biochem Biotechnol 1986; 12:135–176
    [Google Scholar]
  17. Lobas D., Nimtz M., Wray V., Schumpe A., Proppe C., Deckwer W.-D. Structure and physical properties of the extracellular polysaccharide PS-P4 produced by Sphingomonas paucimobilis P4 (DSM6418). Carbohydr Res 1994; 251:303–318
    [Google Scholar]
  18. Martins L.O., Sz-Correia I. Temperature profiles of gellan gum synthesis and activities of biosynthetic enzymes. Biotechnol Appl Biochem 1994; 20:385–395
    [Google Scholar]
  19. Mattysse A.G., White S., Lightfoot R. Genes required for cellulose synthesis in Agrobacterium tumefaciens. J Bacteriol 1995; 177:1069–1075
    [Google Scholar]
  20. Mikolajczak M.J., Thorne L., Pollock T.J., Armentrout R.W. Sphinganase, a new endoglycanase that cleaves specific members of the gellan family of polysaccharides. Appl Environ Microbiol 1994; 60:402–407
    [Google Scholar]
  21. Pollock T.J. Gellan-related polysaccharides and the genus Sphingomonas. J Gen Microbiol 1993; 139:1939–1945
    [Google Scholar]
  22. Preiss J, Ashwell G. Polygalacturonic acid metabolism in bacteria II Formation and metabolism of 3-deoxy-D-glycero-2, 5-hexodiulosonic acid. J Biol Chem 1963; 238:1577–1583
    [Google Scholar]
  23. Schmedding D.J.M., Van Den Dool R.T.M., Kerkenaar A. Characterisation of a Gelrite depolymerizing enzyme 1987 Abstract B47, Proceedings of Eurocarb IV, Darmstadt
    [Google Scholar]
  24. Standal R., Iverson T.-G., Coucheron D.H., Fjaervik E., Blatny J.M., Valla S. A new gene required for cellulose production and a gene encoding cellulolytic activity in Acetobacter xylinum are colocalized with the bcs operon. J Bacteriol 1994; 176:665–672
    [Google Scholar]
  25. Stankowski J.D., Zeller S.G. Location of a second O-acetyl group in welan by the reductive cleavage method. Carbohydr Res 1992; 224:337–341
    [Google Scholar]
  26. Sutherland I.W. Xanthan lyases - novel enzymes found in various bacterial species. J Gen Microbiol 1987; 133:3129–3134
    [Google Scholar]
  27. Sutherland I.W. Biotechnology of Microbial Exopolysaccharides 1990 Cambridge: Cambridge University Press;
    [Google Scholar]
  28. Sutherland I.W. Structure-function relationships in microbial exopolysaccharides. Biotechnol Adv 1994; 12:393–448
    [Google Scholar]
  29. Sutherland I.W. Polysaccharide lyases. FEMS Microbiol Rev 1995; 12:323–347
    [Google Scholar]
  30. Sutherland I.W., Macdonald R.M. Extracellular enzyme isolation and purification from exopolysaccharide-producing bacteria. J Microbiol Meth 1986; 6:27–31
    [Google Scholar]
  31. Sutherland I.W., Wilkinson J.F. Depolymerases for bacterial exopolysaccharides obtained from phage-infected bacteria. J Gen Microbiol 1965; 39:373–383
    [Google Scholar]
  32. Weissbach A., Hurwitz J. The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli. J Biol Chem 1958; 234:705–709
    [Google Scholar]
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