1887

Abstract

SUMMARY:

Synchronous growth studies are often used to assess the presence, timing and duration of periodic phenomena in the bacterial cell cycle. In an effort to evaluate the quality and quantity of information on cycle-specific events that can reasonably be expected from such inquiries, a model was constructed of a synchronous culture of Escherichia coli cells as would be derived from a growing population immobilized on a surface, and applied to the case of one stable and one unstable cellular component. The results indicated that, while the presence of cycle-specific events may be easily detectable, their timing and duration are very difficult to establish in synchronous growth experiments. Furthermore, differences in timing can be misconstrued as differences in duration, and vice versa, when interpretations are based on the qualitative analysis of the data.

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/content/journal/micro/10.1099/00221287-141-1-59
1995-01-01
2021-05-15
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References

  1. Allman R., Schjerven T., Boye E. 1991; Cell cycle parameters of Escherichia coli K-12. J Bacteriol 173:7970–7974
    [Google Scholar]
  2. Campbell J., Kleckner N. 1990; E. coli oriC and the dnaA gene promoter are sequestered from dam methyltransferase following passage of the chromosomal replication fork. Cell 62:967–979
    [Google Scholar]
  3. Cooper S., Helmstetter C. E. 1968; Chromosome replication and the division cycle of Escherichia coli B/r. J Mol Biol 31:519–540
    [Google Scholar]
  4. Edwards C., Porter J., Saunders J. R., Diaper J., Morgan J. A. W., Pickup R. W. 1992; Flow cytometry and microbiology. SGMQ Nov. 1992:105–108
    [Google Scholar]
  5. Figdor C. G., Olijhoek A. J. M., Klencke S., Nanninga N., Bont W. S. 1981; Isolation of small cells from an exponentially growing culture of Escherichia coli by centrifugal elutriation. FEMS Microbiol Lett 10:349–352
    [Google Scholar]
  6. Garrido T., Sanchez M., Palacios P., Aldea M., Vicente M. 1993; Transcription of ftsZ oscillates during the cell cycle of Escherichia coli. EMBO J 12:3957–3965
    [Google Scholar]
  7. Helmstetter C. E. 1967; Rates of DNA synthesis during the division cycle of Escherichia coli B/r. J Mol Biol 24:417–427
    [Google Scholar]
  8. Helmstetter C. E. 1969; Methods for studying the microbial division cycle. Methods Microbiol327–363
    [Google Scholar]
  9. IMSL 1989 Routine UNLSF, IMSL Math/Library, Version 1.1. pp 835–840 Houston; IMSL:
    [Google Scholar]
  10. Leonard A. C., Helmstetter C. E. 1986; Cell cycle-specific replication of Escherichia coli minichromosomes. Proc Natl Acad Sci USA 83:5101–5105
    [Google Scholar]
  11. Sun L., Fuchs J. A. 1992; Escherichia coli ribonucleotide reductase expression is cell cycle regulated. Mol Biol Cell 3:1095–1105
    [Google Scholar]
  12. Theisen P. W., Grimwade J. E., Leonard A. C., Bogan J. A., Helmstetter C. E. 1993; Correlation of gene transcription with the time of initiation of chromosome replication in Escherichia coli . Mol Microbiol 10:575–584
    [Google Scholar]
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