@article{mbs:/content/journal/micro/10.1099/00221287-140-6-1367, author = "Evans, Meirwyn and Kaczmarek, Frank S. and Stutzman-Engwall, Kim and Dyson, Paul", title = "Characterization of a Streptomyces-lividans-type site-specific DNA modification system in the avermectin-producer Streptomyces avermitilis permits investigation of two novel giant linear plasmids, pSA1 and pSA2", journal= "Microbiology", year = "1994", volume = "140", number = "6", pages = "1367-1371", doi = "https://doi.org/10.1099/00221287-140-6-1367", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-140-6-1367", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "Linear plasmids", keywords = "Streptomyces avermitilis", keywords = "DNA modification", abstract = "The degradation of Streptomyces avermitilis DNA samples analysed by conventional pulsed-field gel electrophoresis was shown to be due to Trisdependent, double-strand cleavage. Using alternative electrophoretic conditions, separation of intact DNA molecules was achieved, permitting the identification of two novel giant linear plasmids: the 100 kb pSA1 and 250 kb pSA2. Use of pSA2 DNA as a probe showed that pSA1 does not cross-hybridize indicating that the plasmids are not closely related. The site-specificity of the DNA modifications, which render the DNA susceptible to Tris-dependent cleavage, was found to be essentially identical to that of similar modifications found in the DNA of S. lividans.", }