1887

Abstract

Homochiral--cyclohexa-3,5-diene-1,2-diols are important synthons. We found a way to produce -configured homochiral diols using recombinant 62-1. Transformation of this mutant (Phe Trp Tyr) with plasmids carrying genes involved in chorismic and isochorismic acid metabolism leads to the production of either (+)--(2S,3S)-2,3-dihydroxycyclohexa-4,6-dienecarboxylic acid or (-)--(3R,4R)-3,4-dihydroxycyclohexa-1,5-dienecarboxylic acid, with a yield of 70 or 90 mg (l culture broth), respectively. The metabolic shift from one diene to the other is caused by a change in activity of isochorismate hydroxymutase and/or isochorismatase which in turn results from growth under iron deficiency or overexpression of genes and/or involved in chorismate metabolism.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-142-4-1005
1996-04-01
2019-11-21
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-142-4-1005
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error