1887

Abstract

SUMMARY:

A gene encoding a chitinase from BJL200 was cloned and expressed in and Nucleotide sequencing revealed an open reading frame encoding a 55·5 kDa protein of 499 amino acids without a typical signal peptide for export. The cellular localization of the chitinase was studied, using two types of cell fractionation methods and immunocytochemical techniques. These analyses showed that the chitinase is located in the cytoplasm in , whereas it is exported to the periplasm in Analysis of chitinase isolated from periplasmic fractions of carrying the cloned gene showed that export of the enzyme is not accompanied by processing at the N-terminus. The chitinase did not show any of the characteristics that have been proposed to direct the export of other non-processed extracellular proteins such as the haemolysin and might therefore be secreted via a hitherto unknown mechanism.

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1995-01-01
2021-05-14
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