1887

Abstract

SUMMARY: The morphology and ultrastructure of two strains of Borrelia anserina were investigated by electron microscopy of negatively stained and ultrathin sectioned cells. One was a cultivable strain originally isolated in the USA and the other was originally isolated in Nigeria and maintained in chickens. The cells were regularly helical, 9-21 μm long and 0·22-0·26 μm wide with a helix wavelength of about 1·7 μm. The cells were surrounded by a surface layer and appeared to divide by binary fission. The structure of the cells from each of the two strains was identical except that those of the USA strain possessed seven flagella inserted at each end and those from the Nigerian strain had eight.

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1995-01-01
2021-07-30
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References

  1. Anderson J. F., Johnson R. C., Magnarelli L. A., Hyde F. W. 1986; Involvement of birds in the epidemiology of the Lyme Disease agent Borrelia burgdorferi . Infect Immun 51:394–396
    [Google Scholar]
  2. Barbour A. G., Hayes S. F., Heiland R. A., Schrumpf M. E., Tessier S. L. 1986; A Borrelia-specific monoclonal antibody binds to a flagellar epitope. Infect Immun 52:549–554
    [Google Scholar]
  3. Burgdorfer W., Barbour A. G., Hayes S. F., Benach J. L., Grunwaldt E., Davis J. P. 1982; Lyme disease, a tick-borne spirochetosis?. Science 6:1317–1319
    [Google Scholar]
  4. DaMassa A. J., Adler H. E. 1979; Avian spirochetosis: natural transmission by Argas (Persicargas) sanchezi (Ixodoidea: Argasidae) and existence of different serologic and immunologic types of Borrelia anserina in the United States. Am J Vet Res 40:154–157
    [Google Scholar]
  5. Felsenfeld O. 1971 In Borrelia. Strains, Vectors, Human and Animal Borreliosis139–142 St Louis, MI, USA: Warren H. Green;
    [Google Scholar]
  6. Fiehn N. E. 1989; Small-sized oral spirochetes and periodontal disease. APMIS, Suppl 7:no. 974–31
    [Google Scholar]
  7. Frasca J. M., Parks V. R. 1965; A routine technique for double-staining ultrathin sections using uranyl and lead salts. J Cell Biol 25:157–161
    [Google Scholar]
  8. Hovind-Hougen K. 1974; Electron microscopy of Borrelia merionesi and Borrelia recurrentis . Acta Pathol Microbiol Scand Sect B Microbiol 82:799–809
    [Google Scholar]
  9. Hovind-Hougen K. 1976; Determinations by means of electron microscopy of morphological criteria of value for classification of some spirochetes, in particular treponemes. Acta Pathol Microbiol Scand Sect B Microbiol Suppl:no. 2551–41
    [Google Scholar]
  10. Hovind-Hougen K. 1984; Ultrastructure of spirochetes isolated from Ixodes ricinus and Ixodes dammini . Yale J Biol Med 57:543–548
    [Google Scholar]
  11. Hovind-Hougen K., Birch-Andersen A. 1971; Electron microscopy of endoflagella and microtubules in Treponema Reiter . Acta Pathol Microbiol Scand Sect B Microbiol 79:37–50
    [Google Scholar]
  12. Hovind-Hougen K., Åsbrink E., Stiernstedt G., Steere A. C., Hovmark A. 1986; Ultrastructural differences among spirochetes isolated from patients with Lyme disease and related disorders, and from Ixodes ricinus . Zentralbl Bakteriol Mikrobiol Hyg Ser A 263:103–111
    [Google Scholar]
  13. Karimi Y., Hovind-Hougen K., Birch-Andersen A., Asmar M. 1979; Borrelia persica and B. baltazardi sp. nov.: Experimental pathogenicity for some animals and comparison of the ultrastructure. Ann Microbiol (Paris) 130B:157–168
    [Google Scholar]
  14. Karlsson M., Hovind-Hougen K., Svenungsson B., Stiernstedt G. 1990; Cultivation and characterization of spirochetes from cerebrospinal fluid of patients with Lyme Borreliosis. J Clin Microbiol 28:473–479
    [Google Scholar]
  15. Kelly R. T. 1984 Borrelia. In Bergey’s Manual of Systematic Bacteriology vol 1 pp 59–62 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  16. Leeflang P., llemobade A. A. 1977; Tick-borne diseases of domestic animals in Northern Nigeria. II. Research summary, 1966 to 1976. Trop Anim Health Prod 9:211–218
    [Google Scholar]
  17. Levine J. F., Dykstra M. J., Nicholson W. L., Walker R. L., Massey G. 1990; Attenuation of Borrelia anserina by serial passage in liquid medium. Res Vet Sci 48:64–69
    [Google Scholar]
  18. Reynolds E. S. 1963; The use-of lead citrate at a high pH as an electron opaque stain in electron microscopy. J Cell Biol 17:208–212
    [Google Scholar]
  19. Soni J. L., Joshi A. G. 1980; A note on strain variation in Akola and Jabalpur strains of Borrelia anserina . Zentralbl Veterinaermed Reihe B 27:70–72
    [Google Scholar]
  20. Walker R. L., Greene R. T., Nicholson W. L., Levine J. F. 1989; Shared flagellar epitopes of Borrelia burgdorferi and Borrelia anserina . Vet Microbiol 19:361–371
    [Google Scholar]
  21. Wouda W., Schillhorn van Veen T. W., Barnes H. J. 1975; Borrelia anserina in chickens previously exposed for Borrelia theileri . Avian Dis 19:209–210
    [Google Scholar]
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