Characterization of a Streptomyces-lividans-type site-specific DNA modification system in the avermectin-producer Streptomyces avermitilis permits investigation of two novel giant linear plasmids, pSA1 and pSA2
The degradation of Streptomyces avermitilis DNA samples analysed by conventional pulsed-field gel electrophoresis was shown to be due to Trisdependent, double-strand cleavage. Using alternative electrophoretic conditions, separation of intact DNA molecules was achieved, permitting the identification of two novel giant linear plasmids: the 100 kb pSA1 and 250 kb pSA2. Use of pSA2 DNA as a probe showed that pSA1 does not cross-hybridize indicating that the plasmids are not closely related. The site-specificity of the DNA modifications, which render the DNA susceptible to Tris-dependent cleavage, was found to be essentially identical to that of similar modifications found in the DNA of S. lividans.
BancroftI., WolkC. P., OrenE. V.1989; Physical and genetic maps of the genome of the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120. J Bacteriol 171:5940–5948
BurgR. W., MillerB. M., BakerE. E., BirnbaumJ., CurrieS. A., HartmanR., KongY., MonaghanR. L., OlsonG., PutterI., TunacJ. B., WallickH., StapleyE.O., OiwaR., OmuraS.1979; Avermectins, a new family of potent anthelminthic agents: producing organism and fermentation. Antimicrob Agents Chemother 15:361–367
ChenC. W., YuT.-W., LinY.-S., KieserH. M., HopwoodD. A.1993; The conjugative plasmid SLP2 of Streptomjces lividans is a 505 kb linear molecule. Mol Microbiol 7:925–932
DysonP., BetzlerM., KumarT., SchrempfH.1986; Biochemical and genetic analysis of spontaneous genetic instability and DNA amplification in Streptomjces lividans. In Genetics of Industrial Microorganisms. Proceedings of the Fifth International Symposium, Part B pp. 57–65 Edited by AlacevicM., HranueliD., TomanZ. . Zagreb: Pliva;
HopwoodD. A., KieserT., WrightH. M., BibbM. J.1983; Plasmids, recombination, and chromosome mapping in Streptomjces lividans 66. J Gen Microbiol 129:2257–2269
HopwoodD. A., BibbM. J., ChaterK. F., KieserT., BrutonC. J., KieserH. M., LydiateD. J., SmithC. M., WardJ. M., SchrempfH.1985Genetic Manipulation of Streptomjces: a Laboratory Manual Norwich: The John Innes Foundation;
KieserT., HopwoodD. A., WrightH. M., ThompsonC. J.1982; pi J101, a multi-copy broad host-range Streptomjces plasmid: functional analysis and development of DNA cloning vectors. Mol and Gen Genet 185:223–238
KieserH. M., KieserT., HopwoodD. A.1992; A combined genetic and physical map of the Streptomjces coelicolor A 3 (2) chromosome. J Bacteriol 174:5496–5507
KinashiH.1989; Giant linear plasmids in various methylenomycin-producing strains of Streptomjces species. In Genetics and Molecular Biology of Industrial Microorganisms pp. 141–146 Edited by HershbergerC. L., QueenerS. W. , HegemanG. . Washington, DC: American Society for Microbiology;
MatthewM. K., HuiC.-F., SmithC. L., CantorC. R.1988; High resolution separation and accurate size determination in pulsed field gel electrophoresis of DNA. IV. The influence of DNA topology. Biochemistry 27:9222–9226
NasmythK.A.1982; The regulation of yeast mating-type chromatin structure by SIR: an action at a distance affecting both transcription and transposition. Cell 30:567–578
SteinD. S., KendallK. J., CohenS. N.1989; Identification and analysis of transcriptional regulatory signals for the kil and kor loci of Streptomjces plasmid pIJlOl. J Bacteriol 171:5768–5775
TibbettsM. W., HafnerE. W., MorgensteinM. R., SkinnerD. D., DenoyaC. D.1992; Cloning of a DNA fragment involved in pigment production in Streptomjces avermitilis. FEMS Microbiol Lett 91:9–14
VivianA.1971; Genetic control of fertility in Streptomjces coelicolor A 3 (2): plasmid involvement in the interconversion of UF and IF strains. J Gen Microbiol 69:353–364
ZhouX., DengZ., FirminI.L., HopwoodD. A., KieserT.1988; Site-specific degradation of Streptomjces lividans DNA during electrophoresis in buffers contaminated with ferrous iron. Nucleic Acids Res 16:4341–4352
Characterization of a Streptomyces-lividans-type site-specific DNA modification system in the avermectin-producer Streptomyces avermitilis permits investigation of two novel giant linear plasmids, pSA1 and pSA2