RT Journal Article SR Electronic(1) A1 Makino, Shio A1 Ito, Norihito A1 Inoue, Toshihiko A1 Miyata, Shigeru A1 Moriyama, RyuichiYR 1994 T1 A spore-lytic enzyme released from Bacillus cereus spores during germination JF Microbiology, VO 140 IS 6 SP 1403 OP 1410 DO https://doi.org/10.1099/00221287-140-6-1403 PB Microbiology Society, SN 1465-2080, AB The exudate of fully germinated spores of Bacillus cereus IFO 13597 in 0·25 M sodium phosphate buffer, pH 7·0, was found to contain a spore-lytic enzyme. This enzyme was found to cause loss of absorbance in coat-stripped spore suspensions and phase-darkening of the spores but had minimal activity on isolated peptidoglycan substrates. The enzyme was purified in an active form and identified as a 24 kDa protein which is either an amidase or a peptidase. The amino-terminal 19 residues had the following sequence: FSNQVIQRGASGEKVIELQ. The spore-lytic enzyme retained its activity in a medium of a relatively high ionic strength containing a non-ionic surfactant such as nonaethyleneglycol n-dodecyl ether. This activity was optimum at a salt concentration of about 30 mM in assay buffer at neutral pH. In contrast to the enzyme in a spore-bound form, the enzyme in solution was shown to be heat-sensitive and was readily inactivated by thiol reagents., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-140-6-1403