1887

Abstract

Growth of in the presence of valine led to a greater production of spiramycin and excretion of short-chain fatty acids compared with growth on ammonium ions as nitrogen source. We determined the activities of enzymes that lead to the formation of malonyl-CoA, a key intermediate in the biosynthesis of spiramycin. In valine-grown cultures, the activities of acetyl-CoA carboxylase and pyruvate dehydrogenase were increased during the antibiotic production phase. During this idiophase, oxaloacetate dehydrogenase activity was much higher in cultures growing on ammonium compared with valine. Two pathways are proposed concerning the mode of malonyl-CoA formation: a route via oxaloacetate dehydrogenase following growth on ammonium, and a route via acetyl-CoA carboxylase after growth on valine.

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1994-06-01
2024-04-20
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References

  1. Behal V., Jechova V., Vanek Z., Hostalek Z. 1977; Alternative pathways of malonyl-CoA formation in Streptomyces aureofaciens. Phytochemistry 16:347–350
    [Google Scholar]
  2. Khaoua S., Lebrihi A., Laakel M., Schneider F., Germain P., Lefebvre G. 1992; Activation of short chain fatty acids and production of spiramycin by Streptomyces ambofaciens. Appl Microbiol Biotechnol 36:763–767
    [Google Scholar]
  3. Lebrihi A., Germain P., Lefebvre G. 1987; Phosphate repression of cephamycin C and clavulanic acid biosynthesis. Appl Microbiol Biotechnol 26:130–135
    [Google Scholar]
  4. Lebrihi A., Lamsaif D., Lefebvre G., Germain P. 1992; Effect of ammonium ions on spiramycin biosynthesis in Streptomyces ambofaciens. Appl Microbiol Biotechnol 37:382–387
    [Google Scholar]
  5. Neusil J., Hostalek Z. 1986; Enzymes of secondary metabolism and the biosynthesis of macrolide antibiotics. Folia Microbiol 31:402–421
    [Google Scholar]
  6. Omura S., Tanaka Y. 1986; Biosynthesis of tylosin and its regulation by ammonium and phosphate. In Regulation of Secondary Metabolite Formation pp. 306–332 Edited by Kleinkauf H., Dohren H. V. , Dormauer H., Nesmann G. . Berlin: Springer;
    [Google Scholar]
  7. Omura S., Takeshima H., Nakagawa A., Miyazawa J., Pirou T., Lukacs G. 1977; Studies on the biosynthesis of 16 membered macrolide antibiotics using carbon-13 magnetic resonance spectroscopy. Biochemistry 16:2860–2866
    [Google Scholar]
  8. Reed L.J., Williams C. R. 1966; Purification and resolution of the pyruvate dehydrogenase complex (Escherichia colt). Methods Enzymol 9:247–265
    [Google Scholar]
  9. Rose I.A. 1955; Acetate kinase of bacteria. Methods Enzymol 1:591–595
    [Google Scholar]
  10. Vancura A., Rezanka T., Marsalek J., Vancurova I., Kristan V., Basarova G. 1987; Effect of ammonium ions on the composition of fatty acids in Streptomyces fradiae, producer of tylosin. FEMS Microbiol Lett 48:357–360
    [Google Scholar]
  11. Vu-Trong K, Gray P. P. 1987; Influence of ammonium on the biosynthesis of the macrolide antibiotic tylosin. Enzyme Microbiol Technol 9:590–593
    [Google Scholar]
  12. Vu-Trong K, Bhuwapathanapun S., Gray P. P. 1980; Metabolic regulation in tylosin-producing Streptomyces fradiae: phosphate control of tylosin biosynthesis. Antimicrob Agents Chemother 17:519–525
    [Google Scholar]
  13. Vu-Trong K, Bhuwapathanapun S., Gray P. P. 1981; Metabolic regulation in tylosin-producing Streptomyces fradiae: regulatory role of adenylate nucleotide pool and enzymes involved in biosynthesis of tylonolide precursors. Antimicrob Agents Chemother 19:209–212
    [Google Scholar]
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